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A novel "dual-potential" ratiometric electrochemiluminescence DNA sensor based on enhancing and quenching effect by G-quadruplex / hemin and Au-Luminol bifunctional nanoparticles.
Biosens Bioelectron. 2018 May 30; 106:64-70.BB

Abstract

An ultrasensitive and stable "dual-potential" ratiometric electrochemiluminescence (ECL) sensor is reported for specific DNA, the femtomolar detection limit (0.12 fM, S/N = 3) and high selectivity insure its potential applications in cancer biomarkers searching or monitoring. The excellent performance of the sensor comes from simultaneously fabricated layer by layer structure "target DNA + Hemin / Au-Luminol NPs / DNA* / sl DNA / TGA / QDs / MWNTs / GCE" mode which was based on the enhancing effect of luminol by G-quadruplex / hemin and Au nanoparticles and the quenching effect of CdSe/ZnS by G-quadruplex / hemin. (i) DNA-SH could combine with Au-Luminol NPs via S-Au bond to solve the problem of poor solubility and weak ECL intensity of luminol in neutral medium. (ii) Target DNA and Hemin formed the G-quadruplex / hemin peroxidase mimicking DNAzyme could enhance the ECL of luminol and quench the ECL of CdSe/ZnS simultaneously. (iii) DNA* was employed to increase a certain distance between CdSe/ZnS and Au-Luminol for enhancing the CdSe/ZnS QDs initial ECL intensity. The dual-potential ratiometric mode lower the influence of background and side reaction of the ECL sensor which were the most important factors in trace sensing.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, PR China.

Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, PR China.

Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, PR China.

Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, PR China.

Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, PR China.

Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, PR China.

Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, PR China.

Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, PR China.

Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, PR China; Tianjin Key Laboratory of Molecular Optoelectronic Science, Department of Chemistry, School of Science, Tianjin University, Tianjin 300072, PR China. Electronic address: luxq@nwnu.edu.cn.

MeSH

Biosensing TechniquesDNADNA, CatalyticG-QuadruplexesGoldHeminHumansLimit of DetectionLuminescent MeasurementsLuminolMetal NanoparticlesPeroxidaseQuantum Dots

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29414090

Citation

Wang, Yanfeng, et al. "A Novel "dual-potential" Ratiometric Electrochemiluminescence DNA Sensor Based On Enhancing and Quenching Effect By G-quadruplex / Hemin and Au-Luminol Bifunctional Nanoparticles." Biosensors & Bioelectronics, vol. 106, 2018, pp. 64-70.

Wang Y, Shan D, Wu G, et al. A novel "dual-potential" ratiometric electrochemiluminescence DNA sensor based on enhancing and quenching effect by G-quadruplex / hemin and Au-Luminol bifunctional nanoparticles. Biosens Bioelectron. 2018;106:64-70.

Wang, Y., Shan, D., Wu, G., Wang, H., Ru, F., Zhang, X., Li, L., Qian, Y., & Lu, X. (2018). A novel "dual-potential" ratiometric electrochemiluminescence DNA sensor based on enhancing and quenching effect by G-quadruplex / hemin and Au-Luminol bifunctional nanoparticles. Biosensors & Bioelectronics, 106, 64-70. https://doi.org/10.1016/j.bios.2018.01.052

Wang Y, et al. A Novel "dual-potential" Ratiometric Electrochemiluminescence DNA Sensor Based On Enhancing and Quenching Effect By G-quadruplex / Hemin and Au-Luminol Bifunctional Nanoparticles. Biosens Bioelectron. 2018 May 30;106:64-70. PubMed PMID: 29414090.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - A novel "dual-potential" ratiometric electrochemiluminescence DNA sensor based on enhancing and quenching effect by G-quadruplex / hemin and Au-Luminol bifunctional nanoparticles. AU - Wang,Yanfeng, AU - Shan,Duoliang, AU - Wu,Guofan, AU - Wang,Huan, AU - Ru,Fan, AU - Zhang,Xiaohui, AU - Li,Linfang, AU - Qian,Yaxuan, AU - Lu,Xiaoquan, Y1 - 2018/01/31/ PY - 2017/11/14/received PY - 2018/01/22/revised PY - 2018/01/23/accepted PY - 2018/2/8/pubmed PY - 2018/8/18/medline PY - 2018/2/8/entrez KW - DNA detection KW - Dual-potential KW - G-quadruplex / hemin KW - Ratiometric SP - 64 EP - 70 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 106 N2 - An ultrasensitive and stable "dual-potential" ratiometric electrochemiluminescence (ECL) sensor is reported for specific DNA, the femtomolar detection limit (0.12 fM, S/N = 3) and high selectivity insure its potential applications in cancer biomarkers searching or monitoring. The excellent performance of the sensor comes from simultaneously fabricated layer by layer structure "target DNA + Hemin / Au-Luminol NPs / DNA* / sl DNA / TGA / QDs / MWNTs / GCE" mode which was based on the enhancing effect of luminol by G-quadruplex / hemin and Au nanoparticles and the quenching effect of CdSe/ZnS by G-quadruplex / hemin. (i) DNA-SH could combine with Au-Luminol NPs via S-Au bond to solve the problem of poor solubility and weak ECL intensity of luminol in neutral medium. (ii) Target DNA and Hemin formed the G-quadruplex / hemin peroxidase mimicking DNAzyme could enhance the ECL of luminol and quench the ECL of CdSe/ZnS simultaneously. (iii) DNA* was employed to increase a certain distance between CdSe/ZnS and Au-Luminol for enhancing the CdSe/ZnS QDs initial ECL intensity. The dual-potential ratiometric mode lower the influence of background and side reaction of the ECL sensor which were the most important factors in trace sensing. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/29414090/A_novel_"dual_potential"_ratiometric_electrochemiluminescence_DNA_sensor_based_on_enhancing_and_quenching_effect_by_G_quadruplex_/_hemin_and_Au_Luminol_bifunctional_nanoparticles_ DB - PRIME DP - Unbound Medicine ER -