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Development of Thinopyrum ponticum-specific molecular markers and FISH probes based on SLAF-seq technology.
Planta. 2018 May; 247(5):1099-1108.P

Abstract

MAIN CONCLUSION

Based on SLAF-seq, 67 Thinopyrum ponticum-specific markers and eight Th. ponticum-specific FISH probes were developed, and these markers and probes could be used for detection of alien chromatin in a wheat background. Decaploid Thinopyrum ponticum (2n = 10x = 70) is a valuable gene reservoir for wheat improvement. Identification of Th. ponticum introgression would facilitate its transfer into diverse wheat genetic backgrounds and its practical utilization in wheat improvement. Based on specific-locus-amplified fragment sequencing (SLAF-seq) technology, 67 new Th. ponticum-specific molecular markers and eight Th. ponticum-specific fluorescence in situ hybridization (FISH) probes have been developed from a tiny wheat-Th. ponticum translocation line. These newly developed molecular markers allowed the detection of Th. ponticum DNA in a variety of materials specifically and steadily at high throughput. According to the hybridization signal pattern, the eight Th. ponticum-specific probes could be divided into two groups. The first group including five dispersed repetitive sequence probes could identify Th. ponticum chromatin more sensitively and accurately than genomic in situ hybridization (GISH). Whereas the second group having three tandem repetitive sequence probes enabled the discrimination of Th. ponticum chromosomes together with another clone pAs1 in wheat-Th. ponticum partial amphiploid Xiaoyan 68.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering, Beijing, 100101, China.

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering, Beijing, 100101, China. University of Chinese Academy of Sciences, Beijing, 100049, China.

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering, Beijing, 100101, China.

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering, Beijing, 100101, China.

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering, Beijing, 100101, China.

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering, Beijing, 100101, China.

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering, Beijing, 100101, China. zsli@genetics.ac.cn.

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering, Beijing, 100101, China. qzheng@genetics.ac.cn.

MeSH

ChromatinDNA, PlantGenetic MarkersIn Situ Hybridization, FluorescenceNucleic Acid Amplification TechniquesPoaceaeSequence Analysis, DNATriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29356894

Citation

Liu, Liqin, et al. "Development of Thinopyrum Ponticum-specific Molecular Markers and FISH Probes Based On SLAF-seq Technology." Planta, vol. 247, no. 5, 2018, pp. 1099-1108.

Liu L, Luo Q, Teng W, et al. Development of Thinopyrum ponticum-specific molecular markers and FISH probes based on SLAF-seq technology. Planta. 2018;247(5):1099-1108.

Liu, L., Luo, Q., Teng, W., Li, B., Li, H., Li, Y., Li, Z., & Zheng, Q. (2018). Development of Thinopyrum ponticum-specific molecular markers and FISH probes based on SLAF-seq technology. Planta, 247(5), 1099-1108. https://doi.org/10.1007/s00425-018-2845-6

Liu L, et al. Development of Thinopyrum Ponticum-specific Molecular Markers and FISH Probes Based On SLAF-seq Technology. Planta. 2018;247(5):1099-1108. PubMed PMID: 29356894.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Development of Thinopyrum ponticum-specific molecular markers and FISH probes based on SLAF-seq technology. AU - Liu,Liqin, AU - Luo,Qiaoling, AU - Teng,Wan, AU - Li,Bin, AU - Li,Hongwei, AU - Li,Yiwen, AU - Li,Zhensheng, AU - Zheng,Qi, Y1 - 2018/01/22/ PY - 2017/10/17/received PY - 2018/01/07/accepted PY - 2018/1/23/pubmed PY - 2018/8/22/medline PY - 2018/1/23/entrez KW - FISH KW - GISH KW - Molecular markers KW - SLAF-seq KW - Thinopyrum ponticum KW - Triticum aestivum SP - 1099 EP - 1108 JF - Planta JO - Planta VL - 247 IS - 5 N2 - MAIN CONCLUSION: Based on SLAF-seq, 67 Thinopyrum ponticum-specific markers and eight Th. ponticum-specific FISH probes were developed, and these markers and probes could be used for detection of alien chromatin in a wheat background. Decaploid Thinopyrum ponticum (2n = 10x = 70) is a valuable gene reservoir for wheat improvement. Identification of Th. ponticum introgression would facilitate its transfer into diverse wheat genetic backgrounds and its practical utilization in wheat improvement. Based on specific-locus-amplified fragment sequencing (SLAF-seq) technology, 67 new Th. ponticum-specific molecular markers and eight Th. ponticum-specific fluorescence in situ hybridization (FISH) probes have been developed from a tiny wheat-Th. ponticum translocation line. These newly developed molecular markers allowed the detection of Th. ponticum DNA in a variety of materials specifically and steadily at high throughput. According to the hybridization signal pattern, the eight Th. ponticum-specific probes could be divided into two groups. The first group including five dispersed repetitive sequence probes could identify Th. ponticum chromatin more sensitively and accurately than genomic in situ hybridization (GISH). Whereas the second group having three tandem repetitive sequence probes enabled the discrimination of Th. ponticum chromosomes together with another clone pAs1 in wheat-Th. ponticum partial amphiploid Xiaoyan 68. SN - 1432-2048 UR - https://www.unboundmedicine.com/medline/citation/29356894/Development_of_Thinopyrum_ponticum_specific_molecular_markers_and_FISH_probes_based_on_SLAF_seq_technology_ DB - PRIME DP - Unbound Medicine ER -