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Evaluation of biological potential of selected species of family Poaceae from Bahawalpur, Pakistan.
BMC Complement Altern Med. 2018 Jan 24; 18(1):27.BC

Abstract

BACKGROUND

Oxidative stress as well as bacterial and fungal infections are common source of diseases while plants are source of medication for curative or protective purposes. Hence, aim of study was to compare the pharmacological potential of seven grass species in two different solvents i.e. ethanol and acetone.

METHODS

Preliminary phytochemical tests were done and antioxidant activities were evaluated using ELISA and their IC50 values and AAI (%) were recorded. ANOVA was used for statistical analyses. DNA damage protection assay was done using p1391Z plasmid DNA and DNA bands were analyzed. Antimicrobial activity was done via disc diffusion method and MIC and Activity Index were determined. Cytotoxic activity was carried out using the brine shrimps' assay and LC50 values were calculated using probit analysis program.

RESULTS

Phytochemical studies confirmed the presence of secondary metabolites in most of the plant extracts. Maximum antioxidant potential was revealed in DiAEE, DiAAE (AAI- 54.54% and 43.24%) and DaAEE and DaAAE (AAI- 49.13% and 44.52%). However, PoAEE and PoAAE showed minimum antioxidant potential (AAI- 41.04% and 34.11%). SaSEE, DiAEE and ElIEE showed very little DNA damage protection activity. In antimicrobial assay, DaAEE significantly inhibited the growth of most of the microbial pathogens (nine microbes out of eleven tested microbes) among ethanol extracts while DaAAE and ImCAE showed maximum inhibition (eight microbes out of eleven tested microbes) among acetone plant extracts. However, PoAEE and PoAAE showed least antimicrobial activity. F. oxysporum and A. niger were revealed as the most resistant micro-organisms. ImCEA and ImCAE showed maximum cytotoxic potential (LC50 11.004 ppm and 7.932 ppm) as compared to the other plant extracts.

CONCLUSION

Fodder grasses also contains a substantial phenols and flavonoids contents along with other secondary metabolites and, hence, possess a significant medicinal value. Ethanol extracts showed more therapeutic potential as compared to the acetone extracts. This study provides experimental evidence that the selected species contains such valuable natural compounds which can be used as medicinal drugs in future.

Authors+Show Affiliations

Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.Department of Zoology, University of Gujrat Sub-Campus Rawalpindi, Gujrat, Pakistan.Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan. tmahmood@qau.edu.pk.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29361962

Citation

Fatima, Iram, et al. "Evaluation of Biological Potential of Selected Species of Family Poaceae From Bahawalpur, Pakistan." BMC Complementary and Alternative Medicine, vol. 18, no. 1, 2018, p. 27.
Fatima I, Kanwal S, Mahmood T. Evaluation of biological potential of selected species of family Poaceae from Bahawalpur, Pakistan. BMC Complement Altern Med. 2018;18(1):27.
Fatima, I., Kanwal, S., & Mahmood, T. (2018). Evaluation of biological potential of selected species of family Poaceae from Bahawalpur, Pakistan. BMC Complementary and Alternative Medicine, 18(1), 27. https://doi.org/10.1186/s12906-018-2092-1
Fatima I, Kanwal S, Mahmood T. Evaluation of Biological Potential of Selected Species of Family Poaceae From Bahawalpur, Pakistan. BMC Complement Altern Med. 2018 Jan 24;18(1):27. PubMed PMID: 29361962.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of biological potential of selected species of family Poaceae from Bahawalpur, Pakistan. AU - Fatima,Iram, AU - Kanwal,Sobia, AU - Mahmood,Tariq, Y1 - 2018/01/24/ PY - 2017/08/26/received PY - 2018/01/15/accepted PY - 2018/1/25/entrez PY - 2018/1/25/pubmed PY - 2018/3/6/medline KW - Antibacterial activity KW - Antifungal activity KW - Antioxidant activity KW - Brine shrimps’ Cytotoxicity KW - Fodder grasses KW - Phytochemical analysis KW - p1391Z plasmid DNA SP - 27 EP - 27 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 18 IS - 1 N2 - BACKGROUND: Oxidative stress as well as bacterial and fungal infections are common source of diseases while plants are source of medication for curative or protective purposes. Hence, aim of study was to compare the pharmacological potential of seven grass species in two different solvents i.e. ethanol and acetone. METHODS: Preliminary phytochemical tests were done and antioxidant activities were evaluated using ELISA and their IC50 values and AAI (%) were recorded. ANOVA was used for statistical analyses. DNA damage protection assay was done using p1391Z plasmid DNA and DNA bands were analyzed. Antimicrobial activity was done via disc diffusion method and MIC and Activity Index were determined. Cytotoxic activity was carried out using the brine shrimps' assay and LC50 values were calculated using probit analysis program. RESULTS: Phytochemical studies confirmed the presence of secondary metabolites in most of the plant extracts. Maximum antioxidant potential was revealed in DiAEE, DiAAE (AAI- 54.54% and 43.24%) and DaAEE and DaAAE (AAI- 49.13% and 44.52%). However, PoAEE and PoAAE showed minimum antioxidant potential (AAI- 41.04% and 34.11%). SaSEE, DiAEE and ElIEE showed very little DNA damage protection activity. In antimicrobial assay, DaAEE significantly inhibited the growth of most of the microbial pathogens (nine microbes out of eleven tested microbes) among ethanol extracts while DaAAE and ImCAE showed maximum inhibition (eight microbes out of eleven tested microbes) among acetone plant extracts. However, PoAEE and PoAAE showed least antimicrobial activity. F. oxysporum and A. niger were revealed as the most resistant micro-organisms. ImCEA and ImCAE showed maximum cytotoxic potential (LC50 11.004 ppm and 7.932 ppm) as compared to the other plant extracts. CONCLUSION: Fodder grasses also contains a substantial phenols and flavonoids contents along with other secondary metabolites and, hence, possess a significant medicinal value. Ethanol extracts showed more therapeutic potential as compared to the acetone extracts. This study provides experimental evidence that the selected species contains such valuable natural compounds which can be used as medicinal drugs in future. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/29361962/Evaluation_of_biological_potential_of_selected_species_of_family_Poaceae_from_Bahawalpur_Pakistan_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-018-2092-1 DB - PRIME DP - Unbound Medicine ER -