Tags

Type your tag names separated by a space and hit enter

Involvement of spinal cannabinoid receptors in the antipruritic effects of WIN 55,212-2, a cannabinoid receptor agonist.
Clin Exp Dermatol. 2018 Jul; 43(5):553-558.CE

Abstract

BACKGROUND

Cannabinoids have been used for their analgesic and euphoric effects for millennia, but recently the antipruritic effects of cannabis have been discovered. Considering the similarities between pain and itch sensations, we hypothesized that cannabinoid receptors may play a role in the antipruritic effects of cannabinoids.

AIM

To analyse the role of the spinal cannabinoid receptors, CB1 and CB2, in the antipruritic effects of the cannabinoid agonist WIN 55,212-2.

METHODS

Male Balb/c mice weighing 20-30 g were used. Scratching behaviour in the mice was produced by injection of serotonin 5 μg/50 μL intradermally into the nape of the neck. Scratching of the site of injection by the hind paws was video-recorded for 30 min. After testing different doses of WIN 55,212-2 [1, 3 and 10 mg/kg intraperitoneally (IP)], the effects of the CB1 receptor antagonist, AM-251 [1 μg/mouse administered intrathecally (IT)] and the CB2 receptor antagonist AM-630 (4 μg/mouse IT) on the antipruritic effects of WIN 55,212-2 were studied using a rotarod apparatus.

RESULTS

WIN 55,212-2 (1, 3 or 10 mg/kg IP) dose-dependently decreased serotonin-induced scratches. The receptor antagonist CB1 partially reversed the effects of WIN 55,212-2 (P < 0.05); whereas CB2 had no statistically significant effect. WIN 55,212-2 impaired motor function only at the highest dose given (10 mg/kg, P < 0.05).

CONCLUSIONS

Our findings support prior researches indicating that cannabinoids exert antipruritic effects. Moreover, our results show that the antipruritic effects of cannabinoids are partially mediated by spinal CB1 receptors.

Authors+Show Affiliations

Department of Medical Pharmacology, Faculty of Medicine, Trakya University, Edirne, Turkey.Department of Medical Pharmacology, Faculty of Medicine, Trakya University, Edirne, Turkey.Department of Medical Pharmacology, Faculty of Medicine, Trakya University, Edirne, Turkey.Department of Medical Pharmacology, Faculty of Medicine, Trakya University, Edirne, Turkey.Department of Medical Pharmacology, Faculty of Medicine, Trakya University, Edirne, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29424035

Citation

Bilir, K A., et al. "Involvement of Spinal Cannabinoid Receptors in the Antipruritic Effects of WIN 55,212-2, a Cannabinoid Receptor Agonist." Clinical and Experimental Dermatology, vol. 43, no. 5, 2018, pp. 553-558.
Bilir KA, Anli G, Ozkan E, et al. Involvement of spinal cannabinoid receptors in the antipruritic effects of WIN 55,212-2, a cannabinoid receptor agonist. Clin Exp Dermatol. 2018;43(5):553-558.
Bilir, K. A., Anli, G., Ozkan, E., Gunduz, O., & Ulugol, A. (2018). Involvement of spinal cannabinoid receptors in the antipruritic effects of WIN 55,212-2, a cannabinoid receptor agonist. Clinical and Experimental Dermatology, 43(5), 553-558. https://doi.org/10.1111/ced.13398
Bilir KA, et al. Involvement of Spinal Cannabinoid Receptors in the Antipruritic Effects of WIN 55,212-2, a Cannabinoid Receptor Agonist. Clin Exp Dermatol. 2018;43(5):553-558. PubMed PMID: 29424035.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of spinal cannabinoid receptors in the antipruritic effects of WIN 55,212-2, a cannabinoid receptor agonist. AU - Bilir,K A, AU - Anli,G, AU - Ozkan,E, AU - Gunduz,O, AU - Ulugol,A, Y1 - 2018/02/09/ PY - 2017/07/26/accepted PY - 2018/2/10/pubmed PY - 2018/11/9/medline PY - 2018/2/10/entrez SP - 553 EP - 558 JF - Clinical and experimental dermatology JO - Clin Exp Dermatol VL - 43 IS - 5 N2 - BACKGROUND: Cannabinoids have been used for their analgesic and euphoric effects for millennia, but recently the antipruritic effects of cannabis have been discovered. Considering the similarities between pain and itch sensations, we hypothesized that cannabinoid receptors may play a role in the antipruritic effects of cannabinoids. AIM: To analyse the role of the spinal cannabinoid receptors, CB1 and CB2, in the antipruritic effects of the cannabinoid agonist WIN 55,212-2. METHODS: Male Balb/c mice weighing 20-30 g were used. Scratching behaviour in the mice was produced by injection of serotonin 5 μg/50 μL intradermally into the nape of the neck. Scratching of the site of injection by the hind paws was video-recorded for 30 min. After testing different doses of WIN 55,212-2 [1, 3 and 10 mg/kg intraperitoneally (IP)], the effects of the CB1 receptor antagonist, AM-251 [1 μg/mouse administered intrathecally (IT)] and the CB2 receptor antagonist AM-630 (4 μg/mouse IT) on the antipruritic effects of WIN 55,212-2 were studied using a rotarod apparatus. RESULTS: WIN 55,212-2 (1, 3 or 10 mg/kg IP) dose-dependently decreased serotonin-induced scratches. The receptor antagonist CB1 partially reversed the effects of WIN 55,212-2 (P < 0.05); whereas CB2 had no statistically significant effect. WIN 55,212-2 impaired motor function only at the highest dose given (10 mg/kg, P < 0.05). CONCLUSIONS: Our findings support prior researches indicating that cannabinoids exert antipruritic effects. Moreover, our results show that the antipruritic effects of cannabinoids are partially mediated by spinal CB1 receptors. SN - 1365-2230 UR - https://www.unboundmedicine.com/medline/citation/29424035/Involvement_of_spinal_cannabinoid_receptors_in_the_antipruritic_effects_of_WIN_55212_2_a_cannabinoid_receptor_agonist_ L2 - https://doi.org/10.1111/ced.13398 DB - PRIME DP - Unbound Medicine ER -