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Effect of dietary fish oil supplements alone or in combination with sunflower and linseed oil on ruminal lipid metabolism and bacterial populations in lactating cows.
J Dairy Sci 2018; 101(4):3021-3035JD

Abstract

Fish oil (FO) alters ruminal biohydrogenation causing trans fatty acid (FA) intermediates to accumulate, but the effects of 18-carbon polyunsaturated FA supply on ruminal long-chain FA metabolism and microbial communities in cattle fed FO are not well established. Four cows fitted with rumen cannula were used in a 4 × 4 Latin square with 21-d experimental periods to evaluate the effects of FO alone or in combination with plant oils high in 18:2n-6 or 18:3n-3 on rumen microbial ecology and flow of FA at the omasum. Treatments comprised a basal grass silage-based diet containing no additional oil (control) or supplements of FO (200 g/d) or FO (200 g/d) plus 500 g/d of sunflower oil (SFO) or linseed oil (LFO). Flow of FA was determined using the omasal sampling technique. The relative abundance of key biohydrogenating bacteria was assessed by quantitative PCR on 16S rRNA genes in omasal digesta. Fish oil-supplemented treatments increased the amounts of trans-18:1, trans-18:2, and 20- to 22-carbon polyunsaturated FA escaping the rumen. Relative to the control, oil supplements had no effect on the amount of 18:0 leaving the rumen, but LFO decreased the flow of 18:0 at the omasum compared with SFO. Both SFO and LFO increased trans-18:1 relative to FO, whereas LFO resulted in the highest trans-18:2 and 20- to 22-carbon FA flow. Supplements of FO plus plant oils shifted biohydrogenation toward trans-10 18:1 formation. Compared with FO alone, the ruminal metabolism of 22:6n-3 in the rumen of lactating cows is more extensive on diets containing higher amounts of 18-carbon polyunsaturated FA. However, the biohydrogenation of 22:5n-3 was less extensive in LFO than SFO, but showed no difference between FO and diets containing plant oils. Ruminal outflow of 20:5n-3 was not altered when plant oils were added to FO. Alterations in the amount of intermediates at the omasum or ruminal biohydrogenation pathways were not accompanied by major changes in analyzed bacterial populations. In conclusion, dietary supplements of FO alone or in combination with plant oils increase the amount of biohydrogenation intermediates containing 1 or more trans double bonds escaping the rumen, which may have implications for host metabolism and the nutritional quality of ruminant foods.

Authors+Show Affiliations

Milk Production Solutions, Green Technology, Natural Resources Institute Finland (Luke), Jokioinen, FI-31600, Finland. Electronic address: piia.kairenius@luke.fi.Milk Production Solutions, Green Technology, Natural Resources Institute Finland (Luke), Jokioinen, FI-31600, Finland.Milk Production Solutions, Green Technology, Natural Resources Institute Finland (Luke), Jokioinen, FI-31600, Finland.AgResearch Ltd., Grasslands Research Centre, Private Bag 11-008, Palmerston North 4442, New Zealand.Milk Production Solutions, Green Technology, Natural Resources Institute Finland (Luke), Jokioinen, FI-31600, Finland.Department of Agricultural Sciences, University of Helsinki, PO Box 28, FI 00014, Finland.Milk Production Solutions, Green Technology, Natural Resources Institute Finland (Luke), Jokioinen, FI-31600, Finland; Department of Agriculture in Northern Sweden, Swedish University of Agricultural Sciences, S-90183, Umeå, Sweden.Rowett Institute of Nutrition and Health, University of Aberdeen, Bucksburn, Aberdeen, AB21 9SB, United Kingdom.Milk Production Solutions, Green Technology, Natural Resources Institute Finland (Luke), Jokioinen, FI-31600, Finland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29428753

