Tags

Type your tag names separated by a space and hit enter

Protective effect of dietary Alchemilla mollis on UVB-irradiated premature skin aging through regulation of transcription factor NFATc1 and Nrf2/ARE pathways.
Phytomedicine. 2018 Jan 15; 39:125-136.P

Abstract

BACKGROUND

Alchemilla mollis (lady's mantle) is a common ingredient in skin care products. However, the protective mechanism of A. mollis against skin problems has not been elucidated.

PURPOSE

This study was to investigate the effects of A. mollis ethanolic extract (AM) on UVB-irradiated normal human dermal fibroblasts (NHDF) and hairless mice.

METHODS

The in vitro anti-photoaging effect of AM was performed in NHDFs. The antioxidant activities were assessed through DPPH, ABTS, and reactive oxygen species (ROS) assays. Matrix metalloproteinase 1 (MMP-1), IL-6, procollagen type I, and transforming growth factor-β1 (TGF-β1) were measured by kits. The protein levels of p-c-Jun, p-c-Fos, Nrf2, NQO-1, HO-1, nuclear NFATc1 and cytosolic p-NFATc1 were evaluated by western blotting. In in vivo, H&E and Masson's trichrome staining were carried out. Skin texture was analyzed using the roughness parameters. The expression of MMP-1, procollagen type I, TGF-β1 and elastin were measured by western blot.

RESULTS

AM included gallic acid as an active constituent. AM exhibited a strong antioxidant effect by inhibiting DPPH and ABTS free radicals, as well as ROS production. It was also found to upregulate transforming growth factor β1, type I procollagen and elastin expression, and to downregulate matrix metalloproteinase-1 and interleukin-6 expression in AM-treated NHDFs under UVB irradiation. These effects were attributed to AP-1 and Nrf2/ARE signaling pathways. Significantly, it was demonstrated that AM regulated the UVB-induced NFATc1 dephosphorylation in nucleus. Based on dietary data, AM was effective for the prevention of wrinkle formation, skin thickening, water loss, and erythema in UVB-exposed mouse skin.

CONCLUSION

Our data indicate that A. mollis provides protection from UVB exposure in both hairless mice skin in vivo and NHDFs in vitro. AM might therefore be useful as a cosmetic material and functional food for the prevention of UVB-induced human skin photoaging.

Authors+Show Affiliations

SD Biotechnologies Co., Ltd., #301 Seoul Hightech Venture Center, 29, Gonghang-daero 61-gil, Ganseo-gu, Seoul 07563, Republic of Korea.College of Life Sciences, Kyung Hee University, 1732, Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Republic of Korea.College of Life Sciences, Kyung Hee University, 1732, Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Republic of Korea.College of Life Sciences, Kyung Hee University, 1732, Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Republic of Korea.College of Life Sciences, Kyung Hee University, 1732, Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Republic of Korea.College of Life Sciences, Kyung Hee University, 1732, Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Republic of Korea. Electronic address: drhoo@khu.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29433674

Citation

Hwang, Eunson, et al. "Protective Effect of Dietary Alchemilla Mollis On UVB-irradiated Premature Skin Aging Through Regulation of Transcription Factor NFATc1 and Nrf2/ARE Pathways." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 39, 2018, pp. 125-136.
Hwang E, Ngo HTT, Seo SA, et al. Protective effect of dietary Alchemilla mollis on UVB-irradiated premature skin aging through regulation of transcription factor NFATc1 and Nrf2/ARE pathways. Phytomedicine. 2018;39:125-136.
Hwang, E., Ngo, H. T. T., Seo, S. A., Park, B., Zhang, M., & Yi, T. H. (2018). Protective effect of dietary Alchemilla mollis on UVB-irradiated premature skin aging through regulation of transcription factor NFATc1 and Nrf2/ARE pathways. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 39, 125-136. https://doi.org/10.1016/j.phymed.2017.12.025
Hwang E, et al. Protective Effect of Dietary Alchemilla Mollis On UVB-irradiated Premature Skin Aging Through Regulation of Transcription Factor NFATc1 and Nrf2/ARE Pathways. Phytomedicine. 2018 Jan 15;39:125-136. PubMed PMID: 29433674.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of dietary Alchemilla mollis on UVB-irradiated premature skin aging through regulation of transcription factor NFATc1 and Nrf2/ARE pathways. AU - Hwang,Eunson, AU - Ngo,Hien T T, AU - Seo,Seul A, AU - Park,Bom, AU - Zhang,Mengyang, AU - Yi,Tae-Hoo, Y1 - 2017/12/26/ PY - 2017/08/09/received PY - 2017/11/08/revised PY - 2017/12/25/accepted PY - 2018/2/14/entrez PY - 2018/2/13/pubmed PY - 2018/8/8/medline KW - Alchemilla mollis KW - Anti-aging KW - NFATc1 KW - Nrf2/ARE KW - Skin KW - UVB SP - 125 EP - 136 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 39 N2 - BACKGROUND: Alchemilla mollis (lady's mantle) is a common ingredient in skin care products. However, the protective mechanism of A. mollis against skin problems has not been elucidated. PURPOSE: This study was to investigate the effects of A. mollis ethanolic extract (AM) on UVB-irradiated normal human dermal fibroblasts (NHDF) and hairless mice. METHODS: The in vitro anti-photoaging effect of AM was performed in NHDFs. The antioxidant activities were assessed through DPPH, ABTS, and reactive oxygen species (ROS) assays. Matrix metalloproteinase 1 (MMP-1), IL-6, procollagen type I, and transforming growth factor-β1 (TGF-β1) were measured by kits. The protein levels of p-c-Jun, p-c-Fos, Nrf2, NQO-1, HO-1, nuclear NFATc1 and cytosolic p-NFATc1 were evaluated by western blotting. In in vivo, H&E and Masson's trichrome staining were carried out. Skin texture was analyzed using the roughness parameters. The expression of MMP-1, procollagen type I, TGF-β1 and elastin were measured by western blot. RESULTS: AM included gallic acid as an active constituent. AM exhibited a strong antioxidant effect by inhibiting DPPH and ABTS free radicals, as well as ROS production. It was also found to upregulate transforming growth factor β1, type I procollagen and elastin expression, and to downregulate matrix metalloproteinase-1 and interleukin-6 expression in AM-treated NHDFs under UVB irradiation. These effects were attributed to AP-1 and Nrf2/ARE signaling pathways. Significantly, it was demonstrated that AM regulated the UVB-induced NFATc1 dephosphorylation in nucleus. Based on dietary data, AM was effective for the prevention of wrinkle formation, skin thickening, water loss, and erythema in UVB-exposed mouse skin. CONCLUSION: Our data indicate that A. mollis provides protection from UVB exposure in both hairless mice skin in vivo and NHDFs in vitro. AM might therefore be useful as a cosmetic material and functional food for the prevention of UVB-induced human skin photoaging. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/29433674/Protective_effect_of_dietary_Alchemilla_mollis_on_UVB_irradiated_premature_skin_aging_through_regulation_of_transcription_factor_NFATc1_and_Nrf2/ARE_pathways_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-7113(17)30199-X DB - PRIME DP - Unbound Medicine ER -