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Antinociceptive effects of the endogenous cannabinoid peptide agonist VD-hemopressin(β) in mice.
Brain Res Bull. 2018 05; 139:48-55.BR

Abstract

Cannabinoids (CBs) play important roles in pain modulation. Recently, VD-hemopressin(β) [VD-Hpβ], a 12-residue β-hemoglobin-derived peptide, was reported to activate both CB1 and CB2 receptors in vitro. To further characterize in vivo actions of VD-Hpβ, its antinociceptive activity and site(s) were evaluated in the mouse tail-flick test, and supraspinal antinociception of VD-Hpβ was further assessed in the writhing test. Our results demonstrated that supraspinal, intrathecal, subcutaneous and intraperitoneal administrations of VD-Hpβ produced analgesia in the tail-flick test. When given at the same levels, the CB1 antagonist AM251, rather than the CB2 antagonist AM630 diminished VD-Hpβ-induced antinociception. Furthermore, our results indicated that supraspinal, intrathecal or subcutaneous pretreatment with AM251 significantly inhibited VD-Hpβ-induced systemic antinociception. In the writhing test, supraspinal VD-Hpβ inhibited pain-related behaviors, which was partially prevented by AM251. Notably, supraspinal administration of VD-Hpβ failed to affect motor function at the antinociceptive doses. These findings suggest that VD-Hpβ induces CB1 receptor-mediated antinociception in tail-flick test in various routes of administration, and its systemic antinociception is mediated by both central and peripheral CB1 receptor. In addition, VD-Hpβ produces analgesic activity in the writhing test, which is at least partially mediated by CB1 receptor. Therefore, our present animal models show a CB1 agonistic character of VD-Hpβ, an endogenous cannabinoid peptide.

Authors+Show Affiliations

Key Laboratory of Preclinical Study for New Drugs of Gansu Province, and Institute of Physiology, School of Basic Medical Sciences, Lanzhou University, 199 Donggang West Road, Lanzhou, 730000, China.Key Laboratory of Preclinical Study for New Drugs of Gansu Province, and Institute of Physiology, School of Basic Medical Sciences, Lanzhou University, 199 Donggang West Road, Lanzhou, 730000, China.Key Laboratory of Preclinical Study for New Drugs of Gansu Province, and Institute of Physiology, School of Basic Medical Sciences, Lanzhou University, 199 Donggang West Road, Lanzhou, 730000, China.Key Laboratory of Preclinical Study for New Drugs of Gansu Province, and Institute of Physiology, School of Basic Medical Sciences, Lanzhou University, 199 Donggang West Road, Lanzhou, 730000, China.Key Laboratory of Preclinical Study for New Drugs of Gansu Province, and Institute of Physiology, School of Basic Medical Sciences, Lanzhou University, 199 Donggang West Road, Lanzhou, 730000, China.Key Laboratory of Preclinical Study for New Drugs of Gansu Province, and Institute of Physiology, School of Basic Medical Sciences, Lanzhou University, 199 Donggang West Road, Lanzhou, 730000, China.Key Laboratory of Preclinical Study for New Drugs of Gansu Province, and Institute of Physiology, School of Basic Medical Sciences, Lanzhou University, 199 Donggang West Road, Lanzhou, 730000, China.Key Laboratory of Preclinical Study for New Drugs of Gansu Province, and Institute of Physiology, School of Basic Medical Sciences, Lanzhou University, 199 Donggang West Road, Lanzhou, 730000, China.Key Laboratory of Preclinical Study for New Drugs of Gansu Province, and Institute of Physiology, School of Basic Medical Sciences, Lanzhou University, 199 Donggang West Road, Lanzhou, 730000, China.Key Laboratory of Preclinical Study for New Drugs of Gansu Province, and Institute of Physiology, School of Basic Medical Sciences, Lanzhou University, 199 Donggang West Road, Lanzhou, 730000, China. Electronic address: fangq@lzu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29425797

