TY - JOUR T1 - Scandoside Exerts Anti-Inflammatory Effect Via Suppressing NF-κB and MAPK Signaling Pathways in LPS-Induced RAW 264.7 Macrophages. AU - He,Jingyu, AU - Li,Jiafeng, AU - Liu,Han, AU - Yang,Zichao, AU - Zhou,Fenghua, AU - Wei,Ting, AU - Dong,Yaqian, AU - Xue,Hongjiao, AU - Tang,Lan, AU - Liu,Menghua, Y1 - 2018/02/03/ PY - 2018/01/07/received PY - 2018/01/29/revised PY - 2018/01/31/accepted PY - 2018/2/7/entrez PY - 2018/2/7/pubmed PY - 2018/8/8/medline KW - anti-inflammation KW - mitogen-activated protein kinase KW - nuclear transcription factor kappa-B alpaha KW - scandoside JF - International journal of molecular sciences JO - Int J Mol Sci VL - 19 IS - 2 N2 - The iridoids of Hedyotis diffusa Willd play an important role in the anti-inflammatory process, but the specific iridoid with anti-inflammatory effect and its mechanism has not be thoroughly studied. An iridoid compound named scandoside (SCA) was isolated from H. diffusa and its anti-inflammatory effect was investigated in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Its anti-inflammatory mechanism was confirmed by in intro experiments and molecular docking analyses. As results, SCA significantly decreased the productions of nitric oxide (NO), prostaglandin E₂ (PGE₂), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and inhibited the levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), TNF-α and IL-6 messenger RNA (mRNA) expression in LPS-induced RAW 264.7 macrophages. SCA treatment suppressed the phosphorylation of inhibitor of nuclear transcription factor kappa-B alpaha (IκB-α), p38, extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK). The docking data suggested that SCA had great binding abilities to COX-2, iNOS and IκB. Taken together, the results indicated that the anti-inflammatory effect of SCA is due to inhibition of pro-inflammatory cytokines and mediators via suppressing the nuclear transcription factor kappa-B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, which provided useful information for its application and development. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/29401674/Scandoside_Exerts_Anti_Inflammatory_Effect_Via_Suppressing_NF_κB_and_MAPK_Signaling_Pathways_in_LPS_Induced_RAW_264_7_Macrophages_ L2 - https://www.mdpi.com/resolver?pii=ijms19020457 DB - PRIME DP - Unbound Medicine ER -