Validation and application by HPLC for simultaneous determination of vitexin-2″-O-glucoside, vitexin-2″-O-rhamnoside, rutin, vitexin, and hyperoside.
J Pharm Anal. 2011 Nov; 1(4):291-296.JP

Abstract

A simple, precise, and rapid high-performance liquid chromatographic method was developed and validated for the simultaneous determination of vitexin-2″-O-glucoside, vitexin-2″-O-rhamnoside, rutin, vitexin, and hyperoside. The HPLC separation was performed using a Shim-pack VP-ODS C18 column (250 mm×4.6 mm i.d., 5 μm) with the isocratic mobile phase consisting of tetrahydrofuran/ acetonitrile/0.05% phosphoric acid solution (20:3:77, v/v/v), and the flow rate was set at 1.0 mL/min. UV detection was carried out at a wavelength of 360 nm and the whole analysis took 25 min. The method was linear in the range of 4.12-206.00 μg/mL for vitexin-2″-O-glucoside, 4.05-202.50 μg/mL for vitexin-2″-O-rhamnoside, 1.64-82.00 μg/mL for rutin, 1.74-87.00 μg/mL for vitexin, and 1.41-70.60 μg/mL for hyperoside with the correlation coefficient for each analyte more than 0.998. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.6 and 2 ng for vitexin-2″-O-glucoside, 0.6 and 2 ng for vitexin-2″-O-rhamnoside, 0.3 and 1 ng for rutin, 1 and 3 ng for vitexin, and 0.5 and 2 ng for hyperoside, respectively. Intra- and inter-day precision and accuracy (RSD) were less than 3%. The developed HPLC method was successfully applied to the analysis of five flavonoids in hawthorn leaves, hawthorn fruits, and the preparations containing hawthorn leaves or fruits.

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Authors+Show Affiliations

Wang CH

Shaanxi Institute for Food and Drug Control, Xi'an 710061, China.

Wang YX

Shaanxi University of Chinese Medicine, Xianyang 712046, China.

Liu HJ

Shaanxi Institute for Food and Drug Control, Xi'an 710061, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29403712

Citation

Wang, Chang-He, et al. "Validation and Application By HPLC for Simultaneous Determination of vitexin-2″-O-glucoside, vitexin-2″-O-rhamnoside, Rutin, Vitexin, and Hyperoside." Journal of Pharmaceutical Analysis, vol. 1, no. 4, 2011, pp. 291-296.

Wang CH, Wang YX, Liu HJ. Validation and application by HPLC for simultaneous determination of vitexin-2″-O-glucoside, vitexin-2″-O-rhamnoside, rutin, vitexin, and hyperoside. J Pharm Anal. 2011;1(4):291-296.

Wang, C. H., Wang, Y. X., & Liu, H. J. (2011). Validation and application by HPLC for simultaneous determination of vitexin-2″-O-glucoside, vitexin-2″-O-rhamnoside, rutin, vitexin, and hyperoside. Journal of Pharmaceutical Analysis, 1(4), 291-296. https://doi.org/10.1016/j.jpha.2011.09.003

Wang CH, Wang YX, Liu HJ. Validation and Application By HPLC for Simultaneous Determination of vitexin-2″-O-glucoside, vitexin-2″-O-rhamnoside, Rutin, Vitexin, and Hyperoside. J Pharm Anal. 2011;1(4):291-296. PubMed PMID: 29403712.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Validation and application by HPLC for simultaneous determination of vitexin-2″-O-glucoside, vitexin-2″-O-rhamnoside, rutin, vitexin, and hyperoside. AU - Wang,Chang-He, AU - Wang,Yu-Xuan, AU - Liu,Hai-Jing, Y1 - 2011/10/24/ PY - 2011/05/24/received PY - 2011/07/10/accepted PY - 2018/2/7/entrez PY - 2011/11/1/pubmed PY - 2011/11/1/medline KW - Flavonoids KW - Hawthorn KW - High-performance liquid chromatography KW - Leaves and fruits KW - Preparations SP - 291 EP - 296 JF - Journal of pharmaceutical analysis JO - J Pharm Anal VL - 1 IS - 4 N2 - A simple, precise, and rapid high-performance liquid chromatographic method was developed and validated for the simultaneous determination of vitexin-2″-O-glucoside, vitexin-2″-O-rhamnoside, rutin, vitexin, and hyperoside. The HPLC separation was performed using a Shim-pack VP-ODS C18 column (250 mm×4.6 mm i.d., 5 μm) with the isocratic mobile phase consisting of tetrahydrofuran/ acetonitrile/0.05% phosphoric acid solution (20:3:77, v/v/v), and the flow rate was set at 1.0 mL/min. UV detection was carried out at a wavelength of 360 nm and the whole analysis took 25 min. The method was linear in the range of 4.12-206.00 μg/mL for vitexin-2″-O-glucoside, 4.05-202.50 μg/mL for vitexin-2″-O-rhamnoside, 1.64-82.00 μg/mL for rutin, 1.74-87.00 μg/mL for vitexin, and 1.41-70.60 μg/mL for hyperoside with the correlation coefficient for each analyte more than 0.998. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.6 and 2 ng for vitexin-2″-O-glucoside, 0.6 and 2 ng for vitexin-2″-O-rhamnoside, 0.3 and 1 ng for rutin, 1 and 3 ng for vitexin, and 0.5 and 2 ng for hyperoside, respectively. Intra- and inter-day precision and accuracy (RSD) were less than 3%. The developed HPLC method was successfully applied to the analysis of five flavonoids in hawthorn leaves, hawthorn fruits, and the preparations containing hawthorn leaves or fruits. SN - 2095-1779 UR - https://www.unboundmedicine.com/medline/citation/29403712/Validation_and_application_by_HPLC_for_simultaneous_determination_of_vitexin_2″_O_glucoside_vitexin_2″_O_rhamnoside_rutin_vitexin_and_hyperoside_ DB - PRIME DP - Unbound Medicine ER -

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Validation and application by HPLC for simultaneous determination of vitexin-2″-O-glucoside, vitexin-2″-O-rhamnoside, rutin, vitexin, and hyperoside.J Pharm Anal. 2011 Nov; 1(4):291-296.JP