Citation

Wang, Pei, et al. "Antinociceptive Effects of the Endogenous Cannabinoid Peptide Agonist VD-hemopressin(β) in Mice." Brain Research Bulletin, vol. 139, 2018, pp. 48-55.
Wang P, Zheng T, Zhang M, et al. Antinociceptive effects of the endogenous cannabinoid peptide agonist VD-hemopressin(β) in mice. Brain Res Bull. 2018;139:48-55.
Wang, P., Zheng, T., Zhang, M., Xu, B., Zhang, R., Zhang, T., Zhao, W., Shi, X., Zhang, Q., & Fang, Q. (2018). Antinociceptive effects of the endogenous cannabinoid peptide agonist VD-hemopressin(β) in mice. Brain Research Bulletin, 139, 48-55. https://doi.org/10.1016/j.brainresbull.2018.02.003
Wang P, et al. Antinociceptive Effects of the Endogenous Cannabinoid Peptide Agonist VD-hemopressin(β) in Mice. Brain Res Bull. 2018;139:48-55. PubMed PMID: 29425797.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antinociceptive effects of the endogenous cannabinoid peptide agonist VD-hemopressin(β) in mice. AU - Wang,Pei, AU - Zheng,Ting, AU - Zhang,Mengna, AU - Xu,Biao, AU - Zhang,Run, AU - Zhang,Ting, AU - Zhao,Weidong, AU - Shi,Xuerui, AU - Zhang,Qinqin, AU - Fang,Quan, Y1 - 2018/02/06/ PY - 2017/12/17/received PY - 2018/01/30/revised PY - 2018/02/02/accepted PY - 2018/2/10/pubmed PY - 2019/3/13/medline PY - 2018/2/10/entrez KW - Antinociception KW - Cannabinoid KW - Cannabinoid receptor type 1 (CB(1)) KW - Mice KW - VD-hemopressin(β) SP - 48 EP - 55 JF - Brain research bulletin JO - Brain Res Bull VL - 139 N2 - Cannabinoids (CBs) play important roles in pain modulation. Recently, VD-hemopressin(β) [VD-Hpβ], a 12-residue β-hemoglobin-derived peptide, was reported to activate both CB1 and CB2 receptors in vitro. To further characterize in vivo actions of VD-Hpβ, its antinociceptive activity and site(s) were evaluated in the mouse tail-flick test, and supraspinal antinociception of VD-Hpβ was further assessed in the writhing test. Our results demonstrated that supraspinal, intrathecal, subcutaneous and intraperitoneal administrations of VD-Hpβ produced analgesia in the tail-flick test. When given at the same levels, the CB1 antagonist AM251, rather than the CB2 antagonist AM630 diminished VD-Hpβ-induced antinociception. Furthermore, our results indicated that supraspinal, intrathecal or subcutaneous pretreatment with AM251 significantly inhibited VD-Hpβ-induced systemic antinociception. In the writhing test, supraspinal VD-Hpβ inhibited pain-related behaviors, which was partially prevented by AM251. Notably, supraspinal administration of VD-Hpβ failed to affect motor function at the antinociceptive doses. These findings suggest that VD-Hpβ induces CB1 receptor-mediated antinociception in tail-flick test in various routes of administration, and its systemic antinociception is mediated by both central and peripheral CB1 receptor. In addition, VD-Hpβ produces analgesic activity in the writhing test, which is at least partially mediated by CB1 receptor. Therefore, our present animal models show a CB1 agonistic character of VD-Hpβ, an endogenous cannabinoid peptide. SN - 1873-2747 UR - https://www.unboundmedicine.com/medline/citation/29425797/Antinociceptive_effects_of_the_endogenous_cannabinoid_peptide_agonist_VD_hemopressin_β__in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0361-9230(17)30762-1 DB - PRIME DP - Unbound Medicine ER -