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Prevalence and Characterization of Staphylococcus aureus Strains in the Pork Chain Supply in Chile.
Foodborne Pathog Dis. 2018 05; 15(5):262-268.FP

Abstract

The detection of methicillin-resistant Staphylococcus aureus (MRSA) and other emerging strains in meat-producing animals and retail meat has increased the risk of contamination of food. The aim of this study was to determine the prevalence and characterize S. aureus strains isolated from the pork chain supply in Chile. A total of 487 samples were collected: 332 samples from pigs at farms and slaughterhouses (nasal, n = 155; skin, n = 177); 85 samples from carcasses at slaughterhouses; and 70 meat samples at supermarkets and retail stores. The isolation of S. aureus was carried out by selective enrichment and culture media. Biochemical testing (API® Staph) and PCR (detection of the nuc and mecA genes) were used to confirm S. aureus and MRSA strains. The agglutination test was used to determine the protein PBP2'. Enterotoxins (SEA, SEB, SEC, SED) were determined by agglutination test and the se genes by PCR method. Oxacillin and cefoxitin susceptibility testing were carried out using the diffusion method. The overall prevalence of S. aureus in the pork meat supply was 33.9%. A higher prevalence was detected on carcasses (56.5%), in pigs sampled at farms (40.6%) than in pigs sampled at slaughterhouses (23.3%) and in nonpackaged retail meat (43.1%) than packaged retail meat (5.3%) (p ≤ 0.05). No significant differences (p > 0.05) were found between the prevalence in pigs (28.3%) and pork meat (32.9%) and between natural pig farming (33.3%) and conventional production (52.8%). The mecA gene and the protein PBP2' were not detected in S. aureus strains. Two S. aureus strains exhibited oxacillin and cefoxitin resistance, and one S. aureus strain was resistant to cefoxitin. One S. aureus strain isolated from a meat sample was positive for enterotoxin SEB. Although the mecA gene was not detected, oxacillin-resistant and seb-producing S. aureus strains were detected, which represent a risk in the pork chain supply.

Authors+Show Affiliations

1 Department of Animal Sciences, Faculty of Agronomy, University of Concepción , Chillán, Chile .1 Department of Animal Sciences, Faculty of Agronomy, University of Concepción , Chillán, Chile .1 Department of Animal Sciences, Faculty of Agronomy, University of Concepción , Chillán, Chile .1 Department of Animal Sciences, Faculty of Agronomy, University of Concepción , Chillán, Chile .1 Department of Animal Sciences, Faculty of Agronomy, University of Concepción , Chillán, Chile .1 Department of Animal Sciences, Faculty of Agronomy, University of Concepción , Chillán, Chile .2 Laboratory of Antimicrobial Agents Research, Department of Microbiology, Faculty of Biological Sciences, University of Concepción , Concepción, Chile .1 Department of Animal Sciences, Faculty of Agronomy, University of Concepción , Chillán, Chile .3 Laboratory of Animal Physiology and Endocrinology, Faculty of Veterinary Sciences, University of Concepción , Chillán, Chile .

Pub Type(s)

Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29364698

Citation

Velasco, Valeria, et al. "Prevalence and Characterization of Staphylococcus Aureus Strains in the Pork Chain Supply in Chile." Foodborne Pathogens and Disease, vol. 15, no. 5, 2018, pp. 262-268.
Velasco V, Vergara JL, Bonilla AM, et al. Prevalence and Characterization of Staphylococcus aureus Strains in the Pork Chain Supply in Chile. Foodborne Pathog Dis. 2018;15(5):262-268.
Velasco, V., Vergara, J. L., Bonilla, A. M., Muñoz, J., Mallea, A., Vallejos, D., Quezada-Aguiluz, M., Campos, J., & Rojas-García, P. (2018). Prevalence and Characterization of Staphylococcus aureus Strains in the Pork Chain Supply in Chile. Foodborne Pathogens and Disease, 15(5), 262-268. https://doi.org/10.1089/fpd.2017.2381
Velasco V, et al. Prevalence and Characterization of Staphylococcus Aureus Strains in the Pork Chain Supply in Chile. Foodborne Pathog Dis. 2018;15(5):262-268. PubMed PMID: 29364698.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence and Characterization of Staphylococcus aureus Strains in the Pork Chain Supply in Chile. AU - Velasco,Valeria, AU - Vergara,José L, AU - Bonilla,Ana M, AU - Muñoz,Javier, AU - Mallea,Alejandra, AU - Vallejos,Diego, AU - Quezada-Aguiluz,Mario, AU - Campos,Jorge, AU - Rojas-García,Pedro, Y1 - 2018/01/24/ PY - 2018/1/25/pubmed PY - 2019/4/16/medline PY - 2018/1/25/entrez KW - Staphylococcus aureus KW - antibiotic susceptibility KW - enterotoxins KW - mecA KW - pigs KW - pork meat SP - 262 EP - 268 JF - Foodborne pathogens and disease JO - Foodborne Pathog Dis VL - 15 IS - 5 N2 - The detection of methicillin-resistant Staphylococcus aureus (MRSA) and other emerging strains in meat-producing animals and retail meat has increased the risk of contamination of food. The aim of this study was to determine the prevalence and characterize S. aureus strains isolated from the pork chain supply in Chile. A total of 487 samples were collected: 332 samples from pigs at farms and slaughterhouses (nasal, n = 155; skin, n = 177); 85 samples from carcasses at slaughterhouses; and 70 meat samples at supermarkets and retail stores. The isolation of S. aureus was carried out by selective enrichment and culture media. Biochemical testing (API® Staph) and PCR (detection of the nuc and mecA genes) were used to confirm S. aureus and MRSA strains. The agglutination test was used to determine the protein PBP2'. Enterotoxins (SEA, SEB, SEC, SED) were determined by agglutination test and the se genes by PCR method. Oxacillin and cefoxitin susceptibility testing were carried out using the diffusion method. The overall prevalence of S. aureus in the pork meat supply was 33.9%. A higher prevalence was detected on carcasses (56.5%), in pigs sampled at farms (40.6%) than in pigs sampled at slaughterhouses (23.3%) and in nonpackaged retail meat (43.1%) than packaged retail meat (5.3%) (p ≤ 0.05). No significant differences (p > 0.05) were found between the prevalence in pigs (28.3%) and pork meat (32.9%) and between natural pig farming (33.3%) and conventional production (52.8%). The mecA gene and the protein PBP2' were not detected in S. aureus strains. Two S. aureus strains exhibited oxacillin and cefoxitin resistance, and one S. aureus strain was resistant to cefoxitin. One S. aureus strain isolated from a meat sample was positive for enterotoxin SEB. Although the mecA gene was not detected, oxacillin-resistant and seb-producing S. aureus strains were detected, which represent a risk in the pork chain supply. SN - 1556-7125 UR - https://www.unboundmedicine.com/medline/citation/29364698/Prevalence_and_Characterization_of_Staphylococcus_aureus_Strains_in_the_Pork_Chain_Supply_in_Chile_ L2 - https://www.liebertpub.com/doi/10.1089/fpd.2017.2381?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -