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Analysis of phenolic compounds in different parts of pomegranate (Punica granatum) fruit by HPLC-PDA-ESI/MS and evaluation of their antioxidant activity: application to different Italian varieties.
Anal Bioanal Chem. 2018 Jun; 410(15):3507-3520.AB

Abstract

The analysis of pomegranate phenolic compounds belonging to different classes in different fruit parts was performed by high-performance liquid chromatography coupled with photodiode array and mass spectrometry detection. Two different separation methods were optimized for the analysis of anthocyanins and hydrolyzable tannins along with phenolic acids and flavonoids. Two C18 columns, core-shell and fully porous particle stationary phases, were used. The parameters for separation of phenolic compounds were optimized considering chromatographic resolution and analysis time. Thirty-five phenolic compounds were found, and 28 of them were tentatively identified as belonging to four different phenolic compound classes; namely, anthocyanins, phenolic acids, hydrolyzable tannins, and flavonoids. Quantitative analysis was performed with a mixture of nine phenolic compounds belonging to phenolic compound classes representative of pomegranate. The method was then fully validated in terms of retention time precision, expressed as the relative standard deviation, limit of detection, limit of quantification, and linearity range. Phenolic compounds were analyzed directly in pomegranate juice, and after solvent extraction with a mixture of water and methanol with a small percentage of acid in peel and pulp samples. The accuracy of the extraction method was also assessed, and satisfactory values were obtained. Finally, the method was used to study identified analytes in pomegranate juice, peel, and pulp of six different Italian varieties and one international variety. Differences in phenolic compound profiles among the different pomegranate parts were observed. Pomegranate peel samples showed a high concentration of phenolic compounds, ellagitannins being the most abundant ones, with respect to pulp and juice samples for each variety. With the same samples, total phenols and antioxidant activity were evaluated through colorimetric assays, and the results were correlated among them.

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Authors+Show Affiliations

Russo M
Department of Medicine, University Campus Bio-Medico of Rome, Via Álvaro del Portillo 21, 00128, Rome, Italy.
Fanali C
Department of Medicine, University Campus Bio-Medico of Rome, Via Álvaro del Portillo 21, 00128, Rome, Italy. c.fanali@unicampus.it.
Tripodo G
Department of Medicine, University Campus Bio-Medico of Rome, Via Álvaro del Portillo 21, 00128, Rome, Italy.
Dugo P
Department of Medicine, University Campus Bio-Medico of Rome, Via Álvaro del Portillo 21, 00128, Rome, Italy. Chemistry, Biological, Pharmaceutical and Environmental Sciences Department, University of Messina, Polo Annunziata, Viale Annunziata, 98168, Messina, Italy. Chromaleont s.r.l., Viale Boccetta 70, 98122, Messina, Italy.
Muleo R
Department of Agricultural and Forestry Sciences, University of Tuscia, Via S.C. DeLellis snc, 01100, Viterbo, Italy. Tree and Timber Institute, National Research Council of Italy, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Italy.
Dugo L
Department of Medicine, University Campus Bio-Medico of Rome, Via Álvaro del Portillo 21, 00128, Rome, Italy.
De Gara L
Department of Medicine, University Campus Bio-Medico of Rome, Via Álvaro del Portillo 21, 00128, Rome, Italy.
Mondello L
Department of Medicine, University Campus Bio-Medico of Rome, Via Álvaro del Portillo 21, 00128, Rome, Italy. Chemistry, Biological, Pharmaceutical and Environmental Sciences Department, University of Messina, Polo Annunziata, Viale Annunziata, 98168, Messina, Italy. Chromaleont s.r.l., Viale Boccetta 70, 98122, Messina, Italy.

