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Mapping and validation of a new QTL for adult-plant resistance to powdery mildew in Chinese elite bread wheat line Zhou8425B.
Theor Appl Genet. 2018 May; 131(5):1063-1071.TA

Abstract

KEY MESSAGE

Four QTLs for adult-plant resistance to powdery mildew were mapped in the Zhou8425B/Chinese Spring population, and a new QTL on chromosome 3B was validated in 103 wheat cultivars derived from Zhou8425B. Zhou8425B is an elite wheat (Triticum aestivum L.) line widely used as a parent in Chinese wheat breeding programs. Identification of genes for adult-plant resistance (APR) to powdery mildew in Zhou8425B is of high importance for continued controlling the disease. In the current study, the high-density Illumina iSelect 90K single-nucleotide polymorphism (SNP) array was used to map quantitative trait loci (QTL) for APR to powdery mildew in 244 recombinant inbred lines derived from the cross Zhou8425B/Chinese Spring. Inclusive composite interval mapping identified QTL on chromosomes 1B, 3B, 4B, and 7D, designated as QPm.caas-1BL.1, QPm.caas-3BS, QPm.caas-4BL.2, and QPm.caas-7DS, respectively. Resistance alleles at the QPm.caas-1BL.1, QPm.caas-3BS, and QPm.caas-4BL.2 loci were contributed by Zhou8425B, whereas that at QPm.caas-7DS was from Chinese Spring. QPm.caas-3BS, likely to be a new APR gene for powdery mildew resistance, was detected in all four environments. One SNP marker closely linked to QPm.caas-3BS was transferred into a semi-thermal asymmetric reverse PCR (STARP) marker and tested on 103 commercial wheat cultivars derived from Zhou8425B. Cultivars with the resistance allele at the QPm.caas-3BS locus had averaged maximum disease severity reduced by 5.3%. This STARP marker can be used for marker-assisted selection in improvement of the level of powdery mildew resistance in wheat breeding.

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Authors+Show Affiliations

Jia A
Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.
Ren Y
College of Agronomy, Henan Agricultural University, 63 Agricultural Road, Zhengzhou, 450002, Henan, China.
Gao F
Crop Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, Heilongjiang, China.
Yin G
Zhoukou Academy of Agricultural Sciences, Zhoukou, 466001, Henan, China.
Liu J
Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.
Guo L
Focom Seed Co. Ltd, 11 Chang Chun Road, Zhengzhou, 450001, Henan, China.
Zheng J
Focom Seed Co. Ltd, 11 Chang Chun Road, Zhengzhou, 450001, Henan, China.
He Z
Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing, 100081, China.
Xia X
Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. xiaxianchun@caas.cn.

MeSH

AscomycotaChromosome MappingDisease ResistanceGenes, PlantGenetic MarkersGenotypePhenotypePlant BreedingPlant DiseasesQuantitative Trait LociTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29392374

Citation

Jia, Aolin, et al. "Mapping and Validation of a New QTL for Adult-plant Resistance to Powdery Mildew in Chinese Elite Bread Wheat Line Zhou8425B." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 131, no. 5, 2018, pp. 1063-1071.
Jia A, Ren Y, Gao F, et al. Mapping and validation of a new QTL for adult-plant resistance to powdery mildew in Chinese elite bread wheat line Zhou8425B. Theor Appl Genet. 2018;131(5):1063-1071.
Jia, A., Ren, Y., Gao, F., Yin, G., Liu, J., Guo, L., Zheng, J., He, Z., & Xia, X. (2018). Mapping and validation of a new QTL for adult-plant resistance to powdery mildew in Chinese elite bread wheat line Zhou8425B. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 131(5), 1063-1071. https://doi.org/10.1007/s00122-018-3058-x
Jia A, et al. Mapping and Validation of a New QTL for Adult-plant Resistance to Powdery Mildew in Chinese Elite Bread Wheat Line Zhou8425B. Theor Appl Genet. 2018;131(5):1063-1071. PubMed PMID: 29392374.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mapping and validation of a new QTL for adult-plant resistance to powdery mildew in Chinese elite bread wheat line Zhou8425B. AU - Jia,Aolin, AU - Ren,Yan, AU - Gao,Fengmei, AU - Yin,Guihong, AU - Liu,Jindong, AU - Guo,Lu, AU - Zheng,Jizhou, AU - He,Zhonghu, AU - Xia,Xianchun, Y1 - 2018/02/01/ PY - 2017/09/19/received PY - 2018/01/12/accepted PY - 2018/2/3/pubmed PY - 2018/4/24/medline PY - 2018/2/3/entrez KW - APR KW - Blumeria tritici f. sp. tritici KW - STARP marker KW - Triticum aestivum KW - Wheat 90K SNP array SP - 1063 EP - 1071 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 131 IS - 5 N2 - KEY MESSAGE: Four QTLs for adult-plant resistance to powdery mildew were mapped in the Zhou8425B/Chinese Spring population, and a new QTL on chromosome 3B was validated in 103 wheat cultivars derived from Zhou8425B. Zhou8425B is an elite wheat (Triticum aestivum L.) line widely used as a parent in Chinese wheat breeding programs. Identification of genes for adult-plant resistance (APR) to powdery mildew in Zhou8425B is of high importance for continued controlling the disease. In the current study, the high-density Illumina iSelect 90K single-nucleotide polymorphism (SNP) array was used to map quantitative trait loci (QTL) for APR to powdery mildew in 244 recombinant inbred lines derived from the cross Zhou8425B/Chinese Spring. Inclusive composite interval mapping identified QTL on chromosomes 1B, 3B, 4B, and 7D, designated as QPm.caas-1BL.1, QPm.caas-3BS, QPm.caas-4BL.2, and QPm.caas-7DS, respectively. Resistance alleles at the QPm.caas-1BL.1, QPm.caas-3BS, and QPm.caas-4BL.2 loci were contributed by Zhou8425B, whereas that at QPm.caas-7DS was from Chinese Spring. QPm.caas-3BS, likely to be a new APR gene for powdery mildew resistance, was detected in all four environments. One SNP marker closely linked to QPm.caas-3BS was transferred into a semi-thermal asymmetric reverse PCR (STARP) marker and tested on 103 commercial wheat cultivars derived from Zhou8425B. Cultivars with the resistance allele at the QPm.caas-3BS locus had averaged maximum disease severity reduced by 5.3%. This STARP marker can be used for marker-assisted selection in improvement of the level of powdery mildew resistance in wheat breeding. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/29392374/Mapping_and_validation_of_a_new_QTL_for_adult_plant_resistance_to_powdery_mildew_in_Chinese_elite_bread_wheat_line_Zhou8425B_ L2 - https://dx.doi.org/10.1007/s00122-018-3058-x DB - PRIME DP - Unbound Medicine ER -