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Single and joint antibacterial activity of aqueous garlic extract and Manuka honey on extended-spectrum beta-lactamase-producing Escherichia coli.

Abstract

Background

Multidrug resistance and recent technological advances have renewed interest in natural product drug discovery from ancient remedies such as Allium sativum (garlic) and honey. This study assessed antibacterial activity of aqueous garlic extract (AGE) and Manuka honey on extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli.

Methods

Thirty clinical isolates of E. coli were collected and screened for ESBL production by double-disc synergy test. Single and joint antibacterial activity of AGE and Manuka honey against ESBL-producing E. coli were determined by agar well dilution and checkerboard methods, respectively.

Results

Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AGE ranged from 125-250 mg/mL and 250-500 mg/mL, respectively. MIC and MBC of Manuka honey ranged from 12.5-25% v/v and 25-50% v/v, respectively. The combination of AGE and Manuka honey exhibited different effects on selected ESBL-producing E. coli; synergism (1/4H+1/16G), additive (1/8H+1/2G, 1/2H+1/16G), indifference (1/16H+MICG, MICH+1/16G, 2MICH+1/32G) and antagonism (4MICH+1/32G).

Conclusions

Manuka honey exhibited greater antibacterial activity against ESBL-producing E. coli than AGE. Antibacterial activity, and the interaction of AGE and Manuka honey against ESBL-producing E. coli are dependent on their concentration. Studies assessing antibacterial activity of potent phytochemicals in AGE and honey would provide insights to mechanisms of interaction for development of new drug leads.

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  • Authors+Show Affiliations

    ,

    Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria, Nigeria.

    Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria, Nigeria.

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    29373742

    Citation

    TY - JOUR T1 - Single and joint antibacterial activity of aqueous garlic extract and Manuka honey on extended-spectrum beta-lactamase-producing Escherichia coli. AU - Idris,Ainau R, AU - Afegbua,Seniyat L, PY - 2017/08/07/received PY - 2017/12/20/accepted PY - 2018/1/27/pubmed PY - 2018/1/27/medline PY - 2018/1/27/entrez KW - Anitbacterial KW - Escherichia coli KW - Extended-spectrum β-lactamase KW - Garlic KW - Manuka honey KW - Resistance SP - 472 EP - 478 JF - Transactions of the Royal Society of Tropical Medicine and Hygiene JO - Trans. R. Soc. Trop. Med. Hyg. VL - 111 IS - 10 N2 - Background: Multidrug resistance and recent technological advances have renewed interest in natural product drug discovery from ancient remedies such as Allium sativum (garlic) and honey. This study assessed antibacterial activity of aqueous garlic extract (AGE) and Manuka honey on extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli. Methods: Thirty clinical isolates of E. coli were collected and screened for ESBL production by double-disc synergy test. Single and joint antibacterial activity of AGE and Manuka honey against ESBL-producing E. coli were determined by agar well dilution and checkerboard methods, respectively. Results: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AGE ranged from 125-250 mg/mL and 250-500 mg/mL, respectively. MIC and MBC of Manuka honey ranged from 12.5-25% v/v and 25-50% v/v, respectively. The combination of AGE and Manuka honey exhibited different effects on selected ESBL-producing E. coli; synergism (1/4H+1/16G), additive (1/8H+1/2G, 1/2H+1/16G), indifference (1/16H+MICG, MICH+1/16G, 2MICH+1/32G) and antagonism (4MICH+1/32G). Conclusions: Manuka honey exhibited greater antibacterial activity against ESBL-producing E. coli than AGE. Antibacterial activity, and the interaction of AGE and Manuka honey against ESBL-producing E. coli are dependent on their concentration. Studies assessing antibacterial activity of potent phytochemicals in AGE and honey would provide insights to mechanisms of interaction for development of new drug leads. SN - 1878-3503 UR - https://www.unboundmedicine.com/medline/citation/29373742/Single_and_joint_antibacterial_activity_of_aqueous_garlic_extract_and_Manuka_honey_on_extended_spectrum_beta_lactamase_producing_Escherichia_coli_ L2 - https://academic.oup.com/trstmh/article-lookup/doi/10.1093/trstmh/trx084 ER -