Tags

Type your tag names separated by a space and hit enter

Regulation of the Response of Caenorhabditis elegans to Simulated Microgravity by p38 Mitogen-Activated Protein Kinase Signaling.
Sci Rep. 2018 01 16; 8(1):857.SR

Abstract

The in vivo function of p38 mitogen-activated protein kinase (MAPK) signaling in regulating the response to simulated microgravity is still largely unclear. Using Caenorhabditis elegans as an assay system, we investigated the in vivo function of p38 MAPK signaling in regulating the response of animals to simulated microgravity and the underlying molecular mechanism. Simulated microgravity treatment significantly increased the transcriptional expressions of genes (pmk-1, sek-1, and nsy-1) encoding core p38 MAPK signaling pathway and the expression of phosphorylated PMK-1/p38 MAPK. The pmk-1, sek-1, or nsy-1 mutant was susceptible to adverse effects of simulated microgravity. The intestine-specific activity of PMK-1 was required for its function in regulating the response to simulated microgravity, and the entire p38 MAPK signaling pathway could act in the intestine to regulate the response to simulated microgravity. In the intestine, SKN-1 and ATF-7, two transcriptional factors, were identified as downstream targets for PMK-1 in regulating the response to simulated microgravity. Therefore, the activation of p38 MAPK signaling may mediate a protection mechanism for nematodes against the adverse effects of simulated microgravity. Additionally, our results highlight the potential crucial role of intestinal cells in response to simulated microgravity in nematodes.

Links

PMC Free PDF

Authors+Show Affiliations

Li W
Medical School, Southeast University, Nanjing, 210009, China.
Wang D
Medical School, Southeast University, Nanjing, 210009, China.
Wang D
Medical School, Southeast University, Nanjing, 210009, China. dayongw@seu.edu.cn.

MeSH

Activating Transcription FactorsAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsDNA-Binding ProteinsIntestinal MucosaMAP Kinase Kinase 4MAP Kinase Signaling SystemMitogen-Activated Protein KinasesProtein Serine-Threonine KinasesRNA InterferenceRNA, Small InterferingReactive Oxygen SpeciesSurvival AnalysisTranscription FactorsWeightlessness Simulation

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29339777

Citation

Li, Wenjie, et al. "Regulation of the Response of Caenorhabditis Elegans to Simulated Microgravity By P38 Mitogen-Activated Protein Kinase Signaling." Scientific Reports, vol. 8, no. 1, 2018, p. 857.
Li W, Wang D, Wang D. Regulation of the Response of Caenorhabditis elegans to Simulated Microgravity by p38 Mitogen-Activated Protein Kinase Signaling. Sci Rep. 2018;8(1):857.
Li, W., Wang, D., & Wang, D. (2018). Regulation of the Response of Caenorhabditis elegans to Simulated Microgravity by p38 Mitogen-Activated Protein Kinase Signaling. Scientific Reports, 8(1), 857. https://doi.org/10.1038/s41598-018-19377-z
Li W, Wang D, Wang D. Regulation of the Response of Caenorhabditis Elegans to Simulated Microgravity By P38 Mitogen-Activated Protein Kinase Signaling. Sci Rep. 2018 01 16;8(1):857. PubMed PMID: 29339777.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of the Response of Caenorhabditis elegans to Simulated Microgravity by p38 Mitogen-Activated Protein Kinase Signaling. AU - Li,Wenjie, AU - Wang,Daoyong, AU - Wang,Dayong, Y1 - 2018/01/16/ PY - 2017/10/11/received PY - 2017/12/29/accepted PY - 2018/1/18/entrez PY - 2018/1/18/pubmed PY - 2018/11/20/medline SP - 857 EP - 857 JF - Scientific reports JO - Sci Rep VL - 8 IS - 1 N2 - The in vivo function of p38 mitogen-activated protein kinase (MAPK) signaling in regulating the response to simulated microgravity is still largely unclear. Using Caenorhabditis elegans as an assay system, we investigated the in vivo function of p38 MAPK signaling in regulating the response of animals to simulated microgravity and the underlying molecular mechanism. Simulated microgravity treatment significantly increased the transcriptional expressions of genes (pmk-1, sek-1, and nsy-1) encoding core p38 MAPK signaling pathway and the expression of phosphorylated PMK-1/p38 MAPK. The pmk-1, sek-1, or nsy-1 mutant was susceptible to adverse effects of simulated microgravity. The intestine-specific activity of PMK-1 was required for its function in regulating the response to simulated microgravity, and the entire p38 MAPK signaling pathway could act in the intestine to regulate the response to simulated microgravity. In the intestine, SKN-1 and ATF-7, two transcriptional factors, were identified as downstream targets for PMK-1 in regulating the response to simulated microgravity. Therefore, the activation of p38 MAPK signaling may mediate a protection mechanism for nematodes against the adverse effects of simulated microgravity. Additionally, our results highlight the potential crucial role of intestinal cells in response to simulated microgravity in nematodes. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/29339777/Regulation_of_the_Response_of_Caenorhabditis_elegans_to_Simulated_Microgravity_by_p38_Mitogen_Activated_Protein_Kinase_Signaling_ DB - PRIME DP - Unbound Medicine ER -