Citation

Kairenius, P, et al. "Effect of Dietary Fish Oil Supplements Alone or in Combination With Sunflower and Linseed Oil On Ruminal Lipid Metabolism and Bacterial Populations in Lactating Cows." Journal of Dairy Science, vol. 101, no. 4, 2018, pp. 3021-3035.
Kairenius P, Leskinen H, Toivonen V, et al. Effect of dietary fish oil supplements alone or in combination with sunflower and linseed oil on ruminal lipid metabolism and bacterial populations in lactating cows. J Dairy Sci. 2018;101(4):3021-3035.
Kairenius, P., Leskinen, H., Toivonen, V., Muetzel, S., Ahvenjärvi, S., Vanhatalo, A., ... Shingfield, K. J. (2018). Effect of dietary fish oil supplements alone or in combination with sunflower and linseed oil on ruminal lipid metabolism and bacterial populations in lactating cows. Journal of Dairy Science, 101(4), pp. 3021-3035. doi:10.3168/jds.2017-13776.
Kairenius P, et al. Effect of Dietary Fish Oil Supplements Alone or in Combination With Sunflower and Linseed Oil On Ruminal Lipid Metabolism and Bacterial Populations in Lactating Cows. J Dairy Sci. 2018;101(4):3021-3035. PubMed PMID: 29428753.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of dietary fish oil supplements alone or in combination with sunflower and linseed oil on ruminal lipid metabolism and bacterial populations in lactating cows. AU - Kairenius,P, AU - Leskinen,H, AU - Toivonen,V, AU - Muetzel,S, AU - Ahvenjärvi,S, AU - Vanhatalo,A, AU - Huhtanen,P, AU - Wallace,R J, AU - Shingfield,K J, Y1 - 2018/02/07/ PY - 2017/09/01/received PY - 2017/12/14/accepted PY - 2018/2/13/pubmed PY - 2018/8/28/medline PY - 2018/2/12/entrez KW - Butyrivibrio KW - biohydrogenation KW - plant oil KW - polyunsaturated fatty acid SP - 3021 EP - 3035 JF - Journal of dairy science JO - J. Dairy Sci. VL - 101 IS - 4 N2 - Fish oil (FO) alters ruminal biohydrogenation causing trans fatty acid (FA) intermediates to accumulate, but the effects of 18-carbon polyunsaturated FA supply on ruminal long-chain FA metabolism and microbial communities in cattle fed FO are not well established. Four cows fitted with rumen cannula were used in a 4 × 4 Latin square with 21-d experimental periods to evaluate the effects of FO alone or in combination with plant oils high in 18:2n-6 or 18:3n-3 on rumen microbial ecology and flow of FA at the omasum. Treatments comprised a basal grass silage-based diet containing no additional oil (control) or supplements of FO (200 g/d) or FO (200 g/d) plus 500 g/d of sunflower oil (SFO) or linseed oil (LFO). Flow of FA was determined using the omasal sampling technique. The relative abundance of key biohydrogenating bacteria was assessed by quantitative PCR on 16S rRNA genes in omasal digesta. Fish oil-supplemented treatments increased the amounts of trans-18:1, trans-18:2, and 20- to 22-carbon polyunsaturated FA escaping the rumen. Relative to the control, oil supplements had no effect on the amount of 18:0 leaving the rumen, but LFO decreased the flow of 18:0 at the omasum compared with SFO. Both SFO and LFO increased trans-18:1 relative to FO, whereas LFO resulted in the highest trans-18:2 and 20- to 22-carbon FA flow. Supplements of FO plus plant oils shifted biohydrogenation toward trans-10 18:1 formation. Compared with FO alone, the ruminal metabolism of 22:6n-3 in the rumen of lactating cows is more extensive on diets containing higher amounts of 18-carbon polyunsaturated FA. However, the biohydrogenation of 22:5n-3 was less extensive in LFO than SFO, but showed no difference between FO and diets containing plant oils. Ruminal outflow of 20:5n-3 was not altered when plant oils were added to FO. Alterations in the amount of intermediates at the omasum or ruminal biohydrogenation pathways were not accompanied by major changes in analyzed bacterial populations. In conclusion, dietary supplements of FO alone or in combination with plant oils increase the amount of biohydrogenation intermediates containing 1 or more trans double bonds escaping the rumen, which may have implications for host metabolism and the nutritional quality of ruminant foods. SN - 1525-3198 UR - https://www.unboundmedicine.com/medline/citation/29428753/Effect_of_dietary_fish_oil_supplements_alone_or_in_combination_with_sunflower_and_linseed_oil_on_ruminal_lipid_metabolism_and_bacterial_populations_in_lactating_cows_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(18)30093-6 DB - PRIME DP - Unbound Medicine ER -