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Expression of sperm PLCζ and clinical outcomes of ICSI-AOA in men affected by globozoospermia due to DPY19L2 deletion.
Reprod Biomed Online. 2018 Mar; 36(3):348-355.RB

Abstract

Globozoospermia is characterized by the presence of 100% acrosomeless round-headed spermatozoa in an ejaculate. Failed fertilization after intracytoplasmic sperm injection (ICSI) is commonly reported for globozoospermic couples and can be overcome by artificial oocyte activation (AOA). Phospholipase C zeta (PLCζ) is one of the main sperm factors involved in oocyte activation and its low expression levels mainly account for fertilization failure. Deletion of the DPY19L2 gene is reported as a main genetic cause in over 70% of infertile men with globozoospermia. The current study assesses the expression profile of sperm PLCζ at RNA and protein levels in 32 DPY19L2 deletion-mediated globozoospermic men and reports corresponding clinical outcomes following ICSI with AOA. The expression of PLCζ relative to GAPDH at RNA (0.78 ± 0.16 versus 1.65 ± 0.24; P = 0.02) and protein (0.39 ± 0.12 versus 0.83 ± 0.13; P = 0.01) levels in globozoospermic men with DPY19L2 deletion was significantly lower compared with fertile men (n = 32). Fertilization rate in globozoospermic couples following ICSI-AOA was significantly lower compared with fertile men (53.14 ± 5.13% versus 87.64 ± 2.38%, P < 0.001). However, implantation (26.2%) and pregnancy (53.8%) rates were not jeopardized by DPY19L2 deletion in these couples.

Authors+Show Affiliations

Department of Reproductive Biotechnology, Reproductive Biomedicine Research Centre, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.College of Medicine, Member of QU Health, Qatar University, PO Box 2713, Doha, Qatar.College of Medicine, Member of QU Health, Qatar University, PO Box 2713, Doha, Qatar; College of Biomedical and Life Sciences, School of Biosciences, Cardiff University, Cardiff, UK.Department of Reproductive Biotechnology, Reproductive Biomedicine Research Centre, Royan Institute for Biotechnology, ACECR, Isfahan, Iran; Isfahan Fertility and Infertility Centre, Isfahan, Iran. Electronic address: mh.nasr-esfahani@royaninstitute.org.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29339016

Citation

Tavalaee, Marziyeh, et al. "Expression of Sperm PLCζ and Clinical Outcomes of ICSI-AOA in Men Affected By Globozoospermia Due to DPY19L2 Deletion." Reproductive Biomedicine Online, vol. 36, no. 3, 2018, pp. 348-355.
Tavalaee M, Nomikos M, Lai FA, et al. Expression of sperm PLCζ and clinical outcomes of ICSI-AOA in men affected by globozoospermia due to DPY19L2 deletion. Reprod Biomed Online. 2018;36(3):348-355.
Tavalaee, M., Nomikos, M., Lai, F. A., & Nasr-Esfahani, M. H. (2018). Expression of sperm PLCζ and clinical outcomes of ICSI-AOA in men affected by globozoospermia due to DPY19L2 deletion. Reproductive Biomedicine Online, 36(3), 348-355. https://doi.org/10.1016/j.rbmo.2017.12.013
Tavalaee M, et al. Expression of Sperm PLCζ and Clinical Outcomes of ICSI-AOA in Men Affected By Globozoospermia Due to DPY19L2 Deletion. Reprod Biomed Online. 2018;36(3):348-355. PubMed PMID: 29339016.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of sperm PLCζ and clinical outcomes of ICSI-AOA in men affected by globozoospermia due to DPY19L2 deletion. AU - Tavalaee,Marziyeh, AU - Nomikos,Michail, AU - Lai,F Anthony, AU - Nasr-Esfahani,Mohammad Hossein, Y1 - 2017/12/29/ PY - 2017/06/24/received PY - 2017/11/28/revised PY - 2017/12/07/accepted PY - 2018/1/18/pubmed PY - 2018/11/6/medline PY - 2018/1/18/entrez KW - DPY19L2 deletion KW - Fertilization KW - Globozoospermia KW - ICSI-AOA KW - PLCζ KW - Pregnancy SP - 348 EP - 355 JF - Reproductive biomedicine online JO - Reprod. Biomed. Online VL - 36 IS - 3 N2 - Globozoospermia is characterized by the presence of 100% acrosomeless round-headed spermatozoa in an ejaculate. Failed fertilization after intracytoplasmic sperm injection (ICSI) is commonly reported for globozoospermic couples and can be overcome by artificial oocyte activation (AOA). Phospholipase C zeta (PLCζ) is one of the main sperm factors involved in oocyte activation and its low expression levels mainly account for fertilization failure. Deletion of the DPY19L2 gene is reported as a main genetic cause in over 70% of infertile men with globozoospermia. The current study assesses the expression profile of sperm PLCζ at RNA and protein levels in 32 DPY19L2 deletion-mediated globozoospermic men and reports corresponding clinical outcomes following ICSI with AOA. The expression of PLCζ relative to GAPDH at RNA (0.78 ± 0.16 versus 1.65 ± 0.24; P = 0.02) and protein (0.39 ± 0.12 versus 0.83 ± 0.13; P = 0.01) levels in globozoospermic men with DPY19L2 deletion was significantly lower compared with fertile men (n = 32). Fertilization rate in globozoospermic couples following ICSI-AOA was significantly lower compared with fertile men (53.14 ± 5.13% versus 87.64 ± 2.38%, P < 0.001). However, implantation (26.2%) and pregnancy (53.8%) rates were not jeopardized by DPY19L2 deletion in these couples. SN - 1472-6491 UR - https://www.unboundmedicine.com/medline/citation/29339016/Expression_of_sperm_PLCζ_and_clinical_outcomes_of_ICSI_AOA_in_men_affected_by_globozoospermia_due_to_DPY19L2_deletion_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1472-6483(17)30708-3 DB - PRIME DP - Unbound Medicine ER -