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Functionalized Au@Ag-Au nanoparticles as an optical and SERS dual probe for lateral flow sensing.
Anal Bioanal Chem 2018; 410(9):2291-2303AB

Abstract

Lateral flow assay strips (LFASs) with Au nanoparticles (NPs) have been widely used as a probe for biomarkers in point-of-care testing; however, there still remain challenges in detection sensitivity and quantitative analysis. In this study, we developed a surface-enhanced Raman scattering (SERS)-based LFAS for quantitative analysis of a biomarker in the low concentration range. Moreover, apart from conventional Au NPs, three other types of citrate-capped Au-Ag bimetallic NPs: Au core with Ag shell NPs (Au@Ag NPs), rattle-like Au core in Ag-Au shell NPs (Au@Ag-Au NPs) and Ag-Au NPs were prepared and functionalized, and their solution-based SERS activities were comprehensively studied by experimental measurement and theoretical analysis. The results clearly indicated that the citrate-capped Au@Ag-Au NPs exhibited the highest SERS activity among the probes tested. Au@Ag-Au NPs were used as both optical and SERS probes in a SERS-based LFAS. In the presence of the analyte at high concentrations, a purple color appeared in the test zone. Highly sensitive and quantitative analysis was realized by measurement of SERS signals from the test lines. One of the most specific markers for cardiac injury, cardiac troponin I (cTnI), was chosen as the detection model. The detection limit of the SERS-based LFAS for cardiac troponin I was 0.09 ng/mL, lowered by nearly 50 times compared with visual results, and could be further lowered by optimization. These results demonstrated that the SERS-based LFAS using citrate-capped Au@Ag-Au NPs as probes can be a powerful tool for highly sensitive and quantitative detection of biomarkers. Graphical abstract A surface-enhanced Raman scattering (SERS)-based lateral flow assay strip using rattle-like Au core in Ag-Au shell (Au@Ag-Au) nanoparticles as probes was developed for quantitative analysis of a biomarker, with a detection limit nearly 50 times lower than that of visual assessment. C control line, T test line.

Authors+Show Affiliations

Department of Geriatrics, The Second Affiliated Hospital, Key Laboratory for Aging & Disease, Nanjing Medical University, Nanjing, Jiangsu, 210011, China.School of Science, Nantong University, Nantong, Jiangsu, 226007, China.School of Science, Nantong University, Nantong, Jiangsu, 226007, China.Department of Geriatrics, The Second Affiliated Hospital, Key Laboratory for Aging & Disease, Nanjing Medical University, Nanjing, Jiangsu, 210011, China.Department of Geriatrics, The Second Affiliated Hospital, Key Laboratory for Aging & Disease, Nanjing Medical University, Nanjing, Jiangsu, 210011, China.Department of Geriatrics, The Second Affiliated Hospital, Key Laboratory for Aging & Disease, Nanjing Medical University, Nanjing, Jiangsu, 210011, China.Department of Geriatrics, The Second Affiliated Hospital, Key Laboratory for Aging & Disease, Nanjing Medical University, Nanjing, Jiangsu, 210011, China.Department of Geriatrics, The Second Affiliated Hospital, Key Laboratory for Aging & Disease, Nanjing Medical University, Nanjing, Jiangsu, 210011, China.Department of Geriatrics, The Second Affiliated Hospital, Key Laboratory for Aging & Disease, Nanjing Medical University, Nanjing, Jiangsu, 210011, China. zhiruiguo@njmu.edu.cn.Department of Geriatrics, The Second Affiliated Hospital, Key Laboratory for Aging & Disease, Nanjing Medical University, Nanjing, Jiangsu, 210011, China. luxiang66@njmu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29445833

