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Mapping and validation of a new QTL for adult-plant resistance to powdery mildew in Chinese elite bread wheat line Zhou8425B.
Theor Appl Genet. 2018 May; 131(5):1063-1071.TA

Abstract

KEY MESSAGE

Four QTLs for adult-plant resistance to powdery mildew were mapped in the Zhou8425B/Chinese Spring population, and a new QTL on chromosome 3B was validated in 103 wheat cultivars derived from Zhou8425B. Zhou8425B is an elite wheat (Triticum aestivum L.) line widely used as a parent in Chinese wheat breeding programs. Identification of genes for adult-plant resistance (APR) to powdery mildew in Zhou8425B is of high importance for continued controlling the disease. In the current study, the high-density Illumina iSelect 90K single-nucleotide polymorphism (SNP) array was used to map quantitative trait loci (QTL) for APR to powdery mildew in 244 recombinant inbred lines derived from the cross Zhou8425B/Chinese Spring. Inclusive composite interval mapping identified QTL on chromosomes 1B, 3B, 4B, and 7D, designated as QPm.caas-1BL.1, QPm.caas-3BS, QPm.caas-4BL.2, and QPm.caas-7DS, respectively. Resistance alleles at the QPm.caas-1BL.1, QPm.caas-3BS, and QPm.caas-4BL.2 loci were contributed by Zhou8425B, whereas that at QPm.caas-7DS was from Chinese Spring. QPm.caas-3BS, likely to be a new APR gene for powdery mildew resistance, was detected in all four environments. One SNP marker closely linked to QPm.caas-3BS was transferred into a semi-thermal asymmetric reverse PCR (STARP) marker and tested on 103 commercial wheat cultivars derived from Zhou8425B. Cultivars with the resistance allele at the QPm.caas-3BS locus had averaged maximum disease severity reduced by 5.3%. This STARP marker can be used for marker-assisted selection in improvement of the level of powdery mildew resistance in wheat breeding.

Authors+Show Affiliations

Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.College of Agronomy, Henan Agricultural University, 63 Agricultural Road, Zhengzhou, 450002, Henan, China.Crop Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, Heilongjiang, China.Zhoukou Academy of Agricultural Sciences, Zhoukou, 466001, Henan, China.Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.Focom Seed Co. Ltd, 11 Chang Chun Road, Zhengzhou, 450001, Henan, China.Focom Seed Co. Ltd, 11 Chang Chun Road, Zhengzhou, 450001, Henan, China.Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing, 100081, China.Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. xiaxianchun@caas.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29392374

Citation

Jia, Aolin, et al. "Mapping and Validation of a New QTL for Adult-plant Resistance to Powdery Mildew in Chinese Elite Bread Wheat Line Zhou8425B." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 131, no. 5, 2018, pp. 1063-1071.
Jia A, Ren Y, Gao F, et al. Mapping and validation of a new QTL for adult-plant resistance to powdery mildew in Chinese elite bread wheat line Zhou8425B. Theor Appl Genet. 2018;131(5):1063-1071.
Jia, A., Ren, Y., Gao, F., Yin, G., Liu, J., Guo, L., Zheng, J., He, Z., & Xia, X. (2018). Mapping and validation of a new QTL for adult-plant resistance to powdery mildew in Chinese elite bread wheat line Zhou8425B. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 131(5), 1063-1071. https://doi.org/10.1007/s00122-018-3058-x
Jia A, et al. Mapping and Validation of a New QTL for Adult-plant Resistance to Powdery Mildew in Chinese Elite Bread Wheat Line Zhou8425B. Theor Appl Genet. 2018;131(5):1063-1071. PubMed PMID: 29392374.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mapping and validation of a new QTL for adult-plant resistance to powdery mildew in Chinese elite bread wheat line Zhou8425B. AU - Jia,Aolin, AU - Ren,Yan, AU - Gao,Fengmei, AU - Yin,Guihong, AU - Liu,Jindong, AU - Guo,Lu, AU - Zheng,Jizhou, AU - He,Zhonghu, AU - Xia,Xianchun, Y1 - 2018/02/01/ PY - 2017/09/19/received PY - 2018/01/12/accepted PY - 2018/2/3/pubmed PY - 2018/4/24/medline PY - 2018/2/3/entrez KW - APR KW - Blumeria tritici f. sp. tritici KW - STARP marker KW - Triticum aestivum KW - Wheat 90K SNP array SP - 1063 EP - 1071 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 131 IS - 5 N2 - KEY MESSAGE: Four QTLs for adult-plant resistance to powdery mildew were mapped in the Zhou8425B/Chinese Spring population, and a new QTL on chromosome 3B was validated in 103 wheat cultivars derived from Zhou8425B. Zhou8425B is an elite wheat (Triticum aestivum L.) line widely used as a parent in Chinese wheat breeding programs. Identification of genes for adult-plant resistance (APR) to powdery mildew in Zhou8425B is of high importance for continued controlling the disease. In the current study, the high-density Illumina iSelect 90K single-nucleotide polymorphism (SNP) array was used to map quantitative trait loci (QTL) for APR to powdery mildew in 244 recombinant inbred lines derived from the cross Zhou8425B/Chinese Spring. Inclusive composite interval mapping identified QTL on chromosomes 1B, 3B, 4B, and 7D, designated as QPm.caas-1BL.1, QPm.caas-3BS, QPm.caas-4BL.2, and QPm.caas-7DS, respectively. Resistance alleles at the QPm.caas-1BL.1, QPm.caas-3BS, and QPm.caas-4BL.2 loci were contributed by Zhou8425B, whereas that at QPm.caas-7DS was from Chinese Spring. QPm.caas-3BS, likely to be a new APR gene for powdery mildew resistance, was detected in all four environments. One SNP marker closely linked to QPm.caas-3BS was transferred into a semi-thermal asymmetric reverse PCR (STARP) marker and tested on 103 commercial wheat cultivars derived from Zhou8425B. Cultivars with the resistance allele at the QPm.caas-3BS locus had averaged maximum disease severity reduced by 5.3%. This STARP marker can be used for marker-assisted selection in improvement of the level of powdery mildew resistance in wheat breeding. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/29392374/Mapping_and_validation_of_a_new_QTL_for_adult_plant_resistance_to_powdery_mildew_in_Chinese_elite_bread_wheat_line_Zhou8425B_ L2 - https://dx.doi.org/10.1007/s00122-018-3058-x DB - PRIME DP - Unbound Medicine ER -