MeSH

AnthocyaninsAntioxidantsChromatography, High Pressure LiquidFlavonoidsFood AnalysisFruitFruit and Vegetable JuicesHydrolyzable TanninsLythraceaePhenolsSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29350256

Citation

Russo, Marina, et al. "Analysis of Phenolic Compounds in Different Parts of Pomegranate (Punica Granatum) Fruit By HPLC-PDA-ESI/MS and Evaluation of Their Antioxidant Activity: Application to Different Italian Varieties." Analytical and Bioanalytical Chemistry, vol. 410, no. 15, 2018, pp. 3507-3520.
Russo M, Fanali C, Tripodo G, et al. Analysis of phenolic compounds in different parts of pomegranate (Punica granatum) fruit by HPLC-PDA-ESI/MS and evaluation of their antioxidant activity: application to different Italian varieties. Anal Bioanal Chem. 2018;410(15):3507-3520.
Russo, M., Fanali, C., Tripodo, G., Dugo, P., Muleo, R., Dugo, L., De Gara, L., & Mondello, L. (2018). Analysis of phenolic compounds in different parts of pomegranate (Punica granatum) fruit by HPLC-PDA-ESI/MS and evaluation of their antioxidant activity: application to different Italian varieties. Analytical and Bioanalytical Chemistry, 410(15), 3507-3520. https://doi.org/10.1007/s00216-018-0854-8
Russo M, et al. Analysis of Phenolic Compounds in Different Parts of Pomegranate (Punica Granatum) Fruit By HPLC-PDA-ESI/MS and Evaluation of Their Antioxidant Activity: Application to Different Italian Varieties. Anal Bioanal Chem. 2018;410(15):3507-3520. PubMed PMID: 29350256.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of phenolic compounds in different parts of pomegranate (Punica granatum) fruit by HPLC-PDA-ESI/MS and evaluation of their antioxidant activity: application to different Italian varieties. AU - Russo,Marina, AU - Fanali,Chiara, AU - Tripodo,Giusy, AU - Dugo,Paola, AU - Muleo,Rosario, AU - Dugo,Laura, AU - De Gara,Laura, AU - Mondello,Luigi, Y1 - 2018/01/19/ PY - 2017/10/09/received PY - 2018/01/04/accepted PY - 2017/12/02/revised PY - 2018/1/20/pubmed PY - 2018/6/23/medline PY - 2018/1/20/entrez KW - By-products KW - High-performance liquid chromatography coupled with photodiode array and electrospray ionization mass spectrometry detection KW - Phenolic compounds KW - Pomegranate KW - Preparative liquid chromatography SP - 3507 EP - 3520 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 410 IS - 15 N2 - The analysis of pomegranate phenolic compounds belonging to different classes in different fruit parts was performed by high-performance liquid chromatography coupled with photodiode array and mass spectrometry detection. Two different separation methods were optimized for the analysis of anthocyanins and hydrolyzable tannins along with phenolic acids and flavonoids. Two C18 columns, core-shell and fully porous particle stationary phases, were used. The parameters for separation of phenolic compounds were optimized considering chromatographic resolution and analysis time. Thirty-five phenolic compounds were found, and 28 of them were tentatively identified as belonging to four different phenolic compound classes; namely, anthocyanins, phenolic acids, hydrolyzable tannins, and flavonoids. Quantitative analysis was performed with a mixture of nine phenolic compounds belonging to phenolic compound classes representative of pomegranate. The method was then fully validated in terms of retention time precision, expressed as the relative standard deviation, limit of detection, limit of quantification, and linearity range. Phenolic compounds were analyzed directly in pomegranate juice, and after solvent extraction with a mixture of water and methanol with a small percentage of acid in peel and pulp samples. The accuracy of the extraction method was also assessed, and satisfactory values were obtained. Finally, the method was used to study identified analytes in pomegranate juice, peel, and pulp of six different Italian varieties and one international variety. Differences in phenolic compound profiles among the different pomegranate parts were observed. Pomegranate peel samples showed a high concentration of phenolic compounds, ellagitannins being the most abundant ones, with respect to pulp and juice samples for each variety. With the same samples, total phenols and antioxidant activity were evaluated through colorimetric assays, and the results were correlated among them. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/29350256/Analysis_of_phenolic_compounds_in_different_parts_of_pomegranate__Punica_granatum__fruit_by_HPLC_PDA_ESI/MS_and_evaluation_of_their_antioxidant_activity:_application_to_different_Italian_varieties_ DB - PRIME DP - Unbound Medicine ER -