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Identification of the WRKY gene family and functional analysis of two genes in Caragana intermedia.
BMC Plant Biol. 2018 02 09; 18(1):31.BP

Abstract

BACKGROUND

WRKY transcription factors, one of the largest families of transcriptional regulators in plants, play important roles in plant development and various stress responses. The WRKYs of Caragana intermedia are still not well characterized, although many WRKYs have been identified in various plant species.

RESULTS

We identified 53 CiWRKY genes from C. intermedia transcriptome data, 28 of which exhibited complete open reading frames (ORFs). These CiWRKYs were divided into three groups via phylogenetic analysis according to their WRKY domains and zinc finger motifs. Conserved domain analysis showed that the CiWRKY proteins contain a highly conserved WRKYGQK motif and two variant motifs (WRKYGKK and WKKYEEK). The subcellular localization of CiWRKY26 and CiWRKY28-1 indicated that these two proteins localized exclusively to nuclei, supporting their role as transcription factors. The expression patterns of the 28 CiWRKYs with complete ORFs were examined through quantitative real-time PCR (qRT-PCR) in various tissues and under different abiotic stresses (drought, cold, salt, high-pH and abscisic acid (ABA)). The results showed that each CiWRKY responded to at least one stress treatment. Furthermore, overexpression of CiWRKY75-1 and CiWRKY40-4 in Arabidopsis thaliana suppressed the drought stress tolerance of the plants and delayed leaf senescence, respectively.

CONCLUSIONS

Fifty-three CiWRKY genes from the C. intermedia transcriptome were identified and divided into three groups via phylogenetic analysis. The expression patterns of the 28 CiWRKYs under different abiotic stresses suggested that each CiWRKY responded to at least one stress treatment. Overexpression of CiWRKY75-1 and CiWRKY40-4 suppressed the drought stress tolerance of Arabidopsis and delayed leaf senescence, respectively. These results provide a basis for the molecular mechanism through which CiWRKYs mediate stress tolerance.

Authors+Show Affiliations

College of Life Sciences, Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology, Inner Mongolia Agricultural University, Hohhot, China.College of Life Sciences, Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology, Inner Mongolia Agricultural University, Hohhot, China.Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Key Laboratory of Grassland Ecology and Restoration, Ministry of Agriculture, Hohhot, China.College of Life Sciences, Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology, Inner Mongolia Agricultural University, Hohhot, China.College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, China.College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, China.College of Life Sciences, Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology, Inner Mongolia Agricultural University, Hohhot, China.College of Life Sciences, Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology, Inner Mongolia Agricultural University, Hohhot, China. wangruigang@imau.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29426284

Citation

Wan, Yongqing, et al. "Identification of the WRKY Gene Family and Functional Analysis of Two Genes in Caragana Intermedia." BMC Plant Biology, vol. 18, no. 1, 2018, p. 31.
Wan Y, Mao M, Wan D, et al. Identification of the WRKY gene family and functional analysis of two genes in Caragana intermedia. BMC Plant Biol. 2018;18(1):31.
Wan, Y., Mao, M., Wan, D., Yang, Q., Yang, F., Mandlaa, ., Li, G., & Wang, R. (2018). Identification of the WRKY gene family and functional analysis of two genes in Caragana intermedia. BMC Plant Biology, 18(1), 31. https://doi.org/10.1186/s12870-018-1235-3
Wan Y, et al. Identification of the WRKY Gene Family and Functional Analysis of Two Genes in Caragana Intermedia. BMC Plant Biol. 2018 02 9;18(1):31. PubMed PMID: 29426284.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of the WRKY gene family and functional analysis of two genes in Caragana intermedia. AU - Wan,Yongqing, AU - Mao,Mingzhu, AU - Wan,Dongli, AU - Yang,Qi, AU - Yang,Feiyun, AU - Mandlaa,, AU - Li,Guojing, AU - Wang,Ruigang, Y1 - 2018/02/09/ PY - 2017/07/11/received PY - 2018/01/14/accepted PY - 2018/2/11/entrez PY - 2018/2/11/pubmed PY - 2018/8/8/medline KW - Abiotic stress KW - Caragana Intermedia KW - Function analysis KW - Gene expression KW - Subcellular localization KW - WRKY gene family SP - 31 EP - 31 JF - BMC plant biology JO - BMC Plant Biol VL - 18 IS - 1 N2 - BACKGROUND: WRKY transcription factors, one of the largest families of transcriptional regulators in plants, play important roles in plant development and various stress responses. The WRKYs of Caragana intermedia are still not well characterized, although many WRKYs have been identified in various plant species. RESULTS: We identified 53 CiWRKY genes from C. intermedia transcriptome data, 28 of which exhibited complete open reading frames (ORFs). These CiWRKYs were divided into three groups via phylogenetic analysis according to their WRKY domains and zinc finger motifs. Conserved domain analysis showed that the CiWRKY proteins contain a highly conserved WRKYGQK motif and two variant motifs (WRKYGKK and WKKYEEK). The subcellular localization of CiWRKY26 and CiWRKY28-1 indicated that these two proteins localized exclusively to nuclei, supporting their role as transcription factors. The expression patterns of the 28 CiWRKYs with complete ORFs were examined through quantitative real-time PCR (qRT-PCR) in various tissues and under different abiotic stresses (drought, cold, salt, high-pH and abscisic acid (ABA)). The results showed that each CiWRKY responded to at least one stress treatment. Furthermore, overexpression of CiWRKY75-1 and CiWRKY40-4 in Arabidopsis thaliana suppressed the drought stress tolerance of the plants and delayed leaf senescence, respectively. CONCLUSIONS: Fifty-three CiWRKY genes from the C. intermedia transcriptome were identified and divided into three groups via phylogenetic analysis. The expression patterns of the 28 CiWRKYs under different abiotic stresses suggested that each CiWRKY responded to at least one stress treatment. Overexpression of CiWRKY75-1 and CiWRKY40-4 suppressed the drought stress tolerance of Arabidopsis and delayed leaf senescence, respectively. These results provide a basis for the molecular mechanism through which CiWRKYs mediate stress tolerance. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/29426284/Identification_of_the_WRKY_gene_family_and_functional_analysis_of_two_genes_in_Caragana_intermedia_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-018-1235-3 DB - PRIME DP - Unbound Medicine ER -