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Caralluma fimbriata and metformin protection of rat pancreas from high fat diet induced oxidative stress.
Biotech Histochem. 2018; 93(3):177-187.BH

Abstract

A high fat diet promotes oxidative stress, which contributes to the development of pancreatic fibrosis. We compared the protective effects of a hydroalcoholic extract of Caralluma fimbriata (CFE) to metformin (Met) in the pancreas of Wistar rats fed a high fat diet. The experimental animals were divided into five groups: control (C), treated with CFE (C + CFE), treated with high fat diet (HFD), high fat diet treated with CFE (HFD + CFE), and high fat diet treated with metformin (Met) (HFD + Met). CFE was administered orally to groups C + CFE and HFD + CFE rats for 90 days. Met was given to the HFD + Met group. After 90 days, oxidative stress markers in the pancreas including reduced glutathione (GSH), lipid oxidation (LO), protein oxidation (PO), and activities of antioxidant and polyol pathway enzymes, aldose reductase (AR) and sorbitol dehydrogenase (SDH) were assayed and tissue histology was examined. Establishment of oxidative stress in high fat diet fed rats was verified by elevated LO and PO, decreased GSH, decreased activities of antioxidants and increased activities of polyol pathway enzymes. Oxidative stress was prevented in HFD + CFE and HFD + Met groups. Group C + CFE exhibited improved antioxidant status compared to group C. CFE treatment prevented high fat diet induced acinar cell degeneration, necrosis, edema and hemorrhage. CFE could be used as adjuvant therapy for preventing or managing high fat diet induced pancreatic damage.

Authors+Show Affiliations

a Department of Biochemistry , Sri Krishnadevaraya University , Anantapuramu , India.a Department of Biochemistry , Sri Krishnadevaraya University , Anantapuramu , India.b Green Chem Herbal Extracts and Formulations , Bangalore , India.a Department of Biochemistry , Sri Krishnadevaraya University , Anantapuramu , India.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

29388484

Citation

Sudhakara, G, et al. "Caralluma Fimbriata and Metformin Protection of Rat Pancreas From High Fat Diet Induced Oxidative Stress." Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission, vol. 93, no. 3, 2018, pp. 177-187.
Sudhakara G, Mallaiah P, Rajendran R, et al. Caralluma fimbriata and metformin protection of rat pancreas from high fat diet induced oxidative stress. Biotech Histochem. 2018;93(3):177-187.
Sudhakara, G., Mallaiah, P., Rajendran, R., & Saralakumari, D. (2018). Caralluma fimbriata and metformin protection of rat pancreas from high fat diet induced oxidative stress. Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission, 93(3), 177-187. https://doi.org/10.1080/10520295.2017.1406615
Sudhakara G, et al. Caralluma Fimbriata and Metformin Protection of Rat Pancreas From High Fat Diet Induced Oxidative Stress. Biotech Histochem. 2018;93(3):177-187. PubMed PMID: 29388484.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Caralluma fimbriata and metformin protection of rat pancreas from high fat diet induced oxidative stress. AU - Sudhakara,G, AU - Mallaiah,P, AU - Rajendran,R, AU - Saralakumari,D, Y1 - 2018/02/01/ PY - 2018/2/2/pubmed PY - 2019/2/9/medline PY - 2018/2/2/entrez KW - Caralluma fimbriata KW - high fat diet KW - oxidative stress KW - pancreas KW - polyol pathway enzymes SP - 177 EP - 187 JF - Biotechnic & histochemistry : official publication of the Biological Stain Commission JO - Biotech Histochem VL - 93 IS - 3 N2 - A high fat diet promotes oxidative stress, which contributes to the development of pancreatic fibrosis. We compared the protective effects of a hydroalcoholic extract of Caralluma fimbriata (CFE) to metformin (Met) in the pancreas of Wistar rats fed a high fat diet. The experimental animals were divided into five groups: control (C), treated with CFE (C + CFE), treated with high fat diet (HFD), high fat diet treated with CFE (HFD + CFE), and high fat diet treated with metformin (Met) (HFD + Met). CFE was administered orally to groups C + CFE and HFD + CFE rats for 90 days. Met was given to the HFD + Met group. After 90 days, oxidative stress markers in the pancreas including reduced glutathione (GSH), lipid oxidation (LO), protein oxidation (PO), and activities of antioxidant and polyol pathway enzymes, aldose reductase (AR) and sorbitol dehydrogenase (SDH) were assayed and tissue histology was examined. Establishment of oxidative stress in high fat diet fed rats was verified by elevated LO and PO, decreased GSH, decreased activities of antioxidants and increased activities of polyol pathway enzymes. Oxidative stress was prevented in HFD + CFE and HFD + Met groups. Group C + CFE exhibited improved antioxidant status compared to group C. CFE treatment prevented high fat diet induced acinar cell degeneration, necrosis, edema and hemorrhage. CFE could be used as adjuvant therapy for preventing or managing high fat diet induced pancreatic damage. SN - 1473-7760 UR - https://www.unboundmedicine.com/medline/citation/29388484/Caralluma_fimbriata_and_metformin_protection_of_rat_pancreas_from_high_fat_diet_induced_oxidative_stress_ L2 - https://www.tandfonline.com/doi/full/10.1080/10520295.2017.1406615 DB - PRIME DP - Unbound Medicine ER -