Tags

Type your tag names separated by a space and hit enter

Differential expression of HLA-DR, -DQ, and -DP antigens on malignant B cells.
J Immunol. 1986 Jun 01; 136(11):4311-8.JI

Abstract

HLA class II antigens mediate interactions among cells involved in the immune response and play an important role in the process of self recognition. We made use of conventional alloantisera and six well-characterized monoclonal antibodies (MoAb) to study the HLA class II antigens on CALLA-positive malignant B cell populations and autologous normal B cell lines. Forty additional HLA class II-specific MoAb were also tested for their ability to bind to these cells. By using indirect immunofluorescence and immune precipitation assays, we find that malignant B cells often fail to express one or more of the three known types of HLA class II antigens. Cell lines with the following five phenotypes have been identified: HLA-DR+, -DQ+, -DP+; HLA-DR+, -DQ-, -DP+; HLA-DR-, -DQ+, -DP+; HLA-DR-, -DQ-, -DP+; and HLA-DR-, -DQ-, -DP-. These cell lines have been used to characterize the subregion specificity of MoAb that react with HLA class II antigens. This work confirms the existence of complicated patterns of serologic cross-reactivity between the three different types of HLA class II molecules. It also increases our understanding of the specificity of individual MoAb, thereby facilitating future investigation of the distribution and function of individual antigens. Our studies are consistent with the proposal that altered expression of HLA antigens on tumors might impair recognition of these cells by the immune system of the host, thereby contributing to the proliferation of a malignant clone.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2939141

Citation

Pesando, J M., and L Graf. "Differential Expression of HLA-DR, -DQ, and -DP Antigens On Malignant B Cells." Journal of Immunology (Baltimore, Md. : 1950), vol. 136, no. 11, 1986, pp. 4311-8.
Pesando JM, Graf L. Differential expression of HLA-DR, -DQ, and -DP antigens on malignant B cells. J Immunol. 1986;136(11):4311-8.
Pesando, J. M., & Graf, L. (1986). Differential expression of HLA-DR, -DQ, and -DP antigens on malignant B cells. Journal of Immunology (Baltimore, Md. : 1950), 136(11), 4311-8.
Pesando JM, Graf L. Differential Expression of HLA-DR, -DQ, and -DP Antigens On Malignant B Cells. J Immunol. 1986 Jun 1;136(11):4311-8. PubMed PMID: 2939141.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression of HLA-DR, -DQ, and -DP antigens on malignant B cells. AU - Pesando,J M, AU - Graf,L, PY - 1986/6/1/pubmed PY - 1986/6/1/medline PY - 1986/6/1/entrez SP - 4311 EP - 8 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 136 IS - 11 N2 - HLA class II antigens mediate interactions among cells involved in the immune response and play an important role in the process of self recognition. We made use of conventional alloantisera and six well-characterized monoclonal antibodies (MoAb) to study the HLA class II antigens on CALLA-positive malignant B cell populations and autologous normal B cell lines. Forty additional HLA class II-specific MoAb were also tested for their ability to bind to these cells. By using indirect immunofluorescence and immune precipitation assays, we find that malignant B cells often fail to express one or more of the three known types of HLA class II antigens. Cell lines with the following five phenotypes have been identified: HLA-DR+, -DQ+, -DP+; HLA-DR+, -DQ-, -DP+; HLA-DR-, -DQ+, -DP+; HLA-DR-, -DQ-, -DP+; and HLA-DR-, -DQ-, -DP-. These cell lines have been used to characterize the subregion specificity of MoAb that react with HLA class II antigens. This work confirms the existence of complicated patterns of serologic cross-reactivity between the three different types of HLA class II molecules. It also increases our understanding of the specificity of individual MoAb, thereby facilitating future investigation of the distribution and function of individual antigens. Our studies are consistent with the proposal that altered expression of HLA antigens on tumors might impair recognition of these cells by the immune system of the host, thereby contributing to the proliferation of a malignant clone. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2939141/Differential_expression_of_HLA_DR__DQ_and__DP_antigens_on_malignant_B_cells_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2939141 DB - PRIME DP - Unbound Medicine ER -