Citation

Bai, Tingting, et al. "Functionalized Au@Ag-Au Nanoparticles as an Optical and SERS Dual Probe for Lateral Flow Sensing." Analytical and Bioanalytical Chemistry, vol. 410, no. 9, 2018, pp. 2291-2303.
Bai T, Wang M, Cao M, et al. Functionalized Au@Ag-Au nanoparticles as an optical and SERS dual probe for lateral flow sensing. Anal Bioanal Chem. 2018;410(9):2291-2303.
Bai, T., Wang, M., Cao, M., Zhang, J., Zhang, K., Zhou, P., ... Lu, X. (2018). Functionalized Au@Ag-Au nanoparticles as an optical and SERS dual probe for lateral flow sensing. Analytical and Bioanalytical Chemistry, 410(9), pp. 2291-2303. doi:10.1007/s00216-018-0850-z.
Bai T, et al. Functionalized Au@Ag-Au Nanoparticles as an Optical and SERS Dual Probe for Lateral Flow Sensing. Anal Bioanal Chem. 2018;410(9):2291-2303. PubMed PMID: 29445833.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functionalized Au@Ag-Au nanoparticles as an optical and SERS dual probe for lateral flow sensing. AU - Bai,Tingting, AU - Wang,Meng, AU - Cao,Min, AU - Zhang,Juan, AU - Zhang,Kangzhen, AU - Zhou,Ping, AU - Liu,Zhengxia, AU - Liu,Ying, AU - Guo,Zhirui, AU - Lu,Xiang, Y1 - 2018/02/14/ PY - 2017/10/26/received PY - 2018/01/03/accepted PY - 2017/12/05/revised PY - 2018/2/16/pubmed PY - 2018/5/2/medline PY - 2018/2/16/entrez KW - Au nanoparticles KW - Au-Ag bimetallic nanoparticles KW - Lateral flow assay strip KW - Surface-enhanced Raman scattering SP - 2291 EP - 2303 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 410 IS - 9 N2 - Lateral flow assay strips (LFASs) with Au nanoparticles (NPs) have been widely used as a probe for biomarkers in point-of-care testing; however, there still remain challenges in detection sensitivity and quantitative analysis. In this study, we developed a surface-enhanced Raman scattering (SERS)-based LFAS for quantitative analysis of a biomarker in the low concentration range. Moreover, apart from conventional Au NPs, three other types of citrate-capped Au-Ag bimetallic NPs: Au core with Ag shell NPs (Au@Ag NPs), rattle-like Au core in Ag-Au shell NPs (Au@Ag-Au NPs) and Ag-Au NPs were prepared and functionalized, and their solution-based SERS activities were comprehensively studied by experimental measurement and theoretical analysis. The results clearly indicated that the citrate-capped Au@Ag-Au NPs exhibited the highest SERS activity among the probes tested. Au@Ag-Au NPs were used as both optical and SERS probes in a SERS-based LFAS. In the presence of the analyte at high concentrations, a purple color appeared in the test zone. Highly sensitive and quantitative analysis was realized by measurement of SERS signals from the test lines. One of the most specific markers for cardiac injury, cardiac troponin I (cTnI), was chosen as the detection model. The detection limit of the SERS-based LFAS for cardiac troponin I was 0.09 ng/mL, lowered by nearly 50 times compared with visual results, and could be further lowered by optimization. These results demonstrated that the SERS-based LFAS using citrate-capped Au@Ag-Au NPs as probes can be a powerful tool for highly sensitive and quantitative detection of biomarkers. Graphical abstract A surface-enhanced Raman scattering (SERS)-based lateral flow assay strip using rattle-like Au core in Ag-Au shell (Au@Ag-Au) nanoparticles as probes was developed for quantitative analysis of a biomarker, with a detection limit nearly 50 times lower than that of visual assessment. C control line, T test line. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/29445833/Functionalized_Au@Ag_Au_nanoparticles_as_an_optical_and_SERS_dual_probe_for_lateral_flow_sensing_ L2 - https://dx.doi.org/10.1007/s00216-018-0850-z DB - PRIME DP - Unbound Medicine ER -