Tags

Type your tag names separated by a space and hit enter

Detection of bovine carriers of Leptospira by serological, bacteriological, and molecular tools.
Trop Anim Health Prod 2018; 50(4):883-888TA

Abstract

Bovine leptospirosis is an important infectious disease that causes reproductive problems and economic risks, particularly in the tropics. The present study aimed to determine the extent of Leptospira infection among bovines on a slaughterhouse from Rio de Janeiro, Brazil via serological, bacteriological, and molecular tests. Two hundred eight bovines were examined in total, and we obtained 208 blood samples for serology, 198 urine samples collected via direct bladder puncture for polymerase chain reaction (PCR) and culture, 208 kidney samples (one from each animal) for PCR and culture, and 92 vaginal fluid samples from sterile swabs for PCR and culture. Serology demonstrated that 77/208 (37%) of the animals presented anti-Leptospira antibodies. Serogroup Sejroe was by far the most common. One hundrd thirty-three animals (63.9%) were PCR positive in at least one of the tested samples and were considered as Leptospira carriers. Furthermore, ten isolates were obtained by pure culture, all of them from urine samples. Bovine leptospirosis is widely prevalent, and the occurrence of renal carriers was unexpectedly much higher than generally reported.

Authors+Show Affiliations

Universidade Federal da Bahia, Av. Adhemar de Barros, 500, Ondina, Salvador, Bahia, CEP: 40170-110, Brazil. melissahp@ufba.br.Laboratório de Bacteriologia Veterinária, Universidade Federal Fluminense, Rua Hernani Melo 101, São Domingos, Niterói, Rio de Janeiro, 24210-130, Brazil.Laboratório de Bacteriologia Veterinária, Universidade Federal Fluminense, Rua Hernani Melo 101, São Domingos, Niterói, Rio de Janeiro, 24210-130, Brazil.Laboratório de Bacteriologia Veterinária, Universidade Federal Fluminense, Rua Hernani Melo 101, São Domingos, Niterói, Rio de Janeiro, 24210-130, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29349716

Citation

Pinna, Melissa H., et al. "Detection of Bovine Carriers of Leptospira By Serological, Bacteriological, and Molecular Tools." Tropical Animal Health and Production, vol. 50, no. 4, 2018, pp. 883-888.
Pinna MH, Martins G, Loureiro AP, et al. Detection of bovine carriers of Leptospira by serological, bacteriological, and molecular tools. Trop Anim Health Prod. 2018;50(4):883-888.
Pinna, M. H., Martins, G., Loureiro, A. P., & Lilenbaum, W. (2018). Detection of bovine carriers of Leptospira by serological, bacteriological, and molecular tools. Tropical Animal Health and Production, 50(4), pp. 883-888. doi:10.1007/s11250-018-1512-z.
Pinna MH, et al. Detection of Bovine Carriers of Leptospira By Serological, Bacteriological, and Molecular Tools. Trop Anim Health Prod. 2018;50(4):883-888. PubMed PMID: 29349716.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of bovine carriers of Leptospira by serological, bacteriological, and molecular tools. AU - Pinna,Melissa H, AU - Martins,Gabriel, AU - Loureiro,Ana Paula, AU - Lilenbaum,Walter, Y1 - 2018/01/18/ PY - 2017/05/04/received PY - 2018/01/05/accepted PY - 2018/1/20/pubmed PY - 2018/8/8/medline PY - 2018/1/20/entrez KW - Diagnostic KW - Isolation KW - Leptospirosis KW - PCR KW - Slaughtered SP - 883 EP - 888 JF - Tropical animal health and production JO - Trop Anim Health Prod VL - 50 IS - 4 N2 - Bovine leptospirosis is an important infectious disease that causes reproductive problems and economic risks, particularly in the tropics. The present study aimed to determine the extent of Leptospira infection among bovines on a slaughterhouse from Rio de Janeiro, Brazil via serological, bacteriological, and molecular tests. Two hundred eight bovines were examined in total, and we obtained 208 blood samples for serology, 198 urine samples collected via direct bladder puncture for polymerase chain reaction (PCR) and culture, 208 kidney samples (one from each animal) for PCR and culture, and 92 vaginal fluid samples from sterile swabs for PCR and culture. Serology demonstrated that 77/208 (37%) of the animals presented anti-Leptospira antibodies. Serogroup Sejroe was by far the most common. One hundrd thirty-three animals (63.9%) were PCR positive in at least one of the tested samples and were considered as Leptospira carriers. Furthermore, ten isolates were obtained by pure culture, all of them from urine samples. Bovine leptospirosis is widely prevalent, and the occurrence of renal carriers was unexpectedly much higher than generally reported. SN - 1573-7438 UR - https://www.unboundmedicine.com/medline/citation/29349716/Detection_of_bovine_carriers_of_Leptospira_by_serological_bacteriological_and_molecular_tools_ L2 - https://dx.doi.org/10.1007/s11250-018-1512-z DB - PRIME DP - Unbound Medicine ER -