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Growth plate-derived hedgehog-signal-responsive cells provide skeletal tissue components in growing bone.
Histochem Cell Biol. 2018 Apr; 149(4):365-373.HC

Abstract

Longitudinal bone growth progresses by continuous bone replacement of epiphyseal cartilaginous tissue, known as "growth plate", produced by columnar proliferated- and differentiated-epiphyseal chondrocytes. The endochondral ossification process at the growth plate is governed by paracrine signals secreted from terminally differentiated chondrocytes (hypertrophic chondrocytes), and hedgehog signaling is one of the best known regulatory signaling pathways in this process. Here, to investigate the developmental relationship between longitudinal endochondral bone formation and osteogenic progenitors under the influence of hedgehog signaling at the growth plate, genetic lineage tracing was carried out with the use of Gli1CreERT2 mice line to follow the fate of hedgehog-signal-responsive cells during endochondral bone formation. Gli1CreERT2 genetically labeled cells are detected in hypertrophic chondrocytes and osteo-progenitors at the chondro-osseous junction (COJ); these progeny then commit to the osteogenic lineage in periosteum, trabecular and cortical bone along the developing longitudinal axis. Furthermore, in ageing bone, where longitudinal bone growth ceases, hedgehog-signal responsiveness and its implication in osteogenic lineage commitment is significantly weakened. These results show, for the first time, evidence of the developmental contribution of endochondral progenitors under the influence of epiphyseal chondrocyte-derived secretory signals in longitudinally growing bone. This study provides a precise outline for assessing the skeletal lineage commitment of osteo-progenitors in response to growth-plate-derived regulatory signals during endochondral bone formation.

Authors+Show Affiliations

Department of Molecular Pathology, Ehime University Graduate School of Medicine, Shitsukawa, Toon City, Ehime, 791-0295, Japan. ryumaha@m.ehime-u.ac.jp.Department of Molecular Pathology, Ehime University Graduate School of Medicine, Shitsukawa, Toon City, Ehime, 791-0295, Japan. Department of Diagnostic Pathology, Ehime University Hospital, Shitsukawa, Toon City, Ehime, 791-0295, Japan.Division of Integrative Pathophysiology, Proteo-Science Center, Ehime University Graduate School of Medicine, Shitsukawa, Toon City, Ehime, 791-0295, Japan.Department of Molecular Pathology, Ehime University Graduate School of Medicine, Shitsukawa, Toon City, Ehime, 791-0295, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29356962

Citation

Haraguchi, Ryuma, et al. "Growth Plate-derived Hedgehog-signal-responsive Cells Provide Skeletal Tissue Components in Growing Bone." Histochemistry and Cell Biology, vol. 149, no. 4, 2018, pp. 365-373.
Haraguchi R, Kitazawa R, Imai Y, et al. Growth plate-derived hedgehog-signal-responsive cells provide skeletal tissue components in growing bone. Histochem Cell Biol. 2018;149(4):365-373.
Haraguchi, R., Kitazawa, R., Imai, Y., & Kitazawa, S. (2018). Growth plate-derived hedgehog-signal-responsive cells provide skeletal tissue components in growing bone. Histochemistry and Cell Biology, 149(4), 365-373. https://doi.org/10.1007/s00418-018-1641-5
Haraguchi R, et al. Growth Plate-derived Hedgehog-signal-responsive Cells Provide Skeletal Tissue Components in Growing Bone. Histochem Cell Biol. 2018;149(4):365-373. PubMed PMID: 29356962.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Growth plate-derived hedgehog-signal-responsive cells provide skeletal tissue components in growing bone. AU - Haraguchi,Ryuma, AU - Kitazawa,Riko, AU - Imai,Yuuki, AU - Kitazawa,Sohei, Y1 - 2018/01/22/ PY - 2018/01/18/accepted PY - 2018/1/23/pubmed PY - 2018/5/17/medline PY - 2018/1/23/entrez KW - Endochondral bone development KW - Genetic lineage tracing KW - Growth plate KW - Hedgehog signaling SP - 365 EP - 373 JF - Histochemistry and cell biology JO - Histochem Cell Biol VL - 149 IS - 4 N2 - Longitudinal bone growth progresses by continuous bone replacement of epiphyseal cartilaginous tissue, known as "growth plate", produced by columnar proliferated- and differentiated-epiphyseal chondrocytes. The endochondral ossification process at the growth plate is governed by paracrine signals secreted from terminally differentiated chondrocytes (hypertrophic chondrocytes), and hedgehog signaling is one of the best known regulatory signaling pathways in this process. Here, to investigate the developmental relationship between longitudinal endochondral bone formation and osteogenic progenitors under the influence of hedgehog signaling at the growth plate, genetic lineage tracing was carried out with the use of Gli1CreERT2 mice line to follow the fate of hedgehog-signal-responsive cells during endochondral bone formation. Gli1CreERT2 genetically labeled cells are detected in hypertrophic chondrocytes and osteo-progenitors at the chondro-osseous junction (COJ); these progeny then commit to the osteogenic lineage in periosteum, trabecular and cortical bone along the developing longitudinal axis. Furthermore, in ageing bone, where longitudinal bone growth ceases, hedgehog-signal responsiveness and its implication in osteogenic lineage commitment is significantly weakened. These results show, for the first time, evidence of the developmental contribution of endochondral progenitors under the influence of epiphyseal chondrocyte-derived secretory signals in longitudinally growing bone. This study provides a precise outline for assessing the skeletal lineage commitment of osteo-progenitors in response to growth-plate-derived regulatory signals during endochondral bone formation. SN - 1432-119X UR - https://www.unboundmedicine.com/medline/citation/29356962/Growth_plate_derived_hedgehog_signal_responsive_cells_provide_skeletal_tissue_components_in_growing_bone_ L2 - https://dx.doi.org/10.1007/s00418-018-1641-5 DB - PRIME DP - Unbound Medicine ER -