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Chrysin, a natural and biologically active flavonoid suppresses tumor growth of mouse B16F10 melanoma cells: In vitro and In vivo study.
Chem Biol Interact. 2018 Mar 01; 283:10-19.CB

Abstract

Chrysin (5,7-dihydroxyflavone) is a natural and biologically active compound which has many biological activities as an anticancer agent. The current report is aimed at finding out whether the antitumor potential of chrysin, evidenced in vitro and in vivo, is linked or not to its effect on immunological mechanisms of melanoma-bearing mice. Chrysin-treated B16F10 cells were analyzed for their metabolic rate and apoptotic potentials. In vivo, BALB/c mice received a subcutaneous injection of B16F10 melanoma cells prior to antitumor treatments with chrysin (50 mg/kg b.w) for 14 days and 21 days. The results showed that chrysin inhibited cancer cell growth at a dose-dependent manner by inducing apoptosis and cell cycle arrest at G2/M phase. Moreover, chrysin suppressed melanoma tumor growth at an average of 60% (after 14 days of treatment) and 71% (after 21 days of treatment) compared to the tumor-bearing group. Furthermore, chrysin treatment increased the cytotoxic activity of NK, CTL and macrophages. The findings showed that chrysin antitumor action on the murine melanoma model was very promising, suggesting that chrysin could be a potentially good candidate for future use in alternative anti-melanoma treatments.

Authors+Show Affiliations

Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia; Unity of Bioactive Natural Substances and Biotechnology, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia.Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia; Unity of Bioactive Natural Substances and Biotechnology, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia.Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia; Unity of Bioactive Natural Substances and Biotechnology, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia; University of Tunis-El Manar, Faculty of Sciences of Tunis, Tunisia.Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia; Unity of Bioactive Natural Substances and Biotechnology, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia.Regional Blood Transfusion Centre, CHU Farhat Hached, Sousse, Tunisia.Regional Blood Transfusion Centre, CHU Farhat Hached, Sousse, Tunisia.Unity of Bioactive Natural Substances and Biotechnology, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia.Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia; Unity of Bioactive Natural Substances and Biotechnology, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia. Electronic address: leila.chekir@laposte.net.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29352974

Citation

Sassi, Aïcha, et al. "Chrysin, a Natural and Biologically Active Flavonoid Suppresses Tumor Growth of Mouse B16F10 Melanoma Cells: in Vitro and in Vivo Study." Chemico-biological Interactions, vol. 283, 2018, pp. 10-19.
Sassi A, Maatouk M, El Gueder D, et al. Chrysin, a natural and biologically active flavonoid suppresses tumor growth of mouse B16F10 melanoma cells: In vitro and In vivo study. Chem Biol Interact. 2018;283:10-19.
Sassi, A., Maatouk, M., El Gueder, D., Bzéouich, I. M., Abdelkefi-Ben Hatira, S., Jemni-Yacoub, S., Ghedira, K., & Chekir-Ghedira, L. (2018). Chrysin, a natural and biologically active flavonoid suppresses tumor growth of mouse B16F10 melanoma cells: In vitro and In vivo study. Chemico-biological Interactions, 283, 10-19. https://doi.org/10.1016/j.cbi.2017.11.022
Sassi A, et al. Chrysin, a Natural and Biologically Active Flavonoid Suppresses Tumor Growth of Mouse B16F10 Melanoma Cells: in Vitro and in Vivo Study. Chem Biol Interact. 2018 Mar 1;283:10-19. PubMed PMID: 29352974.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chrysin, a natural and biologically active flavonoid suppresses tumor growth of mouse B16F10 melanoma cells: In vitro and In vivo study. AU - Sassi,Aïcha, AU - Maatouk,Mouna, AU - El Gueder,Dorra, AU - Bzéouich,Imen Mokdad, AU - Abdelkefi-Ben Hatira,Saïda, AU - Jemni-Yacoub,Saloua, AU - Ghedira,Kamel, AU - Chekir-Ghedira,Leila, Y1 - 2018/01/17/ PY - 2017/06/22/received PY - 2017/11/15/revised PY - 2017/11/26/accepted PY - 2018/1/22/pubmed PY - 2018/3/6/medline PY - 2018/1/22/entrez KW - Acridine Orange (PubChem CID: 62344) KW - Ammonium Chloride (PubChem CID: 25517) KW - Anti-tumor activity KW - Apoptosis KW - B16F10 murine melanoma cells KW - Cell cycle KW - Chrysin KW - Chrysin (PubChem CID: 5281607) KW - Dimethyl sulfoxide (PubChem CID: 679) KW - Ethidium Bromide (PubChem CID: 14710) KW - MTT (PubChem CID: 16218671) KW - NK cell cytotoxicity KW - Naphthylethylenediamine dihydrochloride (PubChem CID: 15106) KW - Nitric Oxide (PubChem CID: 145068) KW - Phosphoric acid (PubChem CID:1004) KW - Propidium iodide (PubChem CID: 104981) KW - Ribonuclease A (PubChem CID: 9369) KW - Sulfanilamide (PubChem CID: 5333) KW - p-Nitrophenyl phosphate (PubChem CID: 2723710) SP - 10 EP - 19 JF - Chemico-biological interactions JO - Chem. Biol. Interact. VL - 283 N2 - Chrysin (5,7-dihydroxyflavone) is a natural and biologically active compound which has many biological activities as an anticancer agent. The current report is aimed at finding out whether the antitumor potential of chrysin, evidenced in vitro and in vivo, is linked or not to its effect on immunological mechanisms of melanoma-bearing mice. Chrysin-treated B16F10 cells were analyzed for their metabolic rate and apoptotic potentials. In vivo, BALB/c mice received a subcutaneous injection of B16F10 melanoma cells prior to antitumor treatments with chrysin (50 mg/kg b.w) for 14 days and 21 days. The results showed that chrysin inhibited cancer cell growth at a dose-dependent manner by inducing apoptosis and cell cycle arrest at G2/M phase. Moreover, chrysin suppressed melanoma tumor growth at an average of 60% (after 14 days of treatment) and 71% (after 21 days of treatment) compared to the tumor-bearing group. Furthermore, chrysin treatment increased the cytotoxic activity of NK, CTL and macrophages. The findings showed that chrysin antitumor action on the murine melanoma model was very promising, suggesting that chrysin could be a potentially good candidate for future use in alternative anti-melanoma treatments. SN - 1872-7786 UR - https://www.unboundmedicine.com/medline/citation/29352974/Chrysin_a_natural_and_biologically_active_flavonoid_suppresses_tumor_growth_of_mouse_B16F10_melanoma_cells:_In_vitro_and_In_vivo_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-2797(17)30676-2 DB - PRIME DP - Unbound Medicine ER -