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One-pot strategy for on-site enzyme production, biomass hydrolysis, and ethanol production using the whole solid-state fermentation medium of mixed filamentous fungi.
Biotechnol Prog. 2018 05; 34(3):671-680.BP

Abstract

The efficient use of renewable lignocellulosic feedstocks to obtain biofuels and other bioproducts is a key requirement for a sustainable biobased economy. This requires novel and effective strategies to reduce the cost contribution of the cellulolytic enzymatic cocktails needed to convert the carbohydrates into simple sugars, in order to make large-scale commercial processes economically competitive. Here, we propose the use of the whole solid-state fermentation (SSF) medium of mixed filamentous fungi as an integrated one-pot strategy for on-site enzyme production, biomass hydrolysis, and ethanol production. Ten different individual and mixed cultivations of commonly used industrial filamentous fungi (Aspergillus niger, Aspergillus oryzae, Trichoderma harzianum, and Trichoderma reesei) were performed under SSF and the whole media (without the extraction step) were used in the hydrolysis of pretreated sugarcane bagasse. The cocultivation of T. reesei with A. oryzae increased the amount of glucose released by around 50%, compared with individual cultivations. The release of glucose and reducing sugars achieved using the whole SSF medium was around 3-fold higher than obtained with the enzyme extract. The addition of soybean protein (0.5% w/w) during the hydrolysis reaction further significantly improved the saccharification performance by blocking the lignin and avoiding unproductive adsorption of enzymes. The results of the alcoholic fermentation validated the overall integrated process, with a volumetric ethanol productivity of 4.77 g/L.h, representing 83.5% of the theoretical yield. These findings demonstrate the feasibility of the proposed one-pot integrated strategy using the whole SSF medium of mixed filamentous fungi for on-site enzymes production, biomass hydrolysis, and ethanol production. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:671-680, 2018.

Authors+Show Affiliations

Embrapa Instrumentation, Rua XV de Novembro 1452, São Carlos, SP, 13561-260, Brazil. Graduate Program of Chemical Engineering, Federal University of São Carlos, São Carlos, SP, 13565-905, Brazil.Embrapa Instrumentation, Rua XV de Novembro 1452, São Carlos, SP, 13561-260, Brazil.Graduate Program of Chemical Engineering, Federal University of São Carlos, São Carlos, SP, 13565-905, Brazil. Dept. of Chemical Engineering, Federal University of São Carlos, São Carlos, SP, 13565-905, Brazil.Embrapa Instrumentation, Rua XV de Novembro 1452, São Carlos, SP, 13561-260, Brazil. Graduate Program of Chemical Engineering, Federal University of São Carlos, São Carlos, SP, 13565-905, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29388389

Citation

Maehara, Larissa, et al. "One-pot Strategy for On-site Enzyme Production, Biomass Hydrolysis, and Ethanol Production Using the Whole Solid-state Fermentation Medium of Mixed Filamentous Fungi." Biotechnology Progress, vol. 34, no. 3, 2018, pp. 671-680.
Maehara L, Pereira SC, Silva AJ, et al. One-pot strategy for on-site enzyme production, biomass hydrolysis, and ethanol production using the whole solid-state fermentation medium of mixed filamentous fungi. Biotechnol Prog. 2018;34(3):671-680.
Maehara, L., Pereira, S. C., Silva, A. J., & Farinas, C. S. (2018). One-pot strategy for on-site enzyme production, biomass hydrolysis, and ethanol production using the whole solid-state fermentation medium of mixed filamentous fungi. Biotechnology Progress, 34(3), 671-680. https://doi.org/10.1002/btpr.2619
Maehara L, et al. One-pot Strategy for On-site Enzyme Production, Biomass Hydrolysis, and Ethanol Production Using the Whole Solid-state Fermentation Medium of Mixed Filamentous Fungi. Biotechnol Prog. 2018;34(3):671-680. PubMed PMID: 29388389.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - One-pot strategy for on-site enzyme production, biomass hydrolysis, and ethanol production using the whole solid-state fermentation medium of mixed filamentous fungi. AU - Maehara,Larissa, AU - Pereira,Sandra C, AU - Silva,Adilson J, AU - Farinas,Cristiane S, Y1 - 2018/02/12/ PY - 2017/09/29/received PY - 2018/01/29/revised PY - 2018/2/2/pubmed PY - 2019/7/31/medline PY - 2018/2/2/entrez KW - biofuels KW - biorefinery KW - cocultivation KW - enzyme production KW - mixed cultivation KW - solid-state fermentation SP - 671 EP - 680 JF - Biotechnology progress JO - Biotechnol. Prog. VL - 34 IS - 3 N2 - The efficient use of renewable lignocellulosic feedstocks to obtain biofuels and other bioproducts is a key requirement for a sustainable biobased economy. This requires novel and effective strategies to reduce the cost contribution of the cellulolytic enzymatic cocktails needed to convert the carbohydrates into simple sugars, in order to make large-scale commercial processes economically competitive. Here, we propose the use of the whole solid-state fermentation (SSF) medium of mixed filamentous fungi as an integrated one-pot strategy for on-site enzyme production, biomass hydrolysis, and ethanol production. Ten different individual and mixed cultivations of commonly used industrial filamentous fungi (Aspergillus niger, Aspergillus oryzae, Trichoderma harzianum, and Trichoderma reesei) were performed under SSF and the whole media (without the extraction step) were used in the hydrolysis of pretreated sugarcane bagasse. The cocultivation of T. reesei with A. oryzae increased the amount of glucose released by around 50%, compared with individual cultivations. The release of glucose and reducing sugars achieved using the whole SSF medium was around 3-fold higher than obtained with the enzyme extract. The addition of soybean protein (0.5% w/w) during the hydrolysis reaction further significantly improved the saccharification performance by blocking the lignin and avoiding unproductive adsorption of enzymes. The results of the alcoholic fermentation validated the overall integrated process, with a volumetric ethanol productivity of 4.77 g/L.h, representing 83.5% of the theoretical yield. These findings demonstrate the feasibility of the proposed one-pot integrated strategy using the whole SSF medium of mixed filamentous fungi for on-site enzymes production, biomass hydrolysis, and ethanol production. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:671-680, 2018. SN - 1520-6033 UR - https://www.unboundmedicine.com/medline/citation/29388389/One_pot_strategy_for_on_site_enzyme_production_biomass_hydrolysis_and_ethanol_production_using_the_whole_solid_state_fermentation_medium_of_mixed_filamentous_fungi_ L2 - https://doi.org/10.1002/btpr.2619 DB - PRIME DP - Unbound Medicine ER -