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The GATA transcription factor ELT-2 modulates both the expression and methyltransferase activity of PRMT-1 in Caenorhabditis elegans.
J Biochem. 2018 May 01; 163(5):433-440.JB

Abstract

Protein arginine methyltransferase 1 (PRMT1) catalyzes asymmetric arginine dimethylation of cellular proteins and thus modulates various biological processes, including gene regulation, RNA metabolism, cell signaling and DNA repair. Since prmt-1 null mutant completely abolishes asymmetric dimethylarginine in C. elegans, PRMT-1 is thought to play a crucial role in determining levels of asymmetric arginine dimethylation. However, the mechanism underlying the regulation of PRMT-1 activity remains largely unknown. Here, we explored for transcription factors that induce the expression of PRMT-1 by an RNAi screen using transgenic C. elegans harbouring prmt-1 promoter upstream of gfp. Of 529 clones, we identify a GATA transcription factor elt-2 as a positive regulator of Pprmt-1:: gfp expression and show that elt-2 RNAi decreases endogenous PRMT-1 expression at mRNA and protein levels. Nevertheless, surprisingly arginine methylation levels are increased when elt-2 is silenced, implying that erythroid-like transcription factor (ELT)-2 may also have ability to inhibit methyltransferase activity of PRMT-1. Supporting this idea, GST pull-down and co-immunoprecipitation assays demonstrate the interaction between ELT-2 and PRMT-1. Furthermore, we find that ELT-2 interferes with PRMT-1-induced arginine methylation in a dose-dependent manner. Collectively, our results illustrate the two modes of PRMT-1 regulation, which could determine the levels of asymmetric arginine dimethylation in C. elegans.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Araoi S
Graduate School of Life and Environmental Sciences.
Daitoku H
Life Science Center, Tsukuba Advanced Research Alliance.
Yokoyama A
Graduate School of Life and Environmental Sciences.
Kako K
Faculty of Life and Environmental Sciences.
Hirota K
Faculty of Life and Environmental Sciences. Human Biology, School of Integrative and Global Majors, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8577, Japan.
Fukamizu A
Life Science Center, Tsukuba Advanced Research Alliance.

MeSH

AnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsCells, CulturedGATA Transcription FactorsHEK293 CellsHumansProtein-Arginine N-Methyltransferases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29361115

Citation

Araoi, Sho, et al. "The GATA Transcription Factor ELT-2 Modulates Both the Expression and Methyltransferase Activity of PRMT-1 in Caenorhabditis Elegans." Journal of Biochemistry, vol. 163, no. 5, 2018, pp. 433-440.
Araoi S, Daitoku H, Yokoyama A, et al. The GATA transcription factor ELT-2 modulates both the expression and methyltransferase activity of PRMT-1 in Caenorhabditis elegans. J Biochem. 2018;163(5):433-440.
Araoi, S., Daitoku, H., Yokoyama, A., Kako, K., Hirota, K., & Fukamizu, A. (2018). The GATA transcription factor ELT-2 modulates both the expression and methyltransferase activity of PRMT-1 in Caenorhabditis elegans. Journal of Biochemistry, 163(5), 433-440. https://doi.org/10.1093/jb/mvy012
Araoi S, et al. The GATA Transcription Factor ELT-2 Modulates Both the Expression and Methyltransferase Activity of PRMT-1 in Caenorhabditis Elegans. J Biochem. 2018 May 1;163(5):433-440. PubMed PMID: 29361115.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The GATA transcription factor ELT-2 modulates both the expression and methyltransferase activity of PRMT-1 in Caenorhabditis elegans. AU - Araoi,Sho, AU - Daitoku,Hiroaki, AU - Yokoyama,Atsuko, AU - Kako,Koichiro, AU - Hirota,Keiko, AU - Fukamizu,Akiyoshi, PY - 2017/10/03/received PY - 2017/12/01/accepted PY - 2018/1/24/pubmed PY - 2018/5/16/medline PY - 2018/1/24/entrez SP - 433 EP - 440 JF - Journal of biochemistry JO - J Biochem VL - 163 IS - 5 N2 - Protein arginine methyltransferase 1 (PRMT1) catalyzes asymmetric arginine dimethylation of cellular proteins and thus modulates various biological processes, including gene regulation, RNA metabolism, cell signaling and DNA repair. Since prmt-1 null mutant completely abolishes asymmetric dimethylarginine in C. elegans, PRMT-1 is thought to play a crucial role in determining levels of asymmetric arginine dimethylation. However, the mechanism underlying the regulation of PRMT-1 activity remains largely unknown. Here, we explored for transcription factors that induce the expression of PRMT-1 by an RNAi screen using transgenic C. elegans harbouring prmt-1 promoter upstream of gfp. Of 529 clones, we identify a GATA transcription factor elt-2 as a positive regulator of Pprmt-1:: gfp expression and show that elt-2 RNAi decreases endogenous PRMT-1 expression at mRNA and protein levels. Nevertheless, surprisingly arginine methylation levels are increased when elt-2 is silenced, implying that erythroid-like transcription factor (ELT)-2 may also have ability to inhibit methyltransferase activity of PRMT-1. Supporting this idea, GST pull-down and co-immunoprecipitation assays demonstrate the interaction between ELT-2 and PRMT-1. Furthermore, we find that ELT-2 interferes with PRMT-1-induced arginine methylation in a dose-dependent manner. Collectively, our results illustrate the two modes of PRMT-1 regulation, which could determine the levels of asymmetric arginine dimethylation in C. elegans. SN - 1756-2651 UR - https://www.unboundmedicine.com/medline/citation/29361115/The_GATA_transcription_factor_ELT_2_modulates_both_the_expression_and_methyltransferase_activity_of_PRMT_1_in_Caenorhabditis_elegans_ DB - PRIME DP - Unbound Medicine ER -