Tags

Type your tag names separated by a space and hit enter

B Type and Complex A/B Type Epicatechin Trimers Isolated from Litchi pericarp Aqueous Extract Show High Antioxidant and Anticancer Activity.
Int J Mol Sci. 2018 Jan 19; 19(1)IJ

Abstract

Litchi (Litchi chinensis Sonn.) fruit is known for its rich source of phenolics. Litchi pericarp contains high levels of epicatechin that may form oligomers of various lengths. Except for several A or B type epicatechin dimers, other soluble oligomers have rarely been identified in the pericarp. Here, bioassay-guided column fractionation was applied to isolate bioactive phenolics from aqueous pericarp extract. A fraction (S3) was obtained by two rounds of Sephadex LH-20 column chromatography, and showed higher antioxidant activity and inhibition on the proliferation of human lung cancer cells (A549) than Litchi anthocyanins. S3 was further separated to isolate fractions P1-P4, which all showed higher antioxidant activity than vitamin C. P3 showed 32.9% inhibition on A549 cells at 30 μg/mL, higher than other fractions and cis-Dichlorodiamineplatinum (DDP, 0.5 μg/mL), but not as high as the combination of the four fractions. Using HPLC-Q-TOF-MS/MS, one B-type and complex A/B type epicatechin trimers were identified in P3; another B-type and two A/B-type trimers were identified in P4. P1 and P2, containing epicatechin and proanthocyanidin B2, respectively, showed no cell inhibition at 30 μg/mL. It is the first time that the two B type trimers of epicatechins (Litchitannin B1 and B2), have been found in Litchi species. The identified proanthocyanidins were detected in the pericarp of the young fruit, and the levels of the compounds decreased as the fruit developed, correlating to the decreasing patterns of the expression of LcLAR and LcANR, two key genes in the catechin biosynthesis pathway.

Authors+Show Affiliations

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/College of Horticulture, South China Agricultural University, Guangzhou 510642, China. gyhzgh@163.com.College of Life Science, South China Agricultural University, Guangzhou 510642, China. fangfangdc@gmail.com. Research Institute of Food Science and Engineering Technology, Hezhou University Hezhou 542899, China. fangfangdc@gmail.com.College of Life Science, South China Agricultural University, Guangzhou 510642, China. xuelianzhang@scau.edu.cn.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/College of Horticulture, South China Agricultural University, Guangzhou 510642, China. bin278083@foxmail.com.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/College of Horticulture, South China Agricultural University, Guangzhou 510642, China. hhluo@stu.scau.edu.cn.Institute of Biomedicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine, Biomedical Translational Research Institute, Jinan University, Guangzhou 510632, China. 810leezhen@sina.com.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/College of Horticulture, South China Agricultural University, Guangzhou 510642, China. zhang_ivy@163.com. College of Life Science, South China Agricultural University, Guangzhou 510642, China. zhang_ivy@163.com.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/College of Horticulture, South China Agricultural University, Guangzhou 510642, China. zqzhang@scau.edu.cn.College of Life Science, South China Agricultural University, Guangzhou 510642, China. xqpang@scau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29351247

Citation

Gong, Yihui, et al. "B Type and Complex A/B Type Epicatechin Trimers Isolated From Litchi Pericarp Aqueous Extract Show High Antioxidant and Anticancer Activity." International Journal of Molecular Sciences, vol. 19, no. 1, 2018.
Gong Y, Fang F, Zhang X, et al. B Type and Complex A/B Type Epicatechin Trimers Isolated from Litchi pericarp Aqueous Extract Show High Antioxidant and Anticancer Activity. Int J Mol Sci. 2018;19(1).
Gong, Y., Fang, F., Zhang, X., Liu, B., Luo, H., Li, Z., Zhang, X., Zhang, Z., & Pang, X. (2018). B Type and Complex A/B Type Epicatechin Trimers Isolated from Litchi pericarp Aqueous Extract Show High Antioxidant and Anticancer Activity. International Journal of Molecular Sciences, 19(1). https://doi.org/10.3390/ijms19010301
Gong Y, et al. B Type and Complex A/B Type Epicatechin Trimers Isolated From Litchi Pericarp Aqueous Extract Show High Antioxidant and Anticancer Activity. Int J Mol Sci. 2018 Jan 19;19(1) PubMed PMID: 29351247.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - B Type and Complex A/B Type Epicatechin Trimers Isolated from Litchi pericarp Aqueous Extract Show High Antioxidant and Anticancer Activity. AU - Gong,Yihui, AU - Fang,Fang, AU - Zhang,Xin, AU - Liu,Bin, AU - Luo,Honghui, AU - Li,Zhen, AU - Zhang,Xuelian, AU - Zhang,Zhaoqi, AU - Pang,Xuequn, Y1 - 2018/01/19/ PY - 2017/12/31/received PY - 2018/01/12/revised PY - 2018/01/13/accepted PY - 2018/1/20/entrez PY - 2018/1/20/pubmed PY - 2018/8/14/medline KW - A/B-type epicatechin trimers KW - B-type epicatechin trimers KW - Litchi fruit KW - anticancer activity KW - aqueous pericarp extract JF - International journal of molecular sciences JO - Int J Mol Sci VL - 19 IS - 1 N2 - Litchi (Litchi chinensis Sonn.) fruit is known for its rich source of phenolics. Litchi pericarp contains high levels of epicatechin that may form oligomers of various lengths. Except for several A or B type epicatechin dimers, other soluble oligomers have rarely been identified in the pericarp. Here, bioassay-guided column fractionation was applied to isolate bioactive phenolics from aqueous pericarp extract. A fraction (S3) was obtained by two rounds of Sephadex LH-20 column chromatography, and showed higher antioxidant activity and inhibition on the proliferation of human lung cancer cells (A549) than Litchi anthocyanins. S3 was further separated to isolate fractions P1-P4, which all showed higher antioxidant activity than vitamin C. P3 showed 32.9% inhibition on A549 cells at 30 μg/mL, higher than other fractions and cis-Dichlorodiamineplatinum (DDP, 0.5 μg/mL), but not as high as the combination of the four fractions. Using HPLC-Q-TOF-MS/MS, one B-type and complex A/B type epicatechin trimers were identified in P3; another B-type and two A/B-type trimers were identified in P4. P1 and P2, containing epicatechin and proanthocyanidin B2, respectively, showed no cell inhibition at 30 μg/mL. It is the first time that the two B type trimers of epicatechins (Litchitannin B1 and B2), have been found in Litchi species. The identified proanthocyanidins were detected in the pericarp of the young fruit, and the levels of the compounds decreased as the fruit developed, correlating to the decreasing patterns of the expression of LcLAR and LcANR, two key genes in the catechin biosynthesis pathway. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/29351247/B_Type_and_Complex_A/B_Type_Epicatechin_Trimers_Isolated_from_Litchi_pericarp_Aqueous_Extract_Show_High_Antioxidant_and_Anticancer_Activity_ DB - PRIME DP - Unbound Medicine ER -