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Intraspecific variability of ciprofloxacin accumulation, tolerance, and metabolism in Chinese flowering cabbage (Brassica parachinensis).
J Hazard Mater. 2018 05 05; 349:252-261.JH

Abstract

To investigate the mechanism of genotype differences in ciprofloxacin (CIP) accumulation, this study was designed to compare the tolerance and metabolic responses to CIP exposure between low (Cutai) and high (Sijiu) CIP-accumulation cultivars of Brassica parachinensis. Decreases in biomass and chlorophyll content were significantly greater (p < 0.05) and toxicities were more severe within cell ultrastructures of Cutai compared to Sijiu. A sequential growth test also revealed that Sijiu was more tolerant to CIP stress compared to Cutai. Meanwhile, significantly higher (p < 0.05) root parameters and higher areas of the stele and xylem may be responsible for the increased uptake and transport of CIP in Sijiu. Ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) analysis revealed that CIP was metabolized to three major metabolites by the hydroxylation and breakdown of the piperazinyl substituent in the CIP molecule. The enhanced metabolic transformation of CIP in Sijiu indicated a more efficient capacity to detoxify, which in turn favored an increased accumulation of CIP in this cultivar. Thus, the present study demonstrated that the stronger tolerance and metabolism of Sijiu to CIP were responsible for its high CIP accumulation, suggesting an evolutionary mechanism for adaptation to environmental stress.

Authors+Show Affiliations

Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address: tchmo@jnu.edu.cn.Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29433110

Citation

Zhao, Hai-Ming, et al. "Intraspecific Variability of Ciprofloxacin Accumulation, Tolerance, and Metabolism in Chinese Flowering Cabbage (Brassica Parachinensis)." Journal of Hazardous Materials, vol. 349, 2018, pp. 252-261.
Zhao HM, Huang HB, Du H, et al. Intraspecific variability of ciprofloxacin accumulation, tolerance, and metabolism in Chinese flowering cabbage (Brassica parachinensis). J Hazard Mater. 2018;349:252-261.
Zhao, H. M., Huang, H. B., Du, H., Lin, J., Xiang, L., Li, Y. W., Cai, Q. Y., Li, H., Mo, C. H., Liu, J. S., Wong, M. H., & Zhou, D. M. (2018). Intraspecific variability of ciprofloxacin accumulation, tolerance, and metabolism in Chinese flowering cabbage (Brassica parachinensis). Journal of Hazardous Materials, 349, 252-261. https://doi.org/10.1016/j.jhazmat.2018.01.015
Zhao HM, et al. Intraspecific Variability of Ciprofloxacin Accumulation, Tolerance, and Metabolism in Chinese Flowering Cabbage (Brassica Parachinensis). J Hazard Mater. 2018 05 5;349:252-261. PubMed PMID: 29433110.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intraspecific variability of ciprofloxacin accumulation, tolerance, and metabolism in Chinese flowering cabbage (Brassica parachinensis). AU - Zhao,Hai-Ming, AU - Huang,He-Biao, AU - Du,Huan, AU - Lin,Jing, AU - Xiang,Lei, AU - Li,Yan-Wen, AU - Cai,Quan-Ying, AU - Li,Hui, AU - Mo,Ce-Hui, AU - Liu,Jie-Sheng, AU - Wong,Ming-Hung, AU - Zhou,Dong-Mei, Y1 - 2018/01/09/ PY - 2017/06/28/received PY - 2018/01/04/revised PY - 2018/01/06/accepted PY - 2018/2/13/pubmed PY - 2019/10/23/medline PY - 2018/2/13/entrez KW - Accumulation variation KW - Antibiotics KW - Chinese flowering cabbage (Brassica parachinensis) KW - Ciprofloxacin KW - Metabolism SP - 252 EP - 261 JF - Journal of hazardous materials JO - J. Hazard. Mater. VL - 349 N2 - To investigate the mechanism of genotype differences in ciprofloxacin (CIP) accumulation, this study was designed to compare the tolerance and metabolic responses to CIP exposure between low (Cutai) and high (Sijiu) CIP-accumulation cultivars of Brassica parachinensis. Decreases in biomass and chlorophyll content were significantly greater (p < 0.05) and toxicities were more severe within cell ultrastructures of Cutai compared to Sijiu. A sequential growth test also revealed that Sijiu was more tolerant to CIP stress compared to Cutai. Meanwhile, significantly higher (p < 0.05) root parameters and higher areas of the stele and xylem may be responsible for the increased uptake and transport of CIP in Sijiu. Ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) analysis revealed that CIP was metabolized to three major metabolites by the hydroxylation and breakdown of the piperazinyl substituent in the CIP molecule. The enhanced metabolic transformation of CIP in Sijiu indicated a more efficient capacity to detoxify, which in turn favored an increased accumulation of CIP in this cultivar. Thus, the present study demonstrated that the stronger tolerance and metabolism of Sijiu to CIP were responsible for its high CIP accumulation, suggesting an evolutionary mechanism for adaptation to environmental stress. SN - 1873-3336 UR - https://www.unboundmedicine.com/medline/citation/29433110/Intraspecific_variability_of_ciprofloxacin_accumulation_tolerance_and_metabolism_in_Chinese_flowering_cabbage__Brassica_parachinensis__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3894(18)30015-3 DB - PRIME DP - Unbound Medicine ER -