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Mid-gestational sevoflurane exposure inhibits fetal neural stem cell proliferation and impairs postnatal learning and memory function in a dose-dependent manner.
Dev Biol. 2018 03 15; 435(2):185-197.DB

Abstract

Advancements in fetal intervention procedures have led to increases in the number of pregnant women undergoing general anesthesia during the second trimester-a period characterized by extensive proliferation of fetal neural stem cells (NSCs). However, few studies have investigated the effects of mid-gestational sevoflurane exposure on fetal NSC proliferation or postnatal learning and memory function. In the present study, pregnant rats were randomly assigned to a control group (C group), a low sevoflurane concentration group (2%; L group), a high sevoflurane concentration group (3.5%; H group), a high sevoflurane concentration plus lithium chloride group (H + Li group), and a lithium chloride group (Li group) at gestational day 14. Rats received different concentrations of sevoflurane anesthesia for 2 h. The offspring rats were weaned at 28 days for behavioral testing (i.e., Morris Water Maze [MWM]), and fetal brains or postnatal hippocampal tissues were harvested for immunofluorescence staining, real-time PCR, and Western blotting analyses in order to determine the effect of sevoflurane exposure on NSC proliferation and the Wnt/β-catenin signaling pathway. Our results indicated that maternal exposure to 3.5% sevoflurane (H group) during the mid-gestational period impaired the performance of offspring rats in the MWM test, reduced NSC proliferation, and increased protein levels of fetal glycogen synthase kinase-3 beta (GSK-3β). Such treatment also decreased levels of β-catenin protein, CD44 RNA, and Cyclin D1 RNA relative to those observed in the C group. However, these effects were transiently attenuated by treatment with lithium chloride. Conversely, maternal exposure to 2% sevoflurane (L group) did not influence NSC proliferation or the Wnt signaling pathway. Our results suggest that sevoflurane exposure during the second trimester inhibits fetal NSC proliferation via the Wnt/β-catenin pathway and impairs postnatal learning and memory function in a dose-dependent manner.

Authors+Show Affiliations

Department of Anesthesiology, Shengjing Hospital of China Medical University, China Medical University, Shenyang 110004, China.Department of Obstetrics, Shengjing Hospital of China Medical University, China Medical University, Shenyang 110004, China.Department of Anesthesiology, Shengjing Hospital of China Medical University, China Medical University, Shenyang 110004, China.Department of Anesthesiology, Shengjing Hospital of China Medical University, China Medical University, Shenyang 110004, China.Department of Neuroendocrine Pharmacology, School of Pharmacy, China Medical University, Shenyang 110122, China.Department of Anesthesiology, Shengjing Hospital of China Medical University, China Medical University, Shenyang 110004, China. Electronic address: zhaop@sj-hospital.org.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29410165

Citation

Wang, Yuan, et al. "Mid-gestational Sevoflurane Exposure Inhibits Fetal Neural Stem Cell Proliferation and Impairs Postnatal Learning and Memory Function in a Dose-dependent Manner." Developmental Biology, vol. 435, no. 2, 2018, pp. 185-197.
Wang Y, Yin S, Xue H, et al. Mid-gestational sevoflurane exposure inhibits fetal neural stem cell proliferation and impairs postnatal learning and memory function in a dose-dependent manner. Dev Biol. 2018;435(2):185-197.
Wang, Y., Yin, S., Xue, H., Yang, Y., Zhang, N., & Zhao, P. (2018). Mid-gestational sevoflurane exposure inhibits fetal neural stem cell proliferation and impairs postnatal learning and memory function in a dose-dependent manner. Developmental Biology, 435(2), 185-197. https://doi.org/10.1016/j.ydbio.2018.01.022
Wang Y, et al. Mid-gestational Sevoflurane Exposure Inhibits Fetal Neural Stem Cell Proliferation and Impairs Postnatal Learning and Memory Function in a Dose-dependent Manner. Dev Biol. 2018 03 15;435(2):185-197. PubMed PMID: 29410165.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mid-gestational sevoflurane exposure inhibits fetal neural stem cell proliferation and impairs postnatal learning and memory function in a dose-dependent manner. AU - Wang,Yuan, AU - Yin,Shaowei, AU - Xue,Hang, AU - Yang,Yating, AU - Zhang,Nan, AU - Zhao,Ping, Y1 - 2018/02/02/ PY - 2017/08/19/received PY - 2018/01/23/revised PY - 2018/01/30/accepted PY - 2018/2/8/pubmed PY - 2018/4/6/medline PY - 2018/2/8/entrez KW - Mid-gestation KW - Neural stem cells KW - Pregnancy KW - Proliferation KW - Sevoflurane KW - Wnt signaling pathway SP - 185 EP - 197 JF - Developmental biology JO - Dev. Biol. VL - 435 IS - 2 N2 - Advancements in fetal intervention procedures have led to increases in the number of pregnant women undergoing general anesthesia during the second trimester-a period characterized by extensive proliferation of fetal neural stem cells (NSCs). However, few studies have investigated the effects of mid-gestational sevoflurane exposure on fetal NSC proliferation or postnatal learning and memory function. In the present study, pregnant rats were randomly assigned to a control group (C group), a low sevoflurane concentration group (2%; L group), a high sevoflurane concentration group (3.5%; H group), a high sevoflurane concentration plus lithium chloride group (H + Li group), and a lithium chloride group (Li group) at gestational day 14. Rats received different concentrations of sevoflurane anesthesia for 2 h. The offspring rats were weaned at 28 days for behavioral testing (i.e., Morris Water Maze [MWM]), and fetal brains or postnatal hippocampal tissues were harvested for immunofluorescence staining, real-time PCR, and Western blotting analyses in order to determine the effect of sevoflurane exposure on NSC proliferation and the Wnt/β-catenin signaling pathway. Our results indicated that maternal exposure to 3.5% sevoflurane (H group) during the mid-gestational period impaired the performance of offspring rats in the MWM test, reduced NSC proliferation, and increased protein levels of fetal glycogen synthase kinase-3 beta (GSK-3β). Such treatment also decreased levels of β-catenin protein, CD44 RNA, and Cyclin D1 RNA relative to those observed in the C group. However, these effects were transiently attenuated by treatment with lithium chloride. Conversely, maternal exposure to 2% sevoflurane (L group) did not influence NSC proliferation or the Wnt signaling pathway. Our results suggest that sevoflurane exposure during the second trimester inhibits fetal NSC proliferation via the Wnt/β-catenin pathway and impairs postnatal learning and memory function in a dose-dependent manner. SN - 1095-564X UR - https://www.unboundmedicine.com/medline/citation/29410165/Mid_gestational_sevoflurane_exposure_inhibits_fetal_neural_stem_cell_proliferation_and_impairs_postnatal_learning_and_memory_function_in_a_dose_dependent_manner_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(17)30572-9 DB - PRIME DP - Unbound Medicine ER -