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Vaginal lactobacilli profile in pregnant women with normal & abnormal vaginal flora.
Indian J Med Res. 2017 10; 146(4):534-540.IJ

Abstract

Background & objectives

Lactobacilli species that are better adapted to vaginal environment of women may colonize better and offer protection against vaginal pathogenic bacteria. In this study, the distribution of common Lactobacillus species was investigated in pregnant women.

Methods

Sixty seven pregnant women were included in the study and vaginal samples were collected for Gram staining. Women were classified as normal vaginal flora, intermediate flora and bacterial vaginosis (BV) based on Nugent's score. Vaginal samples were also collected for the identification of Lactobacillus spp. by multiplex polymerase chain reaction (PCR) profiling of 16S rDNA amplification method.

Results

Lactobacillus crispatus (100%) was the most predominant Lactobacillus spp. present in pregnant women with normal flora, followed by L. iners (77%), L. jensenii (74%) and L. helveticus (60%). While, L. iners was commonly present across groups in women with normal, intermediate or BV flora, L. crispatus, L. jensenii and L. helveticus decreased significantly as the vaginal flora changed to intermediate and BV. In women with BV, except L. iners other species of lactobacilli was less frequently prevalent. Species such as L. rhamnosus, L. fermentum, L. paracasei and L. casei were not detected in any vaginal sample.

Interpretation & conclusions

L. crispatus, L. jensinii and L. helveticus were predominant species in women with normal flora. L. crispatus alone or in combination with L. jensinii and L. helveticus may be evaluated for probiotic properties for the prevention and treatment of BV.

Authors+Show Affiliations

Department of Microbiology & Immunology, ICMR-National Institute of Nutrition, Hyderabad, India.Department of Microbiology & Immunology, ICMR-National Institute of Nutrition, Hyderabad, India.Department of Microbiology & Immunology, ICMR-National Institute of Nutrition, Hyderabad, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29434069

Citation

Yeruva, Thirupathaiah, et al. "Vaginal Lactobacilli Profile in Pregnant Women With Normal & Abnormal Vaginal Flora." The Indian Journal of Medical Research, vol. 146, no. 4, 2017, pp. 534-540.
Yeruva T, Rajkumar H, Donugama V. Vaginal lactobacilli profile in pregnant women with normal & abnormal vaginal flora. Indian J Med Res. 2017;146(4):534-540.
Yeruva, T., Rajkumar, H., & Donugama, V. (2017). Vaginal lactobacilli profile in pregnant women with normal & abnormal vaginal flora. The Indian Journal of Medical Research, 146(4), 534-540. https://doi.org/10.4103/ijmr.IJMR_774_16
Yeruva T, Rajkumar H, Donugama V. Vaginal Lactobacilli Profile in Pregnant Women With Normal & Abnormal Vaginal Flora. Indian J Med Res. 2017;146(4):534-540. PubMed PMID: 29434069.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vaginal lactobacilli profile in pregnant women with normal & abnormal vaginal flora. AU - Yeruva,Thirupathaiah, AU - Rajkumar,Hemalatha, AU - Donugama,Vasundhara, PY - 2018/2/14/entrez PY - 2018/2/13/pubmed PY - 2018/9/15/medline KW - Bacterial vaginosis - Lactobacillus crispatus- multiplex polymerase chain reaction - Nugent score - pregnant women - vaginal Lactobacillus SP - 534 EP - 540 JF - The Indian journal of medical research JO - Indian J Med Res VL - 146 IS - 4 N2 - Background & objectives: Lactobacilli species that are better adapted to vaginal environment of women may colonize better and offer protection against vaginal pathogenic bacteria. In this study, the distribution of common Lactobacillus species was investigated in pregnant women. Methods: Sixty seven pregnant women were included in the study and vaginal samples were collected for Gram staining. Women were classified as normal vaginal flora, intermediate flora and bacterial vaginosis (BV) based on Nugent's score. Vaginal samples were also collected for the identification of Lactobacillus spp. by multiplex polymerase chain reaction (PCR) profiling of 16S rDNA amplification method. Results: Lactobacillus crispatus (100%) was the most predominant Lactobacillus spp. present in pregnant women with normal flora, followed by L. iners (77%), L. jensenii (74%) and L. helveticus (60%). While, L. iners was commonly present across groups in women with normal, intermediate or BV flora, L. crispatus, L. jensenii and L. helveticus decreased significantly as the vaginal flora changed to intermediate and BV. In women with BV, except L. iners other species of lactobacilli was less frequently prevalent. Species such as L. rhamnosus, L. fermentum, L. paracasei and L. casei were not detected in any vaginal sample. Interpretation & conclusions: L. crispatus, L. jensinii and L. helveticus were predominant species in women with normal flora. L. crispatus alone or in combination with L. jensinii and L. helveticus may be evaluated for probiotic properties for the prevention and treatment of BV. SN - 0971-5916 UR - https://www.unboundmedicine.com/medline/citation/29434069/Vaginal_lactobacilli_profile_in_pregnant_women_with_normal_&_abnormal_vaginal_flora_ DB - PRIME DP - Unbound Medicine ER -