Tags

Type your tag names separated by a space and hit enter

Role of the hippocampal 5-HT1A receptor-mediated cAMP/PKA signalling pathway in sevoflurane-induced cognitivedysfunction in aged rats.
J Int Med Res. 2018 Mar; 46(3):1073-1085.JI

Abstract

Objective This study aimed to evaluate the role of the hippocampal 5-hydroxytryptamine-1A (5-HT1A)-mediated cyclic adenosine monophosphate/protein kinase A (cAMP/PKA) signalling pathway in sevoflurane-induced cognitive dysfunction in aged rats. Methods Sixty 18-month-old Sprague-Dawley rats were divided into the control (n = 30) and experimental (Sev, n = 30) groups. The experimental group inhaled 50% air/oxygen mixture (2 L/min) and 2% sevoflurane for 4 hours. The control group inhaled 50% air/oxygen mixture (2 L/min) for 4 hours. The Morris water maze test was performed The mRNA expression of 5-HT1A receptor, and cAMP PKA, cAMP response element-binding protein (CREB), and phosphorylated CREB (p-CREB) protein expression were determined. Results The escape latency and swimming distance were greater, and the number of crossings of the platform location and time spent in the platform quadrant were less in the Sev group compared with the control group. cAMP, PKA, CREB, and p-CREB protein expression was downregulated in the Sev group 1 day after anaesthesia compared with the control group. Hippocampal 5-HT1A receptor mRNA expression was higher 7 days after anaesthesia compared with the control group. Conclusion Sevoflurane-induced cognitive dysfunction in aged rats may be related to inhibited expression of the hippocampal 5-HT1A receptor-mediated cAMP/PKA signalling pathway.

Authors+Show Affiliations

1 Department of Anesthesiology, Renmin Hospitai of Wuhan University, Wuhan, Hubei Province, China.2 Department of Anesthesiology, The Second Affiliated Hospital of Inner Mongolia Medical University, Huhhot, Inner Mongolia, China.2 Department of Anesthesiology, The Second Affiliated Hospital of Inner Mongolia Medical University, Huhhot, Inner Mongolia, China.2 Department of Anesthesiology, The Second Affiliated Hospital of Inner Mongolia Medical University, Huhhot, Inner Mongolia, China.1 Department of Anesthesiology, Renmin Hospitai of Wuhan University, Wuhan, Hubei Province, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29332488

Citation

Qiu, Yi, et al. "Role of the Hippocampal 5-HT1A Receptor-mediated cAMP/PKA Signalling Pathway in Sevoflurane-induced Cognitivedysfunction in Aged Rats." The Journal of International Medical Research, vol. 46, no. 3, 2018, pp. 1073-1085.
Qiu Y, Wang Y, Wang X, et al. Role of the hippocampal 5-HT1A receptor-mediated cAMP/PKA signalling pathway in sevoflurane-induced cognitivedysfunction in aged rats. J Int Med Res. 2018;46(3):1073-1085.
Qiu, Y., Wang, Y., Wang, X., Wang, C., & Xia, Z. Y. (2018). Role of the hippocampal 5-HT1A receptor-mediated cAMP/PKA signalling pathway in sevoflurane-induced cognitivedysfunction in aged rats. The Journal of International Medical Research, 46(3), 1073-1085. https://doi.org/10.1177/0300060517744037
Qiu Y, et al. Role of the Hippocampal 5-HT1A Receptor-mediated cAMP/PKA Signalling Pathway in Sevoflurane-induced Cognitivedysfunction in Aged Rats. J Int Med Res. 2018;46(3):1073-1085. PubMed PMID: 29332488.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of the hippocampal 5-HT1A receptor-mediated cAMP/PKA signalling pathway in sevoflurane-induced cognitivedysfunction in aged rats. AU - Qiu,Yi, AU - Wang,Ying, AU - Wang,Xiaodong, AU - Wang,Caixia, AU - Xia,Zhong-Yuan, Y1 - 2018/01/14/ PY - 2018/1/16/pubmed PY - 2018/8/31/medline PY - 2018/1/16/entrez KW - 5-hydroxytryptamine-1A receptor KW - Cyclic adenosine monophosphate KW - cerebral hippocampus KW - cognitive dysfunction KW - protein kinase A KW - sevoflurane SP - 1073 EP - 1085 JF - The Journal of international medical research JO - J Int Med Res VL - 46 IS - 3 N2 - Objective This study aimed to evaluate the role of the hippocampal 5-hydroxytryptamine-1A (5-HT1A)-mediated cyclic adenosine monophosphate/protein kinase A (cAMP/PKA) signalling pathway in sevoflurane-induced cognitive dysfunction in aged rats. Methods Sixty 18-month-old Sprague-Dawley rats were divided into the control (n = 30) and experimental (Sev, n = 30) groups. The experimental group inhaled 50% air/oxygen mixture (2 L/min) and 2% sevoflurane for 4 hours. The control group inhaled 50% air/oxygen mixture (2 L/min) for 4 hours. The Morris water maze test was performed The mRNA expression of 5-HT1A receptor, and cAMP PKA, cAMP response element-binding protein (CREB), and phosphorylated CREB (p-CREB) protein expression were determined. Results The escape latency and swimming distance were greater, and the number of crossings of the platform location and time spent in the platform quadrant were less in the Sev group compared with the control group. cAMP, PKA, CREB, and p-CREB protein expression was downregulated in the Sev group 1 day after anaesthesia compared with the control group. Hippocampal 5-HT1A receptor mRNA expression was higher 7 days after anaesthesia compared with the control group. Conclusion Sevoflurane-induced cognitive dysfunction in aged rats may be related to inhibited expression of the hippocampal 5-HT1A receptor-mediated cAMP/PKA signalling pathway. SN - 1473-2300 UR - https://www.unboundmedicine.com/medline/citation/29332488/Role_of_the_hippocampal_5_HT1A_receptor_mediated_cAMP/PKA_signalling_pathway_in_sevoflurane_induced_cognitivedysfunction_in_aged_rats_ L2 - https://journals.sagepub.com/doi/10.1177/0300060517744037?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -