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Molecular Characterization and Clonal Diversity of Methicillin-Resistant and -Susceptible Staphylococcus aureus Isolates of Milk of Cows with Clinical Mastitis in Tunisia.
Microb Drug Resist. 2018 Oct; 24(8):1210-1216.MD

Abstract

The aim of this study was to determine the genetic lineages, and the frequency of antibiotic resistance and virulence determinants in methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) isolates recovered from milk of cows with clinical mastitis. Three hundred milk samples from bovine with clinical mastitis were obtained from 30 dairy farms in different regions of Tunisia. Fifteen of the 300 tested samples contained S. aureus (5%), in three cases were MRSA. Isolates (one/sample) were typed (S. aureus protein A [spa], multilocus sequence typing and accessory gene regulator [agr]). The presence of resistance and virulence genes was analyzed by PCR. The three MRSA isolates contained mecA and blaZ genes (one of them also the msr(A) gene), and carried the enterotoxin gene sen; they were typed as t10381-ST4114 or t267-ST4120, and corresponded to agr type-I. Twelve MSSA isolates were recovered and harbored the blaZ (7 strains) or erm(C) genes (1 strain). The MSSA isolates presented seven different spa-types, associated to new sequence types (STs): t426-ST4118, t267-ST4120, t1773-ST4115, t509-ST4119, t529-ST4117, t2844-ST4113, and t2802-ST4112; most isolates (8/12) were typed as t267/ST4120. All S. aureus isolates were scn-negative, except one MSSA of lineage ST4119 that exhibited the immune evasion cluster type D, and harbored the seg, sei, sem, seo, and seu enterotoxin genes. Four MSSA isolates carried the toxic shock syndrome toxin 1 gene (tst). S. aureus (including MRSA) is an important cause of bovine mastitis, showing isolates with high genetic diversity and high content in virulence genes.

Authors+Show Affiliations

1 Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Developement, Pasteur Institute of Tunis , Univérsité de Tunis El Manar, Tunis, Tunisia .1 Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Developement, Pasteur Institute of Tunis , Univérsité de Tunis El Manar, Tunis, Tunisia .2 Area of Biochemistry and Molecular Biology, University of La Rioja , Logroño, Spain .1 Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Developement, Pasteur Institute of Tunis , Univérsité de Tunis El Manar, Tunis, Tunisia .2 Area of Biochemistry and Molecular Biology, University of La Rioja , Logroño, Spain .2 Area of Biochemistry and Molecular Biology, University of La Rioja , Logroño, Spain .1 Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Developement, Pasteur Institute of Tunis , Univérsité de Tunis El Manar, Tunis, Tunisia .

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29373088

Citation

Klibi, Amira, et al. "Molecular Characterization and Clonal Diversity of Methicillin-Resistant and -Susceptible Staphylococcus Aureus Isolates of Milk of Cows With Clinical Mastitis in Tunisia." Microbial Drug Resistance (Larchmont, N.Y.), vol. 24, no. 8, 2018, pp. 1210-1216.
Klibi A, Jouini A, Gómez P, et al. Molecular Characterization and Clonal Diversity of Methicillin-Resistant and -Susceptible Staphylococcus aureus Isolates of Milk of Cows with Clinical Mastitis in Tunisia. Microb Drug Resist. 2018;24(8):1210-1216.
Klibi, A., Jouini, A., Gómez, P., Slimene, K., Ceballos, S., Torres, C., & Maaroufi, A. (2018). Molecular Characterization and Clonal Diversity of Methicillin-Resistant and -Susceptible Staphylococcus aureus Isolates of Milk of Cows with Clinical Mastitis in Tunisia. Microbial Drug Resistance (Larchmont, N.Y.), 24(8), 1210-1216. https://doi.org/10.1089/mdr.2017.0278
Klibi A, et al. Molecular Characterization and Clonal Diversity of Methicillin-Resistant and -Susceptible Staphylococcus Aureus Isolates of Milk of Cows With Clinical Mastitis in Tunisia. Microb Drug Resist. 2018;24(8):1210-1216. PubMed PMID: 29373088.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular Characterization and Clonal Diversity of Methicillin-Resistant and -Susceptible Staphylococcus aureus Isolates of Milk of Cows with Clinical Mastitis in Tunisia. AU - Klibi,Amira, AU - Jouini,Ahlem, AU - Gómez,Paula, AU - Slimene,Khouloud, AU - Ceballos,Sara, AU - Torres,Carmen, AU - Maaroufi,Abderrazek, Y1 - 2018/01/26/ PY - 2018/1/27/pubmed PY - 2018/12/18/medline PY - 2018/1/27/entrez KW - MLST KW - MRSA KW - MSSA KW - bovine mastitis KW - resistance and virulence genes KW - spa-typing SP - 1210 EP - 1216 JF - Microbial drug resistance (Larchmont, N.Y.) JO - Microb Drug Resist VL - 24 IS - 8 N2 - The aim of this study was to determine the genetic lineages, and the frequency of antibiotic resistance and virulence determinants in methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) isolates recovered from milk of cows with clinical mastitis. Three hundred milk samples from bovine with clinical mastitis were obtained from 30 dairy farms in different regions of Tunisia. Fifteen of the 300 tested samples contained S. aureus (5%), in three cases were MRSA. Isolates (one/sample) were typed (S. aureus protein A [spa], multilocus sequence typing and accessory gene regulator [agr]). The presence of resistance and virulence genes was analyzed by PCR. The three MRSA isolates contained mecA and blaZ genes (one of them also the msr(A) gene), and carried the enterotoxin gene sen; they were typed as t10381-ST4114 or t267-ST4120, and corresponded to agr type-I. Twelve MSSA isolates were recovered and harbored the blaZ (7 strains) or erm(C) genes (1 strain). The MSSA isolates presented seven different spa-types, associated to new sequence types (STs): t426-ST4118, t267-ST4120, t1773-ST4115, t509-ST4119, t529-ST4117, t2844-ST4113, and t2802-ST4112; most isolates (8/12) were typed as t267/ST4120. All S. aureus isolates were scn-negative, except one MSSA of lineage ST4119 that exhibited the immune evasion cluster type D, and harbored the seg, sei, sem, seo, and seu enterotoxin genes. Four MSSA isolates carried the toxic shock syndrome toxin 1 gene (tst). S. aureus (including MRSA) is an important cause of bovine mastitis, showing isolates with high genetic diversity and high content in virulence genes. SN - 1931-8448 UR - https://www.unboundmedicine.com/medline/citation/29373088/Molecular_Characterization_and_Clonal_Diversity_of_Methicillin_Resistant_and__Susceptible_Staphylococcus_aureus_Isolates_of_Milk_of_Cows_with_Clinical_Mastitis_in_Tunisia_ L2 - https://www.liebertpub.com/doi/10.1089/mdr.2017.0278?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -