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Prunella vulgaris L. Exerts a Protective Effect Against Extrinsic Aging Through NF-κB, MAPKs, AP-1, and TGF-β/Smad Signaling Pathways in UVB-Aged Normal Human Dermal Fibroblasts.
Rejuvenation Res. 2018 Aug; 21(4):313-322.RR

Abstract

Prunella vulgaris L., a well-known traditional Chinese herbal medicine, has anti-inflammatory and antioxidant activities. In the present study, the underlying molecular mechanisms of the protective effect of P. vulgaris extract (PVE) were investigated in UVB-irradiated normal human dermal fibroblasts (NHDFs). The mRNA expression of matrix metalloproteinases (MMPs), procollagen type I, and cytokines, such as interleukin-6 (IL-6) and tumor necrosis factor (TNF-α), was determined by reverse transcription-polymerase chain reaction. The expression of anti-photoaging-related signaling molecules in the NF-κB, MAPK/AP-1, and TGF/Smad pathways was assessed by western blot. We observed that PVE blocked the upregulated production of radical oxygen species induced in UVB-irradiated NHDFs in a dose-dependent manner. Treatment with PVE also significantly ameliorated the mRNA levels of MMPs, procollagen type I, TNF-α, and IL-6. In addition, the phosphorylation level of c-Jun and c-Fos was decreased through the attenuated expression levels of p-ERK and p-JNK after treatment with PVE. Furthermore, cells treated with PVE showed inhibited Smad 7 and increased Smad 2/3 expression in the TGF-β/Smad signaling pathway. Hence, synthesis of procollagen type I, a precursor of collagen I, was promoted. These findings indicate that treatment with PVE has a potential protective effect against UVB-induced photoaging and photoinflammation.

Authors+Show Affiliations

College of Life Sciences, Kyung Hee University , Yongin-si, Republic of Korea.College of Life Sciences, Kyung Hee University , Yongin-si, Republic of Korea.College of Life Sciences, Kyung Hee University , Yongin-si, Republic of Korea.College of Life Sciences, Kyung Hee University , Yongin-si, Republic of Korea.College of Life Sciences, Kyung Hee University , Yongin-si, Republic of Korea.College of Life Sciences, Kyung Hee University , Yongin-si, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29378470

Citation

Zhang, Mengyang, et al. "Prunella Vulgaris L. Exerts a Protective Effect Against Extrinsic Aging Through NF-κB, MAPKs, AP-1, and TGF-β/Smad Signaling Pathways in UVB-Aged Normal Human Dermal Fibroblasts." Rejuvenation Research, vol. 21, no. 4, 2018, pp. 313-322.
Zhang M, Hwang E, Lin P, et al. Prunella vulgaris L. Exerts a Protective Effect Against Extrinsic Aging Through NF-κB, MAPKs, AP-1, and TGF-β/Smad Signaling Pathways in UVB-Aged Normal Human Dermal Fibroblasts. Rejuvenation Res. 2018;21(4):313-322.
Zhang, M., Hwang, E., Lin, P., Gao, W., Ngo, H. T. T., & Yi, T. H. (2018). Prunella vulgaris L. Exerts a Protective Effect Against Extrinsic Aging Through NF-κB, MAPKs, AP-1, and TGF-β/Smad Signaling Pathways in UVB-Aged Normal Human Dermal Fibroblasts. Rejuvenation Research, 21(4), 313-322. https://doi.org/10.1089/rej.2017.1971
Zhang M, et al. Prunella Vulgaris L. Exerts a Protective Effect Against Extrinsic Aging Through NF-κB, MAPKs, AP-1, and TGF-β/Smad Signaling Pathways in UVB-Aged Normal Human Dermal Fibroblasts. Rejuvenation Res. 2018;21(4):313-322. PubMed PMID: 29378470.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prunella vulgaris L. Exerts a Protective Effect Against Extrinsic Aging Through NF-κB, MAPKs, AP-1, and TGF-β/Smad Signaling Pathways in UVB-Aged Normal Human Dermal Fibroblasts. AU - Zhang,Mengyang, AU - Hwang,Eunson, AU - Lin,Pei, AU - Gao,Wei, AU - Ngo,Hien T T, AU - Yi,Tae-Hoo, Y1 - 2018/03/12/ PY - 2018/1/31/pubmed PY - 2018/11/10/medline PY - 2018/1/31/entrez KW - AP-1 KW - MAPK KW - NF-κB KW - P. vulgaris L. KW - TGF-β/Smad KW - collagen I SP - 313 EP - 322 JF - Rejuvenation research JO - Rejuvenation Res VL - 21 IS - 4 N2 - Prunella vulgaris L., a well-known traditional Chinese herbal medicine, has anti-inflammatory and antioxidant activities. In the present study, the underlying molecular mechanisms of the protective effect of P. vulgaris extract (PVE) were investigated in UVB-irradiated normal human dermal fibroblasts (NHDFs). The mRNA expression of matrix metalloproteinases (MMPs), procollagen type I, and cytokines, such as interleukin-6 (IL-6) and tumor necrosis factor (TNF-α), was determined by reverse transcription-polymerase chain reaction. The expression of anti-photoaging-related signaling molecules in the NF-κB, MAPK/AP-1, and TGF/Smad pathways was assessed by western blot. We observed that PVE blocked the upregulated production of radical oxygen species induced in UVB-irradiated NHDFs in a dose-dependent manner. Treatment with PVE also significantly ameliorated the mRNA levels of MMPs, procollagen type I, TNF-α, and IL-6. In addition, the phosphorylation level of c-Jun and c-Fos was decreased through the attenuated expression levels of p-ERK and p-JNK after treatment with PVE. Furthermore, cells treated with PVE showed inhibited Smad 7 and increased Smad 2/3 expression in the TGF-β/Smad signaling pathway. Hence, synthesis of procollagen type I, a precursor of collagen I, was promoted. These findings indicate that treatment with PVE has a potential protective effect against UVB-induced photoaging and photoinflammation. SN - 1557-8577 UR - https://www.unboundmedicine.com/medline/citation/29378470/Prunella_vulgaris_L__Exerts_a_Protective_Effect_Against_Extrinsic_Aging_Through_NF_κB_MAPKs_AP_1_and_TGF_β/Smad_Signaling_Pathways_in_UVB_Aged_Normal_Human_Dermal_Fibroblasts_ L2 - https://www.liebertpub.com/doi/full/10.1089/rej.2017.1971?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -