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Detection and Quantification of Cannabinoids in Extracts of Cannabis sativa Roots Using LC-MS/MS.
Planta Med. 2018 Mar; 84(4):267-271.PM

Abstract

A liquid chromatography-tandem mass spectrometry single-laboratory validation was performed for the detection and quantification of the 10 major cannabinoids of cannabis, namely, (-)-trans-Δ9-tetrahydrocannabinol, cannabidiol, cannabigerol, cannabichromene, tetrahydrocannabivarian, cannabinol, (-)-trans-Δ8-tetrahydrocannabinol, cannabidiolic acid, cannabigerolic acid, and Δ9-tetrahydrocannabinolic acid-A, in the root extract of Cannabis sativa. Acetonitrile : methanol (80 : 20, v/v) was used for extraction; d3-cannabidiol and d3- tetrahydrocannabinol were used as the internal standards. All 10 cannabinoids showed a good regression relationship with r2 > 0.99. The validated method is simple, sensitive, and reproducible and is therefore suitable for the detection and quantification of these cannabinoids in extracts of cannabis roots. To our knowledge, this is the first report for the quantification of cannabinoids in cannabis roots.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Gul W
ElSohly Laboratories, Inc., Oxford, Mississippi, United States of America. National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, Mississippi, United States of America.
Gul SW
ElSohly Laboratories, Inc., Oxford, Mississippi, United States of America.
Chandra S
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, Mississippi, United States of America.
Lata H
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, Mississippi, United States of America.
Ibrahim EA
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, Mississippi, United States of America. Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
ElSohly MA
ElSohly Laboratories, Inc., Oxford, Mississippi, United States of America. National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, Mississippi, United States of America. Department of Pharmaceutics and Drug Delivery, School of Pharmacy, University of Mississippi, University, Mississippi, United States of America.

MeSH

CannabinoidsCannabisChromatography, LiquidPlant ExtractsPlant RootsTandem Mass Spectrometry

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29359294

Citation

Gul, Waseem, et al. "Detection and Quantification of Cannabinoids in Extracts of Cannabis Sativa Roots Using LC-MS/MS." Planta Medica, vol. 84, no. 4, 2018, pp. 267-271.
Gul W, Gul SW, Chandra S, et al. Detection and Quantification of Cannabinoids in Extracts of Cannabis sativa Roots Using LC-MS/MS. Planta Med. 2018;84(4):267-271.
Gul, W., Gul, S. W., Chandra, S., Lata, H., Ibrahim, E. A., & ElSohly, M. A. (2018). Detection and Quantification of Cannabinoids in Extracts of Cannabis sativa Roots Using LC-MS/MS. Planta Medica, 84(4), 267-271. https://doi.org/10.1055/s-0044-100798
Gul W, et al. Detection and Quantification of Cannabinoids in Extracts of Cannabis Sativa Roots Using LC-MS/MS. Planta Med. 2018;84(4):267-271. PubMed PMID: 29359294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection and Quantification of Cannabinoids in Extracts of Cannabis sativa Roots Using LC-MS/MS. AU - Gul,Waseem, AU - Gul,Shahbaz W, AU - Chandra,Suman, AU - Lata,Hemant, AU - Ibrahim,Elsayed A, AU - ElSohly,Mahmoud A, Y1 - 2018/01/22/ PY - 2018/1/24/pubmed PY - 2018/8/10/medline PY - 2018/1/24/entrez SP - 267 EP - 271 JF - Planta medica JO - Planta Med VL - 84 IS - 4 N2 - A liquid chromatography-tandem mass spectrometry single-laboratory validation was performed for the detection and quantification of the 10 major cannabinoids of cannabis, namely, (-)-trans-Δ9-tetrahydrocannabinol, cannabidiol, cannabigerol, cannabichromene, tetrahydrocannabivarian, cannabinol, (-)-trans-Δ8-tetrahydrocannabinol, cannabidiolic acid, cannabigerolic acid, and Δ9-tetrahydrocannabinolic acid-A, in the root extract of Cannabis sativa. Acetonitrile : methanol (80 : 20, v/v) was used for extraction; d3-cannabidiol and d3- tetrahydrocannabinol were used as the internal standards. All 10 cannabinoids showed a good regression relationship with r2 > 0.99. The validated method is simple, sensitive, and reproducible and is therefore suitable for the detection and quantification of these cannabinoids in extracts of cannabis roots. To our knowledge, this is the first report for the quantification of cannabinoids in cannabis roots. SN - 1439-0221 UR - https://www.unboundmedicine.com/medline/citation/29359294/Detection_and_Quantification_of_Cannabinoids_in_Extracts_of_Cannabis_sativa_Roots_Using_LC_MS/MS_ DB - PRIME DP - Unbound Medicine ER -