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Recombinant buckwheat glutaredoxin intake increases lifespan and stress resistance via hsf-1 upregulation in Caenorhabditis elegans.
Exp Gerontol 2018; 104:86-97EG

Abstract

Glutaredoxin (Grx) is a polypeptide with low molecular weight, which has been extracted from buckwheat and has been suggested to have multiple functions revolving around oxidative stress responses and cell signaling. Here, we report the antioxidant activity of recombinant buckwheat Grx (rbGrx) to reduce aging effects in Caenorhabditis elegans (C. elegans) as well as the mechanism involved. Our results showed that rbGrx beneficially affected the health span of C. elegans, including pharyngeal-pumping rate, locomotion, and lipofuscin accumulation. Furthermore, stress assay showed that rbGrx could extend the lifespan under both oxidative and heat stress. Further studies indicated that the longevity-extending effects of rbGrx could be attributed to its in vitro and in vivo antioxidant activities. After treatment with rbGrx, SOD activity, CAT activity, GSH content, and GSH/GSSG ratio were increased, while MDA content was decreased, which led to low intracellular levels of reactive oxygen species in C. elegans. Moreover, rbGrx up-regulated hsf-1 mRNA level and could not expand the lifespan of the hsf-1 mutant C. elegans (sy441); however, this had no effect on the transcription of daf-16 and skn-1 and could expand the lifespan of both daf-16 and skn-1 mutants. These results suggested dependency of the rbGrx effect on the heat shock transcription factor (HSF-1) and independency on both DAF-16 and SKN-1. In summary, our results demonstrated the anti-aging activity of rbGrx, which increased resistance to cellular stress and improved the health span of C. elegans. These results are very important for the use of rbGrx in anti-aging research.

Authors+Show Affiliations

Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, PR China; Department of oncology, Shanxi Academy of Medical Sciences, Shanxi Dayi Hospital, Taiyuan 030032, PR China.College of Life Science, Shanxi University, Taiyuan 030006, PR China.Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, PR China.College of Life Science, Shanxi University, Taiyuan 030006, PR China.Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, PR China; College of Life Science, Shanxi University, Taiyuan 030006, PR China. Electronic address: zhwang@sxu.edu.cn.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29414672

Citation

Li, Fang, et al. "Recombinant Buckwheat Glutaredoxin Intake Increases Lifespan and Stress Resistance Via Hsf-1 Upregulation in Caenorhabditis Elegans." Experimental Gerontology, vol. 104, 2018, pp. 86-97.
Li F, Ma X, Cui X, et al. Recombinant buckwheat glutaredoxin intake increases lifespan and stress resistance via hsf-1 upregulation in Caenorhabditis elegans. Exp Gerontol. 2018;104:86-97.
Li, F., Ma, X., Cui, X., Li, J., & Wang, Z. (2018). Recombinant buckwheat glutaredoxin intake increases lifespan and stress resistance via hsf-1 upregulation in Caenorhabditis elegans. Experimental Gerontology, 104, pp. 86-97. doi:10.1016/j.exger.2018.01.028.
Li F, et al. Recombinant Buckwheat Glutaredoxin Intake Increases Lifespan and Stress Resistance Via Hsf-1 Upregulation in Caenorhabditis Elegans. Exp Gerontol. 2018;104:86-97. PubMed PMID: 29414672.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recombinant buckwheat glutaredoxin intake increases lifespan and stress resistance via hsf-1 upregulation in Caenorhabditis elegans. AU - Li,Fang, AU - Ma,Xiaoli, AU - Cui,Xiaodong, AU - Li,Jiao, AU - Wang,Zhuanhua, Y1 - 2018/02/04/ PY - 2017/10/17/received PY - 2018/01/12/revised PY - 2018/01/31/accepted PY - 2018/2/8/pubmed PY - 2019/3/21/medline PY - 2018/2/8/entrez KW - Anti-aging KW - Caenorhabditis elegans KW - Heat shock transcription factor KW - Reactive oxygen species KW - Recombinant buckwheat glutaredoxin KW - Stress resistance SP - 86 EP - 97 JF - Experimental gerontology JO - Exp. Gerontol. VL - 104 N2 - Glutaredoxin (Grx) is a polypeptide with low molecular weight, which has been extracted from buckwheat and has been suggested to have multiple functions revolving around oxidative stress responses and cell signaling. Here, we report the antioxidant activity of recombinant buckwheat Grx (rbGrx) to reduce aging effects in Caenorhabditis elegans (C. elegans) as well as the mechanism involved. Our results showed that rbGrx beneficially affected the health span of C. elegans, including pharyngeal-pumping rate, locomotion, and lipofuscin accumulation. Furthermore, stress assay showed that rbGrx could extend the lifespan under both oxidative and heat stress. Further studies indicated that the longevity-extending effects of rbGrx could be attributed to its in vitro and in vivo antioxidant activities. After treatment with rbGrx, SOD activity, CAT activity, GSH content, and GSH/GSSG ratio were increased, while MDA content was decreased, which led to low intracellular levels of reactive oxygen species in C. elegans. Moreover, rbGrx up-regulated hsf-1 mRNA level and could not expand the lifespan of the hsf-1 mutant C. elegans (sy441); however, this had no effect on the transcription of daf-16 and skn-1 and could expand the lifespan of both daf-16 and skn-1 mutants. These results suggested dependency of the rbGrx effect on the heat shock transcription factor (HSF-1) and independency on both DAF-16 and SKN-1. In summary, our results demonstrated the anti-aging activity of rbGrx, which increased resistance to cellular stress and improved the health span of C. elegans. These results are very important for the use of rbGrx in anti-aging research. SN - 1873-6815 UR - https://www.unboundmedicine.com/medline/citation/29414672/Recombinant_buckwheat_glutaredoxin_intake_increases_lifespan_and_stress_resistance_via_hsf_1_upregulation_in_Caenorhabditis_elegans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0531-5565(17)30745-3 DB - PRIME DP - Unbound Medicine ER -