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Distribution of sasX, pvl, and qacA/B genes in epidemic methicillin-resistant Staphylococcus aureus strains isolated from East China.
Infect Drug Resist. 2018; 11:55-59.ID

Abstract

Background

Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen. Various virulence and antiseptic-resistant factors increase the pathogenicity of MRSA strains and allow for increased infection rates.

Purpose

The purpose of this study was to investigate the prevalence and distribution of virulence-associated and antiseptic-resistant genes from epidemic MRSA strains isolated from East China.

Methods

A newly designed multiplex PCR assay was used to assess whether the virulence-associated genes sasX and pvl and the chlorhexidine tolerance gene qacA/B were present in 189 clinical isolates of MRSA. Multilocus sequence typing (MLST) and Staphylococcal protein A (spa) typing of these isolates were also performed. The frequency of these genes in isolates with epidemic sequence types (STs) was investigated.

Results

Twenty STs and 36 spa types with five epidemic clones (ST5-t311, ST59-t437, ST5-t002, ST239-t030, and ST239-t037) were identified. The prevalence of sasX, pvl, and qacA/B in all isolates was 5.8%, 10.1%, and 20.1%, respectively. The prevalences of these genes in isolates with ST5, ST59, ST239, and other ST genetic backgrounds were all significantly different (P<0.001). Isolates that had the highest frequency of sasX, pvl, or qacA/B were ST239 (33.3%), ST59 (28.9%), and ST5 (34.1%), respectively. The gene distribution pattern from all of the isolates showed that sasX-pvl-qacA/B+, sasX-pvl+qacA/B-, and sasX+pvl-qacA/B- were closely associated with epidemic clones ST5-t311, ST59-t437, and ST239-t037, respectively.

Conclusion

There are significant differences in the prevalence of virulence-associated and antiseptic-resistant genes in epidemic MRSA strains. Using this information, more effective control and prevention strategies for nosocomial MRSA infections can be developed.

Authors+Show Affiliations

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Department of Laboratory Medicine, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.Clinical Laboratory, Chunan First People's Hospital, Zhejiang Province People's Hospital Chunan Branch, Hangzhou, China.Clinical Laboratory, Yuhang Hospital of Traditional Chinese Medicine, Hangzhou, China.Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Department of Laboratory Medicine, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29386909

Citation

Kong, Haishen, et al. "Distribution of sasX, Pvl, and qacA/B Genes in Epidemic Methicillin-resistant Staphylococcus Aureus Strains Isolated From East China." Infection and Drug Resistance, vol. 11, 2018, pp. 55-59.
Kong H, Fang L, Jiang R, et al. Distribution of sasX, pvl, and qacA/B genes in epidemic methicillin-resistant Staphylococcus aureus strains isolated from East China. Infect Drug Resist. 2018;11:55-59.
Kong, H., Fang, L., Jiang, R., & Tong, J. (2018). Distribution of sasX, pvl, and qacA/B genes in epidemic methicillin-resistant Staphylococcus aureus strains isolated from East China. Infection and Drug Resistance, 11, 55-59. https://doi.org/10.2147/IDR.S153399
Kong H, et al. Distribution of sasX, Pvl, and qacA/B Genes in Epidemic Methicillin-resistant Staphylococcus Aureus Strains Isolated From East China. Infect Drug Resist. 2018;11:55-59. PubMed PMID: 29386909.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of sasX, pvl, and qacA/B genes in epidemic methicillin-resistant Staphylococcus aureus strains isolated from East China. AU - Kong,Haishen, AU - Fang,Lingmei, AU - Jiang,Rujin, AU - Tong,Jixiang, Y1 - 2018/01/09/ PY - 2018/2/2/entrez PY - 2018/2/2/pubmed PY - 2018/2/2/medline KW - MLST KW - MRSA KW - multiplex PCR KW - pvl KW - qacA/B KW - sasX KW - virulence genes SP - 55 EP - 59 JF - Infection and drug resistance JO - Infect Drug Resist VL - 11 N2 - Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen. Various virulence and antiseptic-resistant factors increase the pathogenicity of MRSA strains and allow for increased infection rates. Purpose: The purpose of this study was to investigate the prevalence and distribution of virulence-associated and antiseptic-resistant genes from epidemic MRSA strains isolated from East China. Methods: A newly designed multiplex PCR assay was used to assess whether the virulence-associated genes sasX and pvl and the chlorhexidine tolerance gene qacA/B were present in 189 clinical isolates of MRSA. Multilocus sequence typing (MLST) and Staphylococcal protein A (spa) typing of these isolates were also performed. The frequency of these genes in isolates with epidemic sequence types (STs) was investigated. Results: Twenty STs and 36 spa types with five epidemic clones (ST5-t311, ST59-t437, ST5-t002, ST239-t030, and ST239-t037) were identified. The prevalence of sasX, pvl, and qacA/B in all isolates was 5.8%, 10.1%, and 20.1%, respectively. The prevalences of these genes in isolates with ST5, ST59, ST239, and other ST genetic backgrounds were all significantly different (P<0.001). Isolates that had the highest frequency of sasX, pvl, or qacA/B were ST239 (33.3%), ST59 (28.9%), and ST5 (34.1%), respectively. The gene distribution pattern from all of the isolates showed that sasX-pvl-qacA/B+, sasX-pvl+qacA/B-, and sasX+pvl-qacA/B- were closely associated with epidemic clones ST5-t311, ST59-t437, and ST239-t037, respectively. Conclusion: There are significant differences in the prevalence of virulence-associated and antiseptic-resistant genes in epidemic MRSA strains. Using this information, more effective control and prevention strategies for nosocomial MRSA infections can be developed. SN - 1178-6973 UR - https://www.unboundmedicine.com/medline/citation/29386909/Distribution_of_sasX_pvl_and_qacA/B_genes_in_epidemic_methicillin_resistant_Staphylococcus_aureus_strains_isolated_from_East_China_ L2 - https://dx.doi.org/10.2147/IDR.S153399 DB - PRIME DP - Unbound Medicine ER -
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