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Synthesis and Characterisation of Photocrosslinked poly(ethylene glycol) diacrylate Implants for Sustained Ocular Drug Delivery.
Pharm Res. 2018 Jan 16; 35(2):36.PR

Abstract

PURPOSE

To investigate the sustained ocular delivery of small and large drug molecules from photocrosslinked poly(ethylene glycol) diacrylate (PEGDA) implants with varying pore forming agents.

METHODS

Triamcinolone acetonide and ovalbumin loaded photocrosslinked PEGDA implants, with or without pore-forming agents, were fabricated and characterised for chemical, mechanical, swelling, network parameters, as well as drug release and biocompatibility. HPLC-based analytical methods were employed for analysis of two molecules; ELISA was used to demonstrate bioactivity of ovalbumin.

RESULTS

Regardless of PEGDA molecular weight or pore former composition all implants loaded with triamcinolone acetonide released significantly faster than those loaded with ovalbumin. Higher molecular weight PEGDA systems (700 Da) resulted in faster drug release of triamcinolone acetonide than their 250 Da counterpart. All ovalbumin released over the 56-day time period was found to be bioactive. Increasing PEGDA molecular weight resulted in increased system swelling, decreased crosslink density (Ve), increased polymer-water interaction parameter (χ), increased average molecular weight between crosslinks (Mc) and increased mesh size (ε). SEM studies showed the porosity of implants increased with increasing PEGDA molecular weight. Biocompatibility showed both PEGDA molecular weight implants were non-toxic when exposed to retinal epithelial cells over a 7-day period.

CONCLUSION

Photocrosslinked PEGDA implant based systems are capable of controlled drug release of both small and large drug molecules through adaptations in the polymer system network. We are currently continuing evaluation of these systems as potential sustained drug delivery devices.

Authors+Show Affiliations

School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, Belfast, Northern Ireland, BT9 7BL, UK.School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, Belfast, Northern Ireland, BT9 7BL, UK.School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, Belfast, Northern Ireland, BT9 7BL, UK. r.thakur@qub.ac.uk. School of Pharmacy, Medical Biology Centre, Queen's University Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK. r.thakur@qub.ac.uk.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29368249

Citation

McAvoy, Kathryn, et al. "Synthesis and Characterisation of Photocrosslinked Poly(ethylene Glycol) Diacrylate Implants for Sustained Ocular Drug Delivery." Pharmaceutical Research, vol. 35, no. 2, 2018, p. 36.
McAvoy K, Jones D, Thakur RRS. Synthesis and Characterisation of Photocrosslinked poly(ethylene glycol) diacrylate Implants for Sustained Ocular Drug Delivery. Pharm Res. 2018;35(2):36.
McAvoy, K., Jones, D., & Thakur, R. R. S. (2018). Synthesis and Characterisation of Photocrosslinked poly(ethylene glycol) diacrylate Implants for Sustained Ocular Drug Delivery. Pharmaceutical Research, 35(2), 36. https://doi.org/10.1007/s11095-017-2298-9
McAvoy K, Jones D, Thakur RRS. Synthesis and Characterisation of Photocrosslinked Poly(ethylene Glycol) Diacrylate Implants for Sustained Ocular Drug Delivery. Pharm Res. 2018 Jan 16;35(2):36. PubMed PMID: 29368249.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis and Characterisation of Photocrosslinked poly(ethylene glycol) diacrylate Implants for Sustained Ocular Drug Delivery. AU - McAvoy,Kathryn, AU - Jones,David, AU - Thakur,Raghu Raj Singh, Y1 - 2018/01/16/ PY - 2017/09/08/received PY - 2017/11/01/accepted PY - 2018/1/26/entrez PY - 2018/1/26/pubmed PY - 2019/4/30/medline KW - ocular drug delivery KW - photocrosslinked KW - poly(ethylene glycol) diacrylate KW - protein delivery KW - triamcinolone acetonide SP - 36 EP - 36 JF - Pharmaceutical research JO - Pharm. Res. VL - 35 IS - 2 N2 - PURPOSE: To investigate the sustained ocular delivery of small and large drug molecules from photocrosslinked poly(ethylene glycol) diacrylate (PEGDA) implants with varying pore forming agents. METHODS: Triamcinolone acetonide and ovalbumin loaded photocrosslinked PEGDA implants, with or without pore-forming agents, were fabricated and characterised for chemical, mechanical, swelling, network parameters, as well as drug release and biocompatibility. HPLC-based analytical methods were employed for analysis of two molecules; ELISA was used to demonstrate bioactivity of ovalbumin. RESULTS: Regardless of PEGDA molecular weight or pore former composition all implants loaded with triamcinolone acetonide released significantly faster than those loaded with ovalbumin. Higher molecular weight PEGDA systems (700 Da) resulted in faster drug release of triamcinolone acetonide than their 250 Da counterpart. All ovalbumin released over the 56-day time period was found to be bioactive. Increasing PEGDA molecular weight resulted in increased system swelling, decreased crosslink density (Ve), increased polymer-water interaction parameter (χ), increased average molecular weight between crosslinks (Mc) and increased mesh size (ε). SEM studies showed the porosity of implants increased with increasing PEGDA molecular weight. Biocompatibility showed both PEGDA molecular weight implants were non-toxic when exposed to retinal epithelial cells over a 7-day period. CONCLUSION: Photocrosslinked PEGDA implant based systems are capable of controlled drug release of both small and large drug molecules through adaptations in the polymer system network. We are currently continuing evaluation of these systems as potential sustained drug delivery devices. SN - 1573-904X UR - https://www.unboundmedicine.com/medline/citation/29368249/Synthesis_and_Characterisation_of_Photocrosslinked_poly_ethylene_glycol__diacrylate_Implants_for_Sustained_Ocular_Drug_Delivery_ L2 - https://doi.org/10.1007/s11095-017-2298-9 DB - PRIME DP - Unbound Medicine ER -