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High resolution UPLC-MS/MS profiling of polyphenolics in the methanol extract of Syzygium samarangense leaves and its hepatoprotective activity in rats with CCl4-induced hepatic damage.
Food Chem Toxicol. 2018 Mar; 113:145-153.FC

Abstract

Oxidative stress plays a crucial role in the development of several liver diseases. Many natural polyphenols can attenuate oxidative stress and liver injury. In this study, a phytochemical profiling of a methanol extract from leaves of Syzygium samarangense revealed 92 compounds belonging to flavonoids, phenolic acids, condensed tannins, and ellagitannins. The S. samarangense extract exhibited a noticeable antioxidant activity with an EC50 of 5.80 μg/mL measured by DPPH scavenging capacity assay, 2632 Trolox equivalents, 10 mM Fe2+ equivalents/mg of samples by TEAC and FRAP assays, respectively. The total phenolic content was 419 mg gallic acid equivalent GAE/g extract. In a cell-based model (HaCaT cells), the extract completely inhibited ROS production induced by UVA, and prevented GSH-depletion and p38 phosphorylation. In addition, the extract exhibited a substantial antioxidant and hepatoprotective activities in CCl4-treated rats, with an increase in GSH (reduced glutathione) and SOD (superoxide dismutase) activities by 84.75 and 26.27%, respectively, and a decrease of 19.08, 63.05, 52.21, 37.00, 13.26, and 15.15% in MDA, ALT, AST, TB (total bilirubin), TC (total cholesterol), and TG (total glycerides), respectively. These results were confirmed by histopathological analyses. We believe that Syzygium samarangense is a good candidate for further evaluation as an antioxidant and liver protecting drug.

Authors+Show Affiliations

Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, 69120 Heidelberg, Germany. Electronic address: sobeh@uni-heidelberg.de.Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University Abbassia, 11566 Cairo, Egypt.Department of Pharmacology, Faculty of Medicine, King Abdulaziz University, 21589 Jeddah, Saudi Arabia; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, 11566 Cairo, Egypt.Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte Sant'Angelo, Via Cinthia 4, 80126 Naples, Italy.Department of Chemistry, Humboldt-Universität zu Berlin, Berlin, Germany; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt.Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte Sant'Angelo, Via Cinthia 4, 80126 Naples, Italy.Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University Abbassia, 11566 Cairo, Egypt.Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, 69120 Heidelberg, Germany. Electronic address: wink@uni-heidelberg.de.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29374594

Citation

Sobeh, Mansour, et al. "High Resolution UPLC-MS/MS Profiling of Polyphenolics in the Methanol Extract of Syzygium Samarangense Leaves and Its Hepatoprotective Activity in Rats With CCl4-induced Hepatic Damage." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 113, 2018, pp. 145-153.
Sobeh M, Youssef FS, Esmat A, et al. High resolution UPLC-MS/MS profiling of polyphenolics in the methanol extract of Syzygium samarangense leaves and its hepatoprotective activity in rats with CCl4-induced hepatic damage. Food Chem Toxicol. 2018;113:145-153.
Sobeh, M., Youssef, F. S., Esmat, A., Petruk, G., El-Khatib, A. H., Monti, D. M., Ashour, M. L., & Wink, M. (2018). High resolution UPLC-MS/MS profiling of polyphenolics in the methanol extract of Syzygium samarangense leaves and its hepatoprotective activity in rats with CCl4-induced hepatic damage. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 113, 145-153. https://doi.org/10.1016/j.fct.2018.01.031
Sobeh M, et al. High Resolution UPLC-MS/MS Profiling of Polyphenolics in the Methanol Extract of Syzygium Samarangense Leaves and Its Hepatoprotective Activity in Rats With CCl4-induced Hepatic Damage. Food Chem Toxicol. 2018;113:145-153. PubMed PMID: 29374594.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High resolution UPLC-MS/MS profiling of polyphenolics in the methanol extract of Syzygium samarangense leaves and its hepatoprotective activity in rats with CCl4-induced hepatic damage. AU - Sobeh,Mansour, AU - Youssef,Fadia S, AU - Esmat,Ahmed, AU - Petruk,Ganna, AU - El-Khatib,Ahmed H, AU - Monti,Daria Maria, AU - Ashour,Mohamed L, AU - Wink,Michael, Y1 - 2018/01/31/ PY - 2017/11/07/received PY - 2017/12/20/revised PY - 2018/01/20/accepted PY - 2018/1/29/pubmed PY - 2018/8/16/medline PY - 2018/1/29/entrez KW - Antioxidant KW - Carbon tetrachloride toxicity KW - HR-UPLC-MS/MS KW - HaCaT cells KW - Hepatoprotective activity KW - Syzygium samarangense SP - 145 EP - 153 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 113 N2 - Oxidative stress plays a crucial role in the development of several liver diseases. Many natural polyphenols can attenuate oxidative stress and liver injury. In this study, a phytochemical profiling of a methanol extract from leaves of Syzygium samarangense revealed 92 compounds belonging to flavonoids, phenolic acids, condensed tannins, and ellagitannins. The S. samarangense extract exhibited a noticeable antioxidant activity with an EC50 of 5.80 μg/mL measured by DPPH scavenging capacity assay, 2632 Trolox equivalents, 10 mM Fe2+ equivalents/mg of samples by TEAC and FRAP assays, respectively. The total phenolic content was 419 mg gallic acid equivalent GAE/g extract. In a cell-based model (HaCaT cells), the extract completely inhibited ROS production induced by UVA, and prevented GSH-depletion and p38 phosphorylation. In addition, the extract exhibited a substantial antioxidant and hepatoprotective activities in CCl4-treated rats, with an increase in GSH (reduced glutathione) and SOD (superoxide dismutase) activities by 84.75 and 26.27%, respectively, and a decrease of 19.08, 63.05, 52.21, 37.00, 13.26, and 15.15% in MDA, ALT, AST, TB (total bilirubin), TC (total cholesterol), and TG (total glycerides), respectively. These results were confirmed by histopathological analyses. We believe that Syzygium samarangense is a good candidate for further evaluation as an antioxidant and liver protecting drug. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/29374594/High_resolution_UPLC_MS/MS_profiling_of_polyphenolics_in_the_methanol_extract_of_Syzygium_samarangense_leaves_and_its_hepatoprotective_activity_in_rats_with_CCl4_induced_hepatic_damage_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(18)30031-0 DB - PRIME DP - Unbound Medicine ER -