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Aetiological study of viruses causing acute encephalitis syndrome in North West India.
Indian J Med Microbiol. 2017 Oct-Dec; 35(4):529-534.IJ

Abstract

CONTEXT

Acute encephalitis syndrome (AES) is a serious public health problem, caused mainly by viruses. However, the profile of viruses causing AES in Rajasthan is not well characterised.

AIMS

The present study was undertaken to identify the viruses causing AES and develop diagnostic algorithm so as to help in improved diagnosis, treatment, prevention and control.

SETTINGS AND DESIGN

The present study is a hospital-based descriptive, observational study. Samples were processed at Grade-1 DHR/ICMR Viral Research and Diagnostic Laboratory at SMS, Jaipur.

SUBJECTS AND METHODS

Cerebrospinal fluid (CSF) samples were processed for IgM antibody detection by enzyme-linked immunosorbent assay (ELISA) for mumps virus (MPV), measles virus (MV), Rubella virus (RV), Japanese encephalitis virus (JEV), West Nile virus (WNV) and Dengue virus using commercial kits. Nucleic acid was extracted from CSF using automated extraction system. Real-time polymerase chain reaction was done using specific primers and probes for Herpes simplex virus (HSV), Varicella-zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV) and enterovirus (EV).

STATISTICAL ANALYSIS USED

Statistical analysis was done using ANOVA.

RESULTS

Among 3088 patients, 702 (22.7%) patients were positive for one or more viruses. HSV (261;8.45%) was the most common followed by EBV (173;5.6%), VZV (97;3.1%), CMV (68;2.2%), EV (32;1.03%), MPV (27;0.9%), DV (28;0.9%), MV (19;0.6%) and RV (6;0.2%).

CONCLUSIONS

AES occurred sporadically in Rajasthan, samples should be tested first for herpes group of viruses followed by EV or/and for arboviruses depending on season or measles, mumps and RVs in children.

Authors+Show Affiliations

Department of Microbiology and Immunology, Advanced Basic Sciences and Clinical Research Laboratory, ICMR Grade 1/ DHR State, Sawai Man Singh Medical College, Jaipur, Rajasthan, India.Department of Microbiology and Immunology, Advanced Basic Sciences and Clinical Research Laboratory, ICMR Grade 1/ DHR State, Sawai Man Singh Medical College, Jaipur, Rajasthan, India.Department of Microbiology and Immunology, Advanced Basic Sciences and Clinical Research Laboratory, ICMR Grade 1/ DHR State, Sawai Man Singh Medical College, Jaipur, Rajasthan, India.Department of Medicine, Sawai Man Singh Hospital, Jaipur, Rajasthan, India.Department of Microbiology and Immunology, Advanced Basic Sciences and Clinical Research Laboratory, ICMR Grade 1/ DHR State, Sawai Man Singh Medical College, Jaipur, Rajasthan, India.Department of Microbiology and Immunology, Advanced Basic Sciences and Clinical Research Laboratory, ICMR Grade 1/ DHR State, Sawai Man Singh Medical College, Jaipur, Rajasthan, India.Department of Microbiology and Immunology, Advanced Basic Sciences and Clinical Research Laboratory, ICMR Grade 1/ DHR State, Sawai Man Singh Medical College, Jaipur, Rajasthan, India.Department of Microbiology and Immunology, Advanced Basic Sciences and Clinical Research Laboratory, ICMR Grade 1/ DHR State, Sawai Man Singh Medical College, Jaipur, Rajasthan, India.

Pub Type(s)

Journal Article
Observational Study

Language

eng

PubMed ID

29405145

Citation

Tiwari, Jitendra Kumar, et al. "Aetiological Study of Viruses Causing Acute Encephalitis Syndrome in North West India." Indian Journal of Medical Microbiology, vol. 35, no. 4, 2017, pp. 529-534.
Tiwari JK, Malhotra B, Chauhan A, et al. Aetiological study of viruses causing acute encephalitis syndrome in North West India. Indian J Med Microbiol. 2017;35(4):529-534.
Tiwari, J. K., Malhotra, B., Chauhan, A., Malhotra, H., Sharma, P., Deeba, F., Trivedi, K., & Swamy, A. M. (2017). Aetiological study of viruses causing acute encephalitis syndrome in North West India. Indian Journal of Medical Microbiology, 35(4), 529-534. https://doi.org/10.4103/ijmm.IJMM_17_180
Tiwari JK, et al. Aetiological Study of Viruses Causing Acute Encephalitis Syndrome in North West India. Indian J Med Microbiol. 2017 Oct-Dec;35(4):529-534. PubMed PMID: 29405145.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aetiological study of viruses causing acute encephalitis syndrome in North West India. AU - Tiwari,Jitendra Kumar, AU - Malhotra,Bharti, AU - Chauhan,Aradhana, AU - Malhotra,Hemant, AU - Sharma,Pratibha, AU - Deeba,Farah, AU - Trivedi,Khushbu, AU - Swamy,Anjenya M, PY - 2018/2/7/entrez PY - 2018/2/7/pubmed PY - 2018/8/25/medline SP - 529 EP - 534 JF - Indian journal of medical microbiology JO - Indian J Med Microbiol VL - 35 IS - 4 N2 - CONTEXT: Acute encephalitis syndrome (AES) is a serious public health problem, caused mainly by viruses. However, the profile of viruses causing AES in Rajasthan is not well characterised. AIMS: The present study was undertaken to identify the viruses causing AES and develop diagnostic algorithm so as to help in improved diagnosis, treatment, prevention and control. SETTINGS AND DESIGN: The present study is a hospital-based descriptive, observational study. Samples were processed at Grade-1 DHR/ICMR Viral Research and Diagnostic Laboratory at SMS, Jaipur. SUBJECTS AND METHODS: Cerebrospinal fluid (CSF) samples were processed for IgM antibody detection by enzyme-linked immunosorbent assay (ELISA) for mumps virus (MPV), measles virus (MV), Rubella virus (RV), Japanese encephalitis virus (JEV), West Nile virus (WNV) and Dengue virus using commercial kits. Nucleic acid was extracted from CSF using automated extraction system. Real-time polymerase chain reaction was done using specific primers and probes for Herpes simplex virus (HSV), Varicella-zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV) and enterovirus (EV). STATISTICAL ANALYSIS USED: Statistical analysis was done using ANOVA. RESULTS: Among 3088 patients, 702 (22.7%) patients were positive for one or more viruses. HSV (261;8.45%) was the most common followed by EBV (173;5.6%), VZV (97;3.1%), CMV (68;2.2%), EV (32;1.03%), MPV (27;0.9%), DV (28;0.9%), MV (19;0.6%) and RV (6;0.2%). CONCLUSIONS: AES occurred sporadically in Rajasthan, samples should be tested first for herpes group of viruses followed by EV or/and for arboviruses depending on season or measles, mumps and RVs in children. SN - 1998-3646 UR - https://www.unboundmedicine.com/medline/citation/29405145/Aetiological_study_of_viruses_causing_acute_encephalitis_syndrome_in_North_West_India_ L2 - https://linkinghub.elsevier.com/retrieve/pii/IndianJMedMicrobiol_2017_35_4_529_224424 DB - PRIME DP - Unbound Medicine ER -