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Citrate-capped silver nanoparticles as a probe for sensitive and selective colorimetric and spectrophotometric sensing of creatinine in human urine.
Anal Chim Acta. 2018 May 12; 1007:40-49.AC

Abstract

Urinary creatinine concentration is a critical physiological parameter that enables reliable assessment of patient renal function and diagnosis of a broad spectrum of diseases. In this study, a simple and inexpensive sensor comprising monodisperse, citrate-capped silver nanoparticles (cc-AgNPs) was developed, which enabled rapid, sensitive and selective quantitation of creatinine directly in unprocessed urine. The mechanism of this sensor entails the creatinine-mediated aggregation of the cc-AgNPs (within 1 min) under alkaline conditions (pH 12). This is attributed to the tautomerization of creatinine to its amino anionic species at alkaline pH, which cross-link the cc-AgNPs via hydrogen bond networks with the negatively charged citrate caps. Creatinine elicited visibly-discernable color changes of the cc-AgNPs colloids in a concentration-dependent manner up to 10 μM. UV-visible spectroscopic analyses of the cc-AgNPs revealed that creatinine elicited a concentration-dependent decrease in intensity of the localized surface plasmon resonance (LSPR) band centered around 403 nm, with a concomitant increase in intensity of the red-shifted LSPR band at 670 nm. This observation denotes a creatinine-mediated increase in cc-AgNP particle size via aggregation, as confirmed by transmission electron microscopy analysis. The cc-AgNP sensor exhibited a linear correlation between the A670/A403 extinction ratio and creatinine concentration range of 0-4.2 μM in aqueous solutions (R2 = 0.996), and a low detection limit of 53.4 nM. Hence, the simplicity, short assay time, and high sensitivity and selectivity of our cc-AgNP sensor affirms its utility as a creatinine monitoring assay for low-resource, point-of-care settings.

Authors+Show Affiliations

College of Sciences, Department of Chemical and Forensic Sciences, Botswana International University of Science and Technology, Plot 10071, Private Bag 16, Palapye, Botswana.Institute of Infectious Disease and Molecular Medicine, Department of Integrative Biomedical Sciences, University of Cape Town, Cape Town 7925, South Africa.Institute of Infectious Disease and Molecular Medicine, Department of Integrative Biomedical Sciences, University of Cape Town, Cape Town 7925, South Africa.Institute of Infectious Disease and Molecular Medicine, Department of Integrative Biomedical Sciences, University of Cape Town, Cape Town 7925, South Africa. Electronic address: jonathan.blackburn@uct.ac.za.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29405987

Citation

Alula, Melisew Tadele, et al. "Citrate-capped Silver Nanoparticles as a Probe for Sensitive and Selective Colorimetric and Spectrophotometric Sensing of Creatinine in Human Urine." Analytica Chimica Acta, vol. 1007, 2018, pp. 40-49.
Alula MT, Karamchand L, Hendricks NR, et al. Citrate-capped silver nanoparticles as a probe for sensitive and selective colorimetric and spectrophotometric sensing of creatinine in human urine. Anal Chim Acta. 2018;1007:40-49.
Alula, M. T., Karamchand, L., Hendricks, N. R., & Blackburn, J. M. (2018). Citrate-capped silver nanoparticles as a probe for sensitive and selective colorimetric and spectrophotometric sensing of creatinine in human urine. Analytica Chimica Acta, 1007, 40-49. https://doi.org/10.1016/j.aca.2017.12.016
Alula MT, et al. Citrate-capped Silver Nanoparticles as a Probe for Sensitive and Selective Colorimetric and Spectrophotometric Sensing of Creatinine in Human Urine. Anal Chim Acta. 2018 May 12;1007:40-49. PubMed PMID: 29405987.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Citrate-capped silver nanoparticles as a probe for sensitive and selective colorimetric and spectrophotometric sensing of creatinine in human urine. AU - Alula,Melisew Tadele, AU - Karamchand,Leshern, AU - Hendricks,Nicolette R, AU - Blackburn,Jonathan M, Y1 - 2017/12/28/ PY - 2017/10/30/received PY - 2017/12/06/accepted PY - 2018/2/7/entrez PY - 2018/2/7/pubmed PY - 2018/8/14/medline KW - Aggregation KW - Citrate-capped silver nanoparticles KW - Creatinine KW - Localized surface plasmon resonance (LSPR) KW - UV-Visible spectrophotometry SP - 40 EP - 49 JF - Analytica chimica acta JO - Anal Chim Acta VL - 1007 N2 - Urinary creatinine concentration is a critical physiological parameter that enables reliable assessment of patient renal function and diagnosis of a broad spectrum of diseases. In this study, a simple and inexpensive sensor comprising monodisperse, citrate-capped silver nanoparticles (cc-AgNPs) was developed, which enabled rapid, sensitive and selective quantitation of creatinine directly in unprocessed urine. The mechanism of this sensor entails the creatinine-mediated aggregation of the cc-AgNPs (within 1 min) under alkaline conditions (pH 12). This is attributed to the tautomerization of creatinine to its amino anionic species at alkaline pH, which cross-link the cc-AgNPs via hydrogen bond networks with the negatively charged citrate caps. Creatinine elicited visibly-discernable color changes of the cc-AgNPs colloids in a concentration-dependent manner up to 10 μM. UV-visible spectroscopic analyses of the cc-AgNPs revealed that creatinine elicited a concentration-dependent decrease in intensity of the localized surface plasmon resonance (LSPR) band centered around 403 nm, with a concomitant increase in intensity of the red-shifted LSPR band at 670 nm. This observation denotes a creatinine-mediated increase in cc-AgNP particle size via aggregation, as confirmed by transmission electron microscopy analysis. The cc-AgNP sensor exhibited a linear correlation between the A670/A403 extinction ratio and creatinine concentration range of 0-4.2 μM in aqueous solutions (R2 = 0.996), and a low detection limit of 53.4 nM. Hence, the simplicity, short assay time, and high sensitivity and selectivity of our cc-AgNP sensor affirms its utility as a creatinine monitoring assay for low-resource, point-of-care settings. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/29405987/Citrate_capped_silver_nanoparticles_as_a_probe_for_sensitive_and_selective_colorimetric_and_spectrophotometric_sensing_of_creatinine_in_human_urine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(17)31427-7 DB - PRIME DP - Unbound Medicine ER -