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Chromosomal distribution of pTa-535, pTa-86, pTa-713, 35S rDNA repetitive sequences in interspecific hexaploid hybrids of common wheat (Triticum aestivum L.) and spelt (Triticum spelta L.).
PLoS One. 2018; 13(2):e0192862.Plos

Abstract

Fluorescent in situ hybridization (FISH) relies on fluorescent-labeled probes to detect specific DNA sequences in the genome, and it is widely used in cytogenetic analyses. The aim of this study was to determine the karyotype of T. aestivum and T. spelta hybrids and their parental components (three common wheat cultivars and five spelt breeding lines), to identify chromosomal aberrations in the evaluated wheat lines, and to analyze the distribution of polymorphisms of repetitive sequences in the examined hybrids. The FISH procedure was carried out with four DNA clones, pTa-86, pTa-535, pTa-713 and 35S rDNA used as probes. The observed polymorphisms between the investigated lines of common wheat, spelt and their hybrids was relatively low. However, differences were observed in the distribution of repetitive sequences on chromosomes 4A, 6A, 1B and 6B in selected hybrid genomes. The polymorphisms observed in common wheat and spelt hybrids carry valuable information for wheat breeders. The results of our study are also a valuable source of knowledge about genome organization and diversification in common wheat, spelt and their hybrids. The relevant information is essential for common wheat breeders, and it can contribute to breeding programs aimed at biodiversity preservation.

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Authors+Show Affiliations

Goriewa-Duba K
Department of Plant Breeding and Seed Production, University of Warmia and Mazury in Olsztyn, Olsztyn, Warmian-Masurian Voivodeship, Poland.
Duba A
Department of Entomology, Phytopathology and Molecular Diagnostics, University of Warmia and Mazury in Olsztyn, Olsztyn, Warmian-Masurian Voivodeship, Poland.
Kwiatek M
Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Wielkopolskie Voivodeship, Poland. Department of Genetics and Plant Breeding, Poznań University of Life Sciences, Poznań, Wielkopolskie Voivodeship, Poland.
Wiśniewska H
Department of Genetics and Plant Breeding, Poznań University of Life Sciences, Poznań, Wielkopolskie Voivodeship, Poland.
Wachowska U
Department of Entomology, Phytopathology and Molecular Diagnostics, University of Warmia and Mazury in Olsztyn, Olsztyn, Warmian-Masurian Voivodeship, Poland.
Wiwart M
Department of Plant Breeding and Seed Production, University of Warmia and Mazury in Olsztyn, Olsztyn, Warmian-Masurian Voivodeship, Poland.

MeSH

Chromosomes, PlantHybridization, GeneticIn Situ Hybridization, FluorescencePlant BreedingPolyploidyRNA, RibosomalRepetitive Sequences, Nucleic AcidTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29447228

Citation

Goriewa-Duba, Klaudia, et al. "Chromosomal Distribution of pTa-535, pTa-86, pTa-713, 35S rDNA Repetitive Sequences in Interspecific Hexaploid Hybrids of Common Wheat (Triticum Aestivum L.) and Spelt (Triticum Spelta L.)." PloS One, vol. 13, no. 2, 2018, pp. e0192862.
Goriewa-Duba K, Duba A, Kwiatek M, et al. Chromosomal distribution of pTa-535, pTa-86, pTa-713, 35S rDNA repetitive sequences in interspecific hexaploid hybrids of common wheat (Triticum aestivum L.) and spelt (Triticum spelta L.). PLoS One. 2018;13(2):e0192862.
Goriewa-Duba, K., Duba, A., Kwiatek, M., Wiśniewska, H., Wachowska, U., & Wiwart, M. (2018). Chromosomal distribution of pTa-535, pTa-86, pTa-713, 35S rDNA repetitive sequences in interspecific hexaploid hybrids of common wheat (Triticum aestivum L.) and spelt (Triticum spelta L.). PloS One, 13(2), e0192862. https://doi.org/10.1371/journal.pone.0192862
Goriewa-Duba K, et al. Chromosomal Distribution of pTa-535, pTa-86, pTa-713, 35S rDNA Repetitive