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Extraction optimization, total phenolic, flavonoid contents, HPLC-DAD analysis and diverse pharmacological evaluations of Dysphania ambrosioides (L.) Mosyakin & Clemants.
Nat Prod Res. 2019 Jan; 33(1):136-142.NP

Abstract

The present study aims to evaluate phytochemical and pharmacological potentials of Dysphania ambrosioides (L.) Mosyakin & Clemants previously known as Chenopodium ambrosioides L. Extraction was carried out using 14 solvents with wide range of polarity to find out the best solvent system for each bioactivity. Total phenolic and flavonoids contents were measured colorimetrically and polyphenolics were quantified via HPLC-DAD analysis. The samples were screened for inhibitory potentials against free radicals, leishmania, cancer cell lines, protein kinase, α-Amylase enzymes and microbial strains. Among all solvents, maximum percentage of extract was recovered from methanol-water fraction of leaves. HPLC analysis exhibited the presence of rutin, myricetin and quercetin. In DPPH assay, methanolic leaf extract exhibited IC50 value of 130.7 ± 0.57 μg/mL. Considerable α-amylase inhibitory, cytotoxic, leishmanicidal and antimicrobial potentials were exhibited by plant samples. D. ambrosioides revealed significant antioxidant, cytotoxic, antimicrobial and anti-diabetic potentials and thus warrant further detailed studies to find novel drugs.

Authors+Show Affiliations

a Department of Biotechnology , Quaid-i-Azam University , Islamabad , Pakistan.b Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety , CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology , Beijing , China.c Department of Eastern Medicine and Surgery , Qarshi University , Lahore , Pakistan.a Department of Biotechnology , Quaid-i-Azam University , Islamabad , Pakistan.d Department of Pharmacy , University of Malakand , Khyber Pakhtoonkhwa , Pakistan.a Department of Biotechnology , Quaid-i-Azam University , Islamabad , Pakistan. c Department of Eastern Medicine and Surgery , Qarshi University , Lahore , Pakistan. e Pakistan Academy of Sciences , Islamabad , Pakistan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29430965

Citation

Zohra, Tanzeel, et al. "Extraction Optimization, Total Phenolic, Flavonoid Contents, HPLC-DAD Analysis and Diverse Pharmacological Evaluations of Dysphania Ambrosioides (L.) Mosyakin & Clemants." Natural Product Research, vol. 33, no. 1, 2019, pp. 136-142.
Zohra T, Ovais M, Khalil AT, et al. Extraction optimization, total phenolic, flavonoid contents, HPLC-DAD analysis and diverse pharmacological evaluations of Dysphania ambrosioides (L.) Mosyakin & Clemants. Nat Prod Res. 2019;33(1):136-142.
Zohra, T., Ovais, M., Khalil, A. T., Qasim, M., Ayaz, M., & Shinwari, Z. K. (2019). Extraction optimization, total phenolic, flavonoid contents, HPLC-DAD analysis and diverse pharmacological evaluations of Dysphania ambrosioides (L.) Mosyakin & Clemants. Natural Product Research, 33(1), 136-142. https://doi.org/10.1080/14786419.2018.1437428
Zohra T, et al. Extraction Optimization, Total Phenolic, Flavonoid Contents, HPLC-DAD Analysis and Diverse Pharmacological Evaluations of Dysphania Ambrosioides (L.) Mosyakin & Clemants. Nat Prod Res. 2019;33(1):136-142. PubMed PMID: 29430965.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extraction optimization, total phenolic, flavonoid contents, HPLC-DAD analysis and diverse pharmacological evaluations of Dysphania ambrosioides (L.) Mosyakin & Clemants. AU - Zohra,Tanzeel, AU - Ovais,Muhammad, AU - Khalil,Ali Talha, AU - Qasim,Muhammad, AU - Ayaz,Muhammad, AU - Shinwari,Zabta Khan, Y1 - 2018/02/12/ PY - 2018/2/13/pubmed PY - 2019/5/16/medline PY - 2018/2/13/entrez KW - L. KW - HPLC-DAD KW - HepG2 cell line KW - antimicrobial resistance KW - antioxidants KW - cytotoxicity KW - leishmania KW - protein kinase SP - 136 EP - 142 JF - Natural product research JO - Nat Prod Res VL - 33 IS - 1 N2 - The present study aims to evaluate phytochemical and pharmacological potentials of Dysphania ambrosioides (L.) Mosyakin & Clemants previously known as Chenopodium ambrosioides L. Extraction was carried out using 14 solvents with wide range of polarity to find out the best solvent system for each bioactivity. Total phenolic and flavonoids contents were measured colorimetrically and polyphenolics were quantified via HPLC-DAD analysis. The samples were screened for inhibitory potentials against free radicals, leishmania, cancer cell lines, protein kinase, α-Amylase enzymes and microbial strains. Among all solvents, maximum percentage of extract was recovered from methanol-water fraction of leaves. HPLC analysis exhibited the presence of rutin, myricetin and quercetin. In DPPH assay, methanolic leaf extract exhibited IC50 value of 130.7 ± 0.57 μg/mL. Considerable α-amylase inhibitory, cytotoxic, leishmanicidal and antimicrobial potentials were exhibited by plant samples. D. ambrosioides revealed significant antioxidant, cytotoxic, antimicrobial and anti-diabetic potentials and thus warrant further detailed studies to find novel drugs. SN - 1478-6427 UR - https://www.unboundmedicine.com/medline/citation/29430965/Extraction_optimization_total_phenolic_flavonoid_contents_HPLC_DAD_analysis_and_diverse_pharmacological_evaluations_of_Dysphania_ambrosioides__L___Mosyakin_&_Clemants_ DB - PRIME DP - Unbound Medicine ER -