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Enhancement of cell growth and phycocyanin production in Arthrospira (Spirulina) platensis by metabolic stress and nitrate fed-batch.
Bioresour Technol. 2018 May; 255:293-301.BT

Abstract

Arthrospira (Spirulina) platensis is known to have high-quality proteins content and phycocyanin as one of the major pigment constituents of the cells, and the most challenging problem associated with phycocyanin production in Arthrospira is to optimize its intracellular accumulation. The present study evaluated the metabolic stress conditions (by nutrient enrichment) of Arthrospira platensis FACHB-314 for boosting biomass growth and high content phycocyanin accumulation. Experimental results showed that 5 mM sodium glutamate and 7.5 mM succinic acid could enhance biomass yield as well as phycocyanin accumulation compared with that of the control groups. The present study demonstrates that the biomass growth and phycocyanin accumulation were significantly enhanced in fed-batch cultivation of Arthrospira platensis by applying the substrates as metabolic stress agents combined with nitrate feeding strategy. cobA/hemD, hemG and ho genes presented the over-expression level with adding sodium glutamate and succinic acid in cultures, respectively, compared to the control groups.

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Authors+Show Affiliations

Manirafasha E
Department of Chemical and Biochemical Engineering and the Key Lab for Synthetic Biotechnology of Xiamen City, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; University of Rwanda-College of Education, P.O. Box 5039, Kigali, Rwanda.
Murwanashyaka T
Department of Chemical and Biochemical Engineering and the Key Lab for Synthetic Biotechnology of Xiamen City, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Ndikubwimana T
Rwanda Education Board, P.O. Box 3817, Kigali, Rwanda.
Rashid Ahmed N
Department of Chemical and Biochemical Engineering and the Key Lab for Synthetic Biotechnology of Xiamen City, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Liu J
Department of Chemical and Biochemical Engineering and the Key Lab for Synthetic Biotechnology of Xiamen City, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Lu Y
Department of Chemical and Biochemical Engineering and the Key Lab for Synthetic Biotechnology of Xiamen City, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China. Electronic address: ylu@xmu.edu.cn.
Zeng X
College of Energy, Xiamen University, Xiamen 361005, China.
Ling X
Department of Chemical and Biochemical Engineering and the Key Lab for Synthetic Biotechnology of Xiamen City, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Jing K
Department of Chemical and Biochemical Engineering and the Key Lab for Synthetic Biotechnology of Xiamen City, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China. Electronic address: jkj@xmu.edu.cn.

MeSH

BiomassNitratesPhycocyaninSpirulinaStress, Physiological

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29422330

Citation

Manirafasha, Emmanuel, et al. "Enhancement of Cell Growth and Phycocyanin Production in Arthrospira (Spirulina) Platensis By Metabolic Stress and Nitrate Fed-batch." Bioresource Technology, vol. 255, 2018, pp. 293-301.
Manirafasha E, Murwanashyaka T, Ndikubwimana T, et al. Enhancement of cell growth and phycocyanin production in Arthrospira (Spirulina) platensis by metabolic stress and nitrate fed-batch. Bioresour Technol. 2018;255:293-301.
Manirafasha, E., Murwanashyaka, T., Ndikubwimana, T., Rashid Ahmed, N., Liu, J., Lu, Y., Zeng, X., Ling, X., & Jing, K. (2018). Enhancement of cell growth and phycocyanin production in Arthrospira (Spirulina) platensis by metabolic stress and nitrate fed-batch. Bioresource Technology, 255, 293-301. https://doi.org/10.1016/j.biortech.2017.12.068
Manirafasha E, et al. Enhancement of Cell Growth and Phycocyanin Production in Arthrospira (Spirulina) Platensis By Metabolic Stress and Nitrate Fed-batch. Bioresour Technol. 2018;255:293-301. PubMed PMID: 29422330.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhancement of cell growth and phycocyanin production in Arthrospira (Spirulina) platensis by metabolic stress and nitrate fed-batch. AU - Manirafasha,Emmanuel, AU - Murwanashyaka,Theophile, AU - Ndikubwimana,Theoneste, AU - Rashid Ahmed,Nur, AU - Liu,Jingyi, AU - Lu,Yinghua, AU - Zeng,Xianhai, AU - Ling,Xueping, AU - Jing,Keju, Y1 - 2017/12/23/ PY - 2017/11/01/received PY - 2017/12/20/revised PY - 2017/12/21/accepted PY - 2018/2/10/pubmed PY - 2018/6/2/medline PY - 2018/2/10/entrez KW - Arthrospira platensis KW - Fed-batch cultivation KW - Metabolic stress KW - Phycocyanin KW - Sodium glutamate KW - Succinic acid SP - 293 EP - 301 JF - Bioresource technology JO - Bioresour Technol VL - 255 N2 - Arthrospira (Spirulina) platensis is known to have high-quality proteins content and phycocyanin as one of the major pigment constituents of the cells, and the most challenging problem associated with phycocyanin production in Arthrospira is to optimize its intracellular accumulation. The present study evaluated the metabolic stress conditions (by nutrient enrichment) of Arthrospira platensis FACHB-314 for boosting biomass growth and high content phycocyanin accumulation. Experimental results showed that 5 mM sodium glutamate and 7.5 mM succinic acid could enhance biomass yield as well as phycocyanin accumulation compared with that of the control groups. The present study demonstrates that the biomass growth and phycocyanin accumulation were significantly enhanced in fed-batch cultivation of Arthrospira platensis by applying the substrates as metabolic stress agents combined with nitrate feeding strategy. cobA/hemD, hemG and ho genes presented the over-expression level with adding sodium glutamate and succinic acid in cultures, respectively, compared to the control groups. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/29422330/Enhancement_of_cell_growth_and_phycocyanin_production_in_Arthrospira__Spirulina__platensis_by_metabolic_stress_and_nitrate_fed_batch_ DB - PRIME DP - Unbound Medicine ER -