Tags

Type your tag names separated by a space and hit enter

Prevalence, genetic relatedness and antibiotic resistance of hospital-acquired clostridium difficile PCR ribotype 018 strains.
Int J Antimicrob Agents. 2018 May; 51(5):762-767.IJ

Abstract

Clostridium difficile infection (CDI) is a major healthcare-associated infection. The aim of this study was to investigate the genetic relatedness of the endemic C. difficile PCR ribotype 018 strains in an institution and changes to their characteristics during a five-year period. A total of 207 isolates from inpatients at Hanyang University Hospital from 2009 to 2013 were analysed using multilocus variable-number tandem-repeat analysis (MLVA). Minimum inhibitory concentrations (MICs) of several antibiotics were determined. In total, 204 (98.6%) were genetically related, with a summed tandem-repeat distance (STRD) ≤ 10. Minimum-spanning-tree analysis identified 78 MLVA types, categorized into six clonal complexes (CCs). The largest cluster, CC-I, included 51 MLVA types from 148 isolates (71.5%) and the second largest cluster, CC-II, included 10 MLVA types from 36 isolates (17.4%). Resistance rates for antibiotics were: clindamycin (CLI), 97.6%; moxifloxacin (MXF), 98.6%; vancomycin (VAN), 1.4%; and rifaximin (RFX), 8.2%. All isolates were susceptible to piperacillin/tazobactam (TZP) and metronidazole (MTZ). Comparing the MICs of antibiotics for the isolates each year from 2009 to 2013, MICs of antibiotics that promote CDI, such as CLI, MXF, TZP and RFX, increased over the five-year period (P-value by Kruskal-Wallis test: < 0.0001, <0.0001, <0.0001, and <0.0001 respectively); however, MICs of VAN or MTZ, antibiotics for treatment of CDI, did not increase or decreased over the same time period (P-value by Kruskal-Wallis test: 0.166, <0.0001). C. difficile RT018 isolates in a tertiary hospital over a five-year period presented a close clonal relationship. MICs of antibiotics promoting CDI increased with this clonal expansion.

Authors+Show Affiliations

Department of Internal Medicine, College of Medicine, Hanyang University, Seoul, Republic of Korea.Department of Internal Medicine, College of Medicine, Hanyang University, Seoul, Republic of Korea.Laboratory Medicine, College of Medicine, Hanyang University, Seoul, Republic of Korea.Center for Chronic Diseases, Research Institute, National Medical Center, Seoul, Republic of Korea.Department of Internal Medicine, College of Medicine, Hanyang University, Seoul, Republic of Korea. Electronic address: paihj@hanyang.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29408226

Citation

Seo, Mi-Ran, et al. "Prevalence, Genetic Relatedness and Antibiotic Resistance of Hospital-acquired Clostridium Difficile PCR Ribotype 018 Strains." International Journal of Antimicrobial Agents, vol. 51, no. 5, 2018, pp. 762-767.
Seo MR, Kim J, Lee Y, et al. Prevalence, genetic relatedness and antibiotic resistance of hospital-acquired clostridium difficile PCR ribotype 018 strains. Int J Antimicrob Agents. 2018;51(5):762-767.
Seo, M. R., Kim, J., Lee, Y., Lim, D. G., & Pai, H. (2018). Prevalence, genetic relatedness and antibiotic resistance of hospital-acquired clostridium difficile PCR ribotype 018 strains. International Journal of Antimicrobial Agents, 51(5), 762-767. https://doi.org/10.1016/j.ijantimicag.2018.01.025
Seo MR, et al. Prevalence, Genetic Relatedness and Antibiotic Resistance of Hospital-acquired Clostridium Difficile PCR Ribotype 018 Strains. Int J Antimicrob Agents. 2018;51(5):762-767. PubMed PMID: 29408226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence, genetic relatedness and antibiotic resistance of hospital-acquired clostridium difficile PCR ribotype 018 strains. AU - Seo,Mi-Ran, AU - Kim,Jieun, AU - Lee,Yangsoon, AU - Lim,Dong-Gyun, AU - Pai,Hyunjoo, Y1 - 2018/02/02/ PY - 2017/10/26/received PY - 2018/01/22/revised PY - 2018/01/27/accepted PY - 2018/2/7/pubmed PY - 2018/9/25/medline PY - 2018/2/7/entrez KW - Antimicrobials KW - Clostridium difficile KW - MLVA KW - Minimum inhibitory concentration KW - PCR ribotype 018 SP - 762 EP - 767 JF - International journal of antimicrobial agents JO - Int J Antimicrob Agents VL - 51 IS - 5 N2 - Clostridium difficile infection (CDI) is a major healthcare-associated infection. The aim of this study was to investigate the genetic relatedness of the endemic C. difficile PCR ribotype 018 strains in an institution and changes to their characteristics during a five-year period. A total of 207 isolates from inpatients at Hanyang University Hospital from 2009 to 2013 were analysed using multilocus variable-number tandem-repeat analysis (MLVA). Minimum inhibitory concentrations (MICs) of several antibiotics were determined. In total, 204 (98.6%) were genetically related, with a summed tandem-repeat distance (STRD) ≤ 10. Minimum-spanning-tree analysis identified 78 MLVA types, categorized into six clonal complexes (CCs). The largest cluster, CC-I, included 51 MLVA types from 148 isolates (71.5%) and the second largest cluster, CC-II, included 10 MLVA types from 36 isolates (17.4%). Resistance rates for antibiotics were: clindamycin (CLI), 97.6%; moxifloxacin (MXF), 98.6%; vancomycin (VAN), 1.4%; and rifaximin (RFX), 8.2%. All isolates were susceptible to piperacillin/tazobactam (TZP) and metronidazole (MTZ). Comparing the MICs of antibiotics for the isolates each year from 2009 to 2013, MICs of antibiotics that promote CDI, such as CLI, MXF, TZP and RFX, increased over the five-year period (P-value by Kruskal-Wallis test: < 0.0001, <0.0001, <0.0001, and <0.0001 respectively); however, MICs of VAN or MTZ, antibiotics for treatment of CDI, did not increase or decreased over the same time period (P-value by Kruskal-Wallis test: 0.166, <0.0001). C. difficile RT018 isolates in a tertiary hospital over a five-year period presented a close clonal relationship. MICs of antibiotics promoting CDI increased with this clonal expansion. SN - 1872-7913 UR - https://www.unboundmedicine.com/medline/citation/29408226/Prevalence_genetic_relatedness_and_antibiotic_resistance_of_hospital_acquired_clostridium_difficile_PCR_ribotype_018_strains_ DB - PRIME DP - Unbound Medicine ER -