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Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica.
Virol J. 2018 01 24; 15(1):22.VJ

Abstract

BACKGROUND

Since the discovery of giant viruses infecting amoebae in 2003, many dogmas of virology have been revised and the search for these viruses has been intensified. Over the last few years, several new groups of these viruses have been discovered in various types of samples and environments.In this work, we describe the isolation of 68 giant viruses of amoeba obtained from environmental samples from Brazil and Antarctica.

METHODS

Isolated viruses were identified by hemacolor staining, PCR assays and electron microscopy (scanning and/or transmission).

RESULTS

A total of 64 viruses belonging to the Mimiviridae family were isolated (26 from lineage A, 13 from lineage B, 2 from lineage C and 23 from unidentified lineages) from different types of samples, including marine water from Antarctica, thus being the first mimiviruses isolated in this extreme environment to date. Furthermore, a marseillevirus was isolated from sewage samples along with two pandoraviruses and a cedratvirus (the third to be isolated in the world so far).

CONCLUSIONS

Considering the different type of samples, we found a higher number of viral groups in sewage samples. Our results reinforce the importance of prospective studies in different environmental samples, therefore improving our comprehension about the circulation anddiversity of these viruses in nature.

Authors+Show Affiliations

Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.Laboratório de Diversidade Molecular, Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas, Maceió, Brazil.Departamento de Patologia, Universidade Federal do Espírito Santo, Maruípe, Brazil.Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. Instituto do Petróleo e dos Recursos Naturais (IPR), Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.Department of Biological and Environmental Sciences, University of Jyvaskyla, Jyvaskyla, Finland.Department of Biological and Environmental Sciences, University of Jyvaskyla, Jyvaskyla, Finland.Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.Embrapa Pantanal, Corumbá, Brazil.Universidade Federal de Viçosa, Viçosa, Brazil.Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.Departamento de Virologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.Faculdade de Medicina, Universidade Federal do dos Vales do Jequitinhonha e Mucuri, Diamantina, Brazil.Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.Laboratorio de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. jonatas.abrahao@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29368617

Citation

Andrade, Ana Cláudia Dos S P., et al. "Ubiquitous Giants: a Plethora of Giant Viruses Found in Brazil and Antarctica." Virology Journal, vol. 15, no. 1, 2018, p. 22.
Andrade ACDSP, Arantes TS, Rodrigues RAL, et al. Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica. Virol J. 2018;15(1):22.
Andrade, A. C. D. S. P., Arantes, T. S., Rodrigues, R. A. L., Machado, T. B., Dornas, F. P., Landell, M. F., Furst, C., Borges, L. G. A., Dutra, L. A. L., Almeida, G., Trindade, G. S., Bergier, I., Abrahão, W., Borges, I. A., Cortines, J. R., de Oliveira, D. B., Kroon, E. G., & Abrahão, J. S. (2018). Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica. Virology Journal, 15(1), 22. https://doi.org/10.1186/s12985-018-0930-x
Andrade ACDSP, et al. Ubiquitous Giants: a Plethora of Giant Viruses Found in Brazil and Antarctica. Virol J. 2018 01 24;15(1):22. PubMed PMID: 29368617.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica. AU - Andrade,Ana Cláudia Dos S P, AU - Arantes,Thalita S, AU - Rodrigues,Rodrigo A L, AU - Machado,Talita B, AU - Dornas,Fábio P, AU - Landell,Melissa F, AU - Furst,Cinthia, AU - Borges,Luiz G A, AU - Dutra,Lara A L, AU - Almeida,Gabriel, AU - Trindade,Giliane de S, AU - Bergier,Ivan, AU - Abrahão,Walter, AU - Borges,Iara A, AU - Cortines,Juliana R, AU - de Oliveira,Danilo B, AU - Kroon,Erna G, AU - Abrahão,Jônatas S, Y1 - 2018/01/24/ PY - 2017/08/17/received PY - 2018/01/12/accepted PY - 2018/1/26/entrez PY - 2018/1/26/pubmed PY - 2018/9/19/medline KW - Antarctica KW - Brazil KW - Cedratvirus KW - Giant viruses KW - Marseillevirus KW - Mimivirus KW - Pandoravirus KW - Prospection SP - 22 EP - 22 JF - Virology journal JO - Virol J VL - 15 IS - 1 N2 - BACKGROUND: Since the discovery of giant viruses infecting amoebae in 2003, many dogmas of virology have been revised and the search for these viruses has been intensified. Over the last few years, several new groups of these viruses have been discovered in various types of samples and environments.In this work, we describe the isolation of 68 giant viruses of amoeba obtained from environmental samples from Brazil and Antarctica. METHODS: Isolated viruses were identified by hemacolor staining, PCR assays and electron microscopy (scanning and/or transmission). RESULTS: A total of 64 viruses belonging to the Mimiviridae family were isolated (26 from lineage A, 13 from lineage B, 2 from lineage C and 23 from unidentified lineages) from different types of samples, including marine water from Antarctica, thus being the first mimiviruses isolated in this extreme environment to date. Furthermore, a marseillevirus was isolated from sewage samples along with two pandoraviruses and a cedratvirus (the third to be isolated in the world so far). CONCLUSIONS: Considering the different type of samples, we found a higher number of viral groups in sewage samples. Our results reinforce the importance of prospective studies in different environmental samples, therefore improving our comprehension about the circulation anddiversity of these viruses in nature. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/29368617/Ubiquitous_giants:_a_plethora_of_giant_viruses_found_in_Brazil_and_Antarctica_ L2 - https://virologyj.biomedcentral.com/articles/10.1186/s12985-018-0930-x DB - PRIME DP - Unbound Medicine ER -