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Site-Specific Incorporation of a Thioester Containing Amino Acid into Proteins.
ACS Chem Biol. 2018 03 16; 13(3):578-581.AC

Abstract

Here, we report the site-specific incorporation of a thioester containing noncanonical amino acid (ncAA) into recombinantly expressed proteins. Specifically, we genetically encoded a thioester-activated aspartic acid (ThioD) in bacteria in good yield and with high fidelity using an orthogonal nonsense suppressor tRNA/aminoacyl-tRNA synthetase (aaRS) pair. To demonstrate the utility of ThioD, we used native chemical ligation to label green fluorescent protein with a fluorophore in good yield.

Authors+Show Affiliations

Department of Chemistry and Skaggs Institute for Chemical Biology , The Scripps Research Institute , 10550 N Torrey Pines Road , La Jolla , California 92037 , United States.Department of Chemistry , Washington State University , Pullman , Washington 99164-4630 , United States.Department of Chemistry and Skaggs Institute for Chemical Biology , The Scripps Research Institute , 10550 N Torrey Pines Road , La Jolla , California 92037 , United States.Department of Chemistry and Skaggs Institute for Chemical Biology , The Scripps Research Institute , 10550 N Torrey Pines Road , La Jolla , California 92037 , United States.Department of Chemistry and Skaggs Institute for Chemical Biology , The Scripps Research Institute , 10550 N Torrey Pines Road , La Jolla , California 92037 , United States.Department of Chemistry and Skaggs Institute for Chemical Biology , The Scripps Research Institute , 10550 N Torrey Pines Road , La Jolla , California 92037 , United States.Department of Chemistry , Washington State University , Pullman , Washington 99164-4630 , United States.Department of Chemistry and Skaggs Institute for Chemical Biology , The Scripps Research Institute , 10550 N Torrey Pines Road , La Jolla , California 92037 , United States.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

29360343

Citation

Xuan, Weimin, et al. "Site-Specific Incorporation of a Thioester Containing Amino Acid Into Proteins." ACS Chemical Biology, vol. 13, no. 3, 2018, pp. 578-581.
Xuan W, Collins D, Koh M, et al. Site-Specific Incorporation of a Thioester Containing Amino Acid into Proteins. ACS Chem Biol. 2018;13(3):578-581.
Xuan, W., Collins, D., Koh, M., Shao, S., Yao, A., Xiao, H., Garner, P., & Schultz, P. G. (2018). Site-Specific Incorporation of a Thioester Containing Amino Acid into Proteins. ACS Chemical Biology, 13(3), 578-581. https://doi.org/10.1021/acschembio.7b00998
Xuan W, et al. Site-Specific Incorporation of a Thioester Containing Amino Acid Into Proteins. ACS Chem Biol. 2018 03 16;13(3):578-581. PubMed PMID: 29360343.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Site-Specific Incorporation of a Thioester Containing Amino Acid into Proteins. AU - Xuan,Weimin, AU - Collins,Daniel, AU - Koh,Minseob, AU - Shao,Sida, AU - Yao,Anzhi, AU - Xiao,Han, AU - Garner,Philip, AU - Schultz,Peter G, Y1 - 2018/02/09/ PY - 2018/1/24/pubmed PY - 2019/4/6/medline PY - 2018/1/24/entrez SP - 578 EP - 581 JF - ACS chemical biology JO - ACS Chem. Biol. VL - 13 IS - 3 N2 - Here, we report the site-specific incorporation of a thioester containing noncanonical amino acid (ncAA) into recombinantly expressed proteins. Specifically, we genetically encoded a thioester-activated aspartic acid (ThioD) in bacteria in good yield and with high fidelity using an orthogonal nonsense suppressor tRNA/aminoacyl-tRNA synthetase (aaRS) pair. To demonstrate the utility of ThioD, we used native chemical ligation to label green fluorescent protein with a fluorophore in good yield. SN - 1554-8937 UR - https://www.unboundmedicine.com/medline/citation/29360343/Site_Specific_Incorporation_of_a_Thioester_Containing_Amino_Acid_into_Proteins_ L2 - https://dx.doi.org/10.1021/acschembio.7b00998 DB - PRIME DP - Unbound Medicine ER -