Tags

Type your tag names separated by a space and hit enter

Simultaneous detection of chicken cytokines in plasma samples using the Bio-Plex assay.
Poult Sci. 2018 Apr 01; 97(4):1127-1133.PS

Abstract

A chicken multiplex cytokine assay (Bio-Plex) to detect four different cytokines (IL-2, IL-12, IL-10, and interferon gamma) simultaneously in plasma samples was designed. Most standard curves range between 1 to 5 pg/mL and 5,000 pg/mL, except for IFNγ with the range of 50 to 25,000 pg/mL. Such a chicken multiplex assay proved to be fast and reliable, and comparable in sensitivity, accuracy, and reproducibility to conventional enzyme-linked immunosorbent assays. Comparison of the multiplex assay with the ELISA technique using the same clones of detection and capture antibodies resulted in correlation coefficients for all cytokines ranging from 0.95 to 0.99. Lower limit of detection and limit of quantification values were obtained for all tested cytokines by the Bio-Plex assay compared with ELISA. To reduce the risk of cross-reaction with other proteins, the Bio-Plex system was used, combining the principle of sandwich immunoassay with the Luminex bead-based technology. The cytokine standard recoveries for each cytokine varied between 86 and 118% in dynamic concentration ranges. A chicken multiplex cytokine assay (Bio-Plex) provided a more complete picture of differences between the Th1/Th2 cytokine profiles of the immunized via a new system of antigen delivery into chicken antigen-presenting cells and control groups. This multiplexed fluorescent-bead-based detection assay can be used as a quantitative or comparative tool for the study of the chicken ex vivo cellular immune response.

Authors+Show Affiliations

BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, 25401 Jílové u Prahy, Czech Republic.BIOCEV, Czech Centre for Phenogenomics, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic, Prumyslová 595, 25242 Vestec, Czech Republic.Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Vídenská 1083, 14220 Prague, Czech Republic.BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, 25401 Jílové u Prahy, Czech Republic.BIOCEV, Czech Centre for Phenogenomics, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic, Prumyslová 595, 25242 Vestec, Czech Republic.Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídenská 1083, 14220 Prague, Czech Republic.BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, 25401 Jílové u Prahy, Czech Republic.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

29340698

Citation

Mucksová, Jitka, et al. "Simultaneous Detection of Chicken Cytokines in Plasma Samples Using the Bio-Plex Assay." Poultry Science, vol. 97, no. 4, 2018, pp. 1127-1133.
Mucksová J, Chalupský K, Plachý J, et al. Simultaneous detection of chicken cytokines in plasma samples using the Bio-Plex assay. Poult Sci. 2018;97(4):1127-1133.
Mucksová, J., Chalupský, K., Plachý, J., Kalina, J., Rachacová, P., Stanek, O., & Trefil, P. (2018). Simultaneous detection of chicken cytokines in plasma samples using the Bio-Plex assay. Poultry Science, 97(4), 1127-1133. https://doi.org/10.3382/ps/pex411
Mucksová J, et al. Simultaneous Detection of Chicken Cytokines in Plasma Samples Using the Bio-Plex Assay. Poult Sci. 2018 Apr 1;97(4):1127-1133. PubMed PMID: 29340698.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous detection of chicken cytokines in plasma samples using the Bio-Plex assay. AU - Mucksová,Jitka, AU - Chalupský,Karel, AU - Plachý,Jirí, AU - Kalina,Jirí, AU - Rachacová,Pavla, AU - Stanek,Ondrej, AU - Trefil,Pavel, PY - 2017/05/17/received PY - 2017/12/06/accepted PY - 2018/1/18/pubmed PY - 2018/8/21/medline PY - 2018/1/18/entrez SP - 1127 EP - 1133 JF - Poultry science JO - Poult. Sci. VL - 97 IS - 4 N2 - A chicken multiplex cytokine assay (Bio-Plex) to detect four different cytokines (IL-2, IL-12, IL-10, and interferon gamma) simultaneously in plasma samples was designed. Most standard curves range between 1 to 5 pg/mL and 5,000 pg/mL, except for IFNγ with the range of 50 to 25,000 pg/mL. Such a chicken multiplex assay proved to be fast and reliable, and comparable in sensitivity, accuracy, and reproducibility to conventional enzyme-linked immunosorbent assays. Comparison of the multiplex assay with the ELISA technique using the same clones of detection and capture antibodies resulted in correlation coefficients for all cytokines ranging from 0.95 to 0.99. Lower limit of detection and limit of quantification values were obtained for all tested cytokines by the Bio-Plex assay compared with ELISA. To reduce the risk of cross-reaction with other proteins, the Bio-Plex system was used, combining the principle of sandwich immunoassay with the Luminex bead-based technology. The cytokine standard recoveries for each cytokine varied between 86 and 118% in dynamic concentration ranges. A chicken multiplex cytokine assay (Bio-Plex) provided a more complete picture of differences between the Th1/Th2 cytokine profiles of the immunized via a new system of antigen delivery into chicken antigen-presenting cells and control groups. This multiplexed fluorescent-bead-based detection assay can be used as a quantitative or comparative tool for the study of the chicken ex vivo cellular immune response. SN - 1525-3171 UR - https://www.unboundmedicine.com/medline/citation/29340698/Simultaneous_detection_of_chicken_cytokines_in_plasma_samples_using_the_Bio_Plex_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/4801132 DB - PRIME DP - Unbound Medicine ER -