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Detection of Reactive Oxygen and Nitrogen Species (ROS/RNS) During Hypersensitive Cell Death.
Methods Mol Biol. 2018; 1743:97-105.MM

Abstract

Reactive oxygen and nitrogen species (ROS/RNS) are signaling molecules involved in a plethora of physiological processes in plants. Especially, ROS and nitric oxide (NO) are key players that are required for programmed cell death (PCD). The PCD associated with the hypersensitive response (HR) has been well characterized and the role of H2O2 and NO as key signaling molecules inducing HR has been established. Localization of ROS and NO production in plant tissues in response to pathogens can be imaged by confocal laser microscopy by using specific fluorescent probes. Deciphering the time and spatial regulation of ROS and NO is very important to establish the cellular response of plants to adverse conditions. This chapter is mainly focused on the imaging of ROS and RNS accumulation in vivo in plant tissues undergoing PCD.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Terrón-Camero LC
Department of Biochemistry and Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín (CSIC), Granada, Spain.
Molina-Moya E
Department of Biochemistry and Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín (CSIC), Granada, Spain.
Sanz-Fernández M
Department of Biochemistry and Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín (CSIC), Granada, Spain.
Sandalio LM
Department of Biochemistry and Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín (CSIC), Granada, Spain.
Romero-Puertas MC
Department of Biochemistry and Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín (CSIC), Granada, Spain. maria.romero@eez.csic.es.

MeSH

ArabidopsisCell DeathFluorescent Antibody TechniqueNitric OxidePlant Physiological PhenomenaReactive Nitrogen SpeciesReactive Oxygen Species

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29332289

Citation

Terrón-Camero, Laura C., et al. "Detection of Reactive Oxygen and Nitrogen Species (ROS/RNS) During Hypersensitive Cell Death." Methods in Molecular Biology (Clifton, N.J.), vol. 1743, 2018, pp. 97-105.
Terrón-Camero LC, Molina-Moya E, Sanz-Fernández M, et al. Detection of Reactive Oxygen and Nitrogen Species (ROS/RNS) During Hypersensitive Cell Death. Methods Mol Biol. 2018;1743:97-105.
Terrón-Camero, L. C., Molina-Moya, E., Sanz-Fernández, M., Sandalio, L. M., & Romero-Puertas, M. C. (2018). Detection of Reactive Oxygen and Nitrogen Species (ROS/RNS) During Hypersensitive Cell Death. Methods in Molecular Biology (Clifton, N.J.), 1743, 97-105. https://doi.org/10.1007/978-1-4939-7668-3_9
Terrón-Camero LC, et al. Detection of Reactive Oxygen and Nitrogen Species (ROS/RNS) During Hypersensitive Cell Death. Methods Mol Biol. 2018;1743:97-105. PubMed PMID: 29332289.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Reactive Oxygen and Nitrogen Species (ROS/RNS) During Hypersensitive Cell Death. AU - Terrón-Camero,Laura C, AU - Molina-Moya,Eliana, AU - Sanz-Fernández,María, AU - Sandalio,Luisa M, AU - Romero-Puertas,María C, PY - 2018/1/15/entrez PY - 2018/1/15/pubmed PY - 2019/1/11/medline KW - Arabidopsis KW - Hypersensitive response KW - Nitric oxide KW - Peroxynitrite KW - Pseudomonas syringae KW - Reactive oxygen species SP - 97 EP - 105 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 1743 N2 - Reactive oxygen and nitrogen species (ROS/RNS) are signaling molecules involved in a plethora of physiological processes in plants. Especially, ROS and nitric oxide (NO) are key players that are required for programmed cell death (PCD). The PCD associated with the hypersensitive response (HR) has been well characterized and the role of H2O2 and NO as key signaling molecules inducing HR has been established. Localization of ROS and NO production in plant tissues in response to pathogens can be imaged by confocal laser microscopy by using specific fluorescent probes. Deciphering the time and spatial regulation of ROS and NO is very important to establish the cellular response of plants to adverse conditions. This chapter is mainly focused on the imaging of ROS and RNS accumulation in vivo in plant tissues undergoing PCD. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/29332289/Detection_of_Reactive_Oxygen_and_Nitrogen_Species__ROS/RNS__During_Hypersensitive_Cell_Death_ DB - PRIME DP - Unbound Medicine ER -