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Enterovirus-D68 (EV-D68) in pediatric patients with respiratory infection: The circulation of a new B3 clade in Italy.
J Clin Virol. 2018 Feb - Mar; 99-100:91-96.JC

Abstract

BACKGROUND

In recent years, several outbreaks due to Enterovirus D-68 (EV-D68) have been reported, and it was confirmed that the virus can cause upper and lower respiratory tract diseases and be associated with the development of neurological problems.

OBJECTIVES

The main aim of this research was to study the genetic characteristics of EV-D68 strains that were circulating in Italy identified during an outbreak of an EV-D68 infection that occurred in Italy during the period March-October 2016.

STUDY DESIGN

A retrospective study of the circulation of different types and subtypes of EV-D68 was performed. Nasopharyngeal swabs were collected from March 2016 through October 2016 in children admitted to the Emergency Room with respiratory diseases.

RESULTS

Among 390 children, 22 (59.1% males; mean age 47 months) were found to be infected by EV-D68 and most of them were immunocompetent (72.7%). Pneumonia was diagnosed in 12 (54.5%) children. Phylogenetic analysis of the VP1 region showed that all the strains identified in this study belonged to clade B3. Within B3 subclade, the Italian EV-D68 strains were most closely related to strains detected in Southern China in 2015 as well as to strains detected in US and the Netherlands in 2016.

CONCLUSIONS

These results showed that EV-D68 infections are a common cause of lower respiratory illness in pediatric age. The circulation of one EV-D68 lineage has been proven in Italy and in the European region during 2016. However, further studies are required to investigate whether some strains or lineages may possess a higher affinity for the lower airway or central nervous system.

Links

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Authors+Show Affiliations

Piralla A
Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
Principi N
Pediatric Highly Intensive Care Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milano, Italy.
Ruggiero L
Pediatric Highly Intensive Care Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milano, Italy.
Girello A
Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
Giardina F
Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
De Sando E
Pediatric Clinic, Fondazione IRCCS Policlinico San Matteo, Università degli Studi di Pavia, Pavia, Italy.
Caimmi S
Pediatric Clinic, Fondazione IRCCS Policlinico San Matteo, Università degli Studi di Pavia, Pavia, Italy.
Bianchini S
Pediatric Highly Intensive Care Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milano, Italy.
Marseglia GL
Pediatric Clinic, Fondazione IRCCS Policlinico San Matteo, Università degli Studi di Pavia, Pavia, Italy.
Lunghi G
Virology Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Pavia, Italy.
Baldanti F
Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy; Department of Clinical Surgical, Diagnostic and Pediatric Sciences, Università degli Studi di Pavia, Pavia, Italy.
Esposito S
Pediatric Clinic, Department of Surgical and Biomedical Sciences, Università degli Studi di Perugia, Piazza Menghini 1, 06129, Perugia, Italy. Electronic address: susanna.esposito@unimi.it.

MeSH

AdolescentCapsid ProteinsChildChild, PreschoolEmergency Service, HospitalEnterovirus D, HumanEnterovirus InfectionsFemaleHumansInfantItalyMaleMolecular EpidemiologyMutationNasopharynxPhylogenyReal-Time Polymerase Chain ReactionRespiratory Tract InfectionsRetrospective StudiesSequence Analysis, DNA

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29396353

Citation

Piralla, Antonio, et al. "Enterovirus-D68 (EV-D68) in Pediatric Patients With Respiratory Infection: the Circulation of a New B3 Clade in Italy." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 99-100, 2018, pp. 91-96.
Piralla A, Principi N, Ruggiero L, et al. Enterovirus-D68 (EV-D68) in pediatric patients with respiratory infection: The circulation of a new B3 clade in Italy. J Clin Virol. 2018;99-100:91-96.
Piralla, A., Principi, N., Ruggiero, L., Girello, A., Giardina, F., De Sando, E., Caimmi, S., Bianchini, S., Marseglia, G. L., Lunghi, G., Baldanti, F., & Esposito, S. (2018). Enterovirus-D68 (EV-D68) in pediatric patients with respiratory infection: The circulation of a new B3 clade in Italy. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 99-100, 91-96. https://doi.org/10.1016/j.jcv.2018.01.005
Piralla A, et al. Enterovirus-D68 (EV-D68) in Pediatric Patients With Respiratory Infection: the Circulation of a New B3 Clade in Italy. J Clin Virol. 2018 Feb - Mar;99-100:91-96. PubMed PMID: 29396353.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enterovirus-D68 (EV-D68) in pediatric patients with respiratory infection: The circulation of a new B3 clade in Italy. AU - Piralla,Antonio, AU - Principi,Nicola, AU - Ruggiero,Luca, AU - Girello,Alessia, AU - Giardina,Federica, AU - De Sando,Elisabetta, AU - Caimmi,Silvia, AU - Bianchini,Sonia, AU - Marseglia,Gian Luigi, AU - Lunghi,Giovanna, AU - Baldanti,Fausto, AU - Esposito,Susanna, Y1 - 2018/01/12/ PY - 2017/10/25/received PY - 2018/01/03/revised PY - 2018/01/11/accepted PY - 2018/2/6/pubmed PY - 2019/7/20/medline PY - 2018/2/4/entrez KW - EV-D68 KW - Enterovirus-D68 KW - Molecular epidemiology KW - Pediatric infectious disease KW - Pneumonia KW - Respiratory infection SP - 91 EP - 96 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 99-100 N2 - BACKGROUND: In recent years, several outbreaks due to Enterovirus D-68 (EV-D68) have been reported, and it was confirmed that the virus can cause upper and lower respiratory tract diseases and be associated with the development of neurological problems. OBJECTIVES: The main aim of this research was to study the genetic characteristics of EV-D68 strains that were circulating in Italy identified during an outbreak of an EV-D68 infection that occurred in Italy during the period March-October 2016. STUDY DESIGN: A retrospective study of the circulation of different types and subtypes of EV-D68 was performed. Nasopharyngeal swabs were collected from March 2016 through October 2016 in children admitted to the Emergency Room with respiratory diseases. RESULTS: Among 390 children, 22 (59.1% males; mean age 47 months) were found to be infected by EV-D68 and most of them were immunocompetent (72.7%). Pneumonia was diagnosed in 12 (54.5%) children. Phylogenetic analysis of the VP1 region showed that all the strains identified in this study belonged to clade B3. Within B3 subclade, the Italian EV-D68 strains were most closely related to strains detected in Southern China in 2015 as well as to strains detected in US and the Netherlands in 2016. CONCLUSIONS: These results showed that EV-D68 infections are a common cause of lower respiratory illness in pediatric age. The circulation of one EV-D68 lineage has been proven in Italy and in the European region during 2016. However, further studies are required to investigate whether some strains or lineages may possess a higher affinity for the lower airway or central nervous system. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/29396353/Enterovirus_D68__EV_D68__in_pediatric_patients_with_respiratory_infection:_The_circulation_of_a_new_B3_clade_in_Italy_ DB - PRIME DP - Unbound Medicine ER -