Citation
Maciel, Laércio Galvão, et al. "Hibiscus Sabdariffa Anthocyanins-rich Extract: Chemical Stability, in Vitro Antioxidant and Antiproliferative Activities." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 113, 2018, pp. 187-197.
Maciel LG, do Carmo MAV, Azevedo L, et al. Hibiscus sabdariffa anthocyanins-rich extract: Chemical stability, in vitro antioxidant and antiproliferative activities. Food Chem Toxicol. 2018;113:187-197.
Maciel, L. G., do Carmo, M. A. V., Azevedo, L., Daguer, H., Molognoni, L., de Almeida, M. M., Granato, D., & Rosso, N. D. (2018). Hibiscus sabdariffa anthocyanins-rich extract: Chemical stability, in vitro antioxidant and antiproliferative activities. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 113, 187-197. https://doi.org/10.1016/j.fct.2018.01.053
Maciel LG, et al. Hibiscus Sabdariffa Anthocyanins-rich Extract: Chemical Stability, in Vitro Antioxidant and Antiproliferative Activities. Food Chem Toxicol. 2018;113:187-197. PubMed PMID: 29407472.
TY - JOUR
T1 - Hibiscus sabdariffa anthocyanins-rich extract: Chemical stability, in vitro antioxidant and antiproliferative activities.
AU - Maciel,Laércio Galvão,
AU - do Carmo,Mariana Araújo Vieira,
AU - Azevedo,Luciana,
AU - Daguer,Heitor,
AU - Molognoni,Luciano,
AU - de Almeida,Mereci Mendes,
AU - Granato,Daniel,
AU - Rosso,Neiva Deliberali,
Y1 - 2018/01/31/
PY - 2017/11/16/received
PY - 2018/01/19/revised
PY - 2018/01/29/accepted
PY - 2018/2/7/pubmed
PY - 2018/8/16/medline
PY - 2018/2/7/entrez
KW - Anthocyanins
KW - Copigmentation
KW - Cytotoxicity
KW - Functional foods
KW - LC-ESI-MS
KW - Response surface methodology
SP - 187
EP - 197
JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
JO - Food Chem Toxicol
VL - 113
N2 - Hibiscus sabdariffa calyx is a rich source of anthocyanins and other bioactive compounds but no study reported the effects of experimental conditions on the extraction of these chemical compounds. Therefore, the effects of time and extraction temperature on the bioactive compounds and antioxidant activity of Hibiscus sabdariffa calyx were evaluated. In addition, the effects of copigmentation and pH on the stability of anthocyanins were assessed and the cytotoxic effects (LC50, IC50, and GC50) of the extracts were determined in relation to tumor cell lines - Caco-2, HepG-2, HCT8, and A549. The temperature significantly influenced the total anthocyanins and flavonoids contents. The interaction between time/temperature influenced the total phenolic content and ascorbic acid. The t1/2 and the percentage of colour retention decreased markedly at temperatures above 80 °C. Variations in pH conserved the antioxidant activity of the anthocyanins, and the protonation-deprotonation process of the extract was reversible. The treatment of cells with purified anthocyanin extract or crude extracts at 5-800 μg mL-1 did not show significant cytotoxic effects on the cell lines, corroborating the chemical antioxidant effect of the extracts (DPPH assay). Cyanidin-3-glucoside, delphinidin-3-sambubioside, delphinidin-3-glucoside, and cyanidin-3-sambubioside were identified in the extracts by LC-ESI-MS.
SN - 1873-6351
UR - https://www.unboundmedicine.com/medline/citation/29407472/Hibiscus_sabdariffa_anthocyanins_rich_extract:_Chemical_stability_in_vitro_antioxidant_and_antiproliferative_activities_
DB - PRIME
DP - Unbound Medicine
ER -
