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Metabolic Profiling of Urine by Capillary Electrophoresis-Mass Spectrometry Using Non-covalently Coated Capillaries.
Methods Mol Biol. 2018; 1730:295-304.MM

Abstract

In the field of metabolomics, capillary electrophoresis-mass spectrometry (CE-MS) can be considered a very useful analytical tool for the profiling of polar and charged metabolites. However, variability of migration time is an important issue in CE. An elegant way to minimize this problem is the use of non-covalently coated capillaries that is dynamic coating of the bare fused-silica capillary with solutions of charged polymers. In this protocol, an improved strategy for the profiling of cationic metabolites in urine by CE-MS using multilayered non-covalent capillary coatings is presented. Capillaries are coated with a bilayer of polybrene (PB) and poly(vinyl sulfonate) (PVS) or with a triple layer of PB, dextran sulfate (DS), and PB. The bilayer- and triple-layer-coated capillaries have a negative and positive outside layer, respectively. It is shown that the use of such capillaries provides very repeatable migration times.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Ramautar R
Division of Systems Biomedicine and Pharmacology, Leiden Academic Center for Drug Research, Leiden University, Leiden, The Netherlands. rawi.ramautar@gmail.com.

MeSH

Dextran SulfateElectrophoresis, CapillaryHexadimethrine BromideHumansMass SpectrometryMetabolomicsPolyvinylsSulfonic AcidsSurface PropertiesUrine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29363083

Citation

Ramautar, Rawi. "Metabolic Profiling of Urine By Capillary Electrophoresis-Mass Spectrometry Using Non-covalently Coated Capillaries." Methods in Molecular Biology (Clifton, N.J.), vol. 1730, 2018, pp. 295-304.
Ramautar R. Metabolic Profiling of Urine by Capillary Electrophoresis-Mass Spectrometry Using Non-covalently Coated Capillaries. Methods Mol Biol. 2018;1730:295-304.
Ramautar, R. (2018). Metabolic Profiling of Urine by Capillary Electrophoresis-Mass Spectrometry Using Non-covalently Coated Capillaries. Methods in Molecular Biology (Clifton, N.J.), 1730, 295-304. https://doi.org/10.1007/978-1-4939-7592-1_22
Ramautar R. Metabolic Profiling of Urine By Capillary Electrophoresis-Mass Spectrometry Using Non-covalently Coated Capillaries. Methods Mol Biol. 2018;1730:295-304. PubMed PMID: 29363083.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic Profiling of Urine by Capillary Electrophoresis-Mass Spectrometry Using Non-covalently Coated Capillaries. A1 - Ramautar,Rawi, PY - 2018/1/25/entrez PY - 2018/1/25/pubmed PY - 2018/12/18/medline KW - Capillary electrophoresis KW - Cationic metabolites KW - Mass spectrometry KW - Metabolomics KW - Non-covalently coated capillaries KW - Urine SP - 295 EP - 304 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 1730 N2 - In the field of metabolomics, capillary electrophoresis-mass spectrometry (CE-MS) can be considered a very useful analytical tool for the profiling of polar and charged metabolites. However, variability of migration time is an important issue in CE. An elegant way to minimize this problem is the use of non-covalently coated capillaries that is dynamic coating of the bare fused-silica capillary with solutions of charged polymers. In this protocol, an improved strategy for the profiling of cationic metabolites in urine by CE-MS using multilayered non-covalent capillary coatings is presented. Capillaries are coated with a bilayer of polybrene (PB) and poly(vinyl sulfonate) (PVS) or with a triple layer of PB, dextran sulfate (DS), and PB. The bilayer- and triple-layer-coated capillaries have a negative and positive outside layer, respectively. It is shown that the use of such capillaries provides very repeatable migration times. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/29363083/Metabolic_Profiling_of_Urine_by_Capillary_Electrophoresis_Mass_Spectrometry_Using_Non_covalently_Coated_Capillaries_ DB - PRIME DP - Unbound Medicine ER -