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Melatonin attenuates lung ischaemia-reperfusion injury via inhibition of oxidative stress and inflammation.
Interact Cardiovasc Thorac Surg. 2018 05 01; 26(5):761-767.IC

Abstract

OBJECTIVES

Lung ischaemia-reperfusion injury is a complex pathophysiological process due to the production of reactive oxygen species and the generation of inflammatory reaction. We investigated the protective effects and the corresponding mechanism of melatonin (MT), a potent free-radical scavenger, on lung injury induced by ischaemia-reperfusion in a mouse model.

METHODS

Adult male C57BL/6J mice (n = 30) were randomly and equally allocated into 5 groups: sham controls, IR, IR + 10 mg/kg MT, IR + 20 mg/kg MT and IR + 30 mg/kg MT. Lung ischaemia-reperfusion injury was induced by thoracotomy followed by clamping of the left hilum for 1 h and subsequent reperfusion for 2 h.

RESULTS

Histological scoring analysis showed that lung parenchymal damage was ameliorated in the melatonin pretreatment groups when compared with the IR group, with the IR + 20 mg/kg MT group showing the strongest effect among the melatonin pretreatment groups. Wet-to-dry weight ratio, detection of malondialdehyde, protein expressions of inflammatory factors (tumour necrosis factor-α, interleukin-1β, NF-κB and IKK-γ) and apoptotic factors (cleaved caspase-3 and Bax/Bcl-2), as well as TUNEL assay showed changes similar to those of the lung injury scores in all groups. In contrast, the examination of superoxide dismutase showed a pattern contrary to that of the lung injury score in all groups. In addition, immunohistochemistry staining showed that the expressions of the antioxidants glutathione peroxidase and glutathione reductase were increased in the melatonin pretreatment groups.

CONCLUSIONS

This study demonstrated that melatonin pretreatment attenuated lung ischaemia-reperfusion injury via inhibition of oxidative stress, inflammation and apoptosis.

Authors+Show Affiliations

Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29346581

Citation

Wang, Ming-Liang, et al. "Melatonin Attenuates Lung Ischaemia-reperfusion Injury Via Inhibition of Oxidative Stress and Inflammation." Interactive Cardiovascular and Thoracic Surgery, vol. 26, no. 5, 2018, pp. 761-767.
Wang ML, Wei CH, Wang WD, et al. Melatonin attenuates lung ischaemia-reperfusion injury via inhibition of oxidative stress and inflammation. Interact Cardiovasc Thorac Surg. 2018;26(5):761-767.
Wang, M. L., Wei, C. H., Wang, W. D., Wang, J. S., Zhang, J., & Wang, J. J. (2018). Melatonin attenuates lung ischaemia-reperfusion injury via inhibition of oxidative stress and inflammation. Interactive Cardiovascular and Thoracic Surgery, 26(5), 761-767. https://doi.org/10.1093/icvts/ivx440
Wang ML, et al. Melatonin Attenuates Lung Ischaemia-reperfusion Injury Via Inhibition of Oxidative Stress and Inflammation. Interact Cardiovasc Thorac Surg. 2018 05 1;26(5):761-767. PubMed PMID: 29346581.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Melatonin attenuates lung ischaemia-reperfusion injury via inhibition of oxidative stress and inflammation. AU - Wang,Ming-Liang, AU - Wei,Chun-Hua, AU - Wang,Wen-Dong, AU - Wang,Jia-Shun, AU - Zhang,Jun, AU - Wang,Jian-Jun, PY - 2017/09/06/received PY - 2017/12/22/accepted PY - 2018/1/19/pubmed PY - 2019/4/10/medline PY - 2018/1/19/entrez SP - 761 EP - 767 JF - Interactive cardiovascular and thoracic surgery JO - Interact Cardiovasc Thorac Surg VL - 26 IS - 5 N2 - OBJECTIVES: Lung ischaemia-reperfusion injury is a complex pathophysiological process due to the production of reactive oxygen species and the generation of inflammatory reaction. We investigated the protective effects and the corresponding mechanism of melatonin (MT), a potent free-radical scavenger, on lung injury induced by ischaemia-reperfusion in a mouse model. METHODS: Adult male C57BL/6J mice (n = 30) were randomly and equally allocated into 5 groups: sham controls, IR, IR + 10 mg/kg MT, IR + 20 mg/kg MT and IR + 30 mg/kg MT. Lung ischaemia-reperfusion injury was induced by thoracotomy followed by clamping of the left hilum for 1 h and subsequent reperfusion for 2 h. RESULTS: Histological scoring analysis showed that lung parenchymal damage was ameliorated in the melatonin pretreatment groups when compared with the IR group, with the IR + 20 mg/kg MT group showing the strongest effect among the melatonin pretreatment groups. Wet-to-dry weight ratio, detection of malondialdehyde, protein expressions of inflammatory factors (tumour necrosis factor-α, interleukin-1β, NF-κB and IKK-γ) and apoptotic factors (cleaved caspase-3 and Bax/Bcl-2), as well as TUNEL assay showed changes similar to those of the lung injury scores in all groups. In contrast, the examination of superoxide dismutase showed a pattern contrary to that of the lung injury score in all groups. In addition, immunohistochemistry staining showed that the expressions of the antioxidants glutathione peroxidase and glutathione reductase were increased in the melatonin pretreatment groups. CONCLUSIONS: This study demonstrated that melatonin pretreatment attenuated lung ischaemia-reperfusion injury via inhibition of oxidative stress, inflammation and apoptosis. SN - 1569-9285 UR - https://www.unboundmedicine.com/medline/citation/29346581/Melatonin_attenuates_lung_ischaemia_reperfusion_injury_via_inhibition_of_oxidative_stress_and_inflammation_ L2 - https://academic.oup.com/icvts/article-lookup/doi/10.1093/icvts/ivx440 DB - PRIME DP - Unbound Medicine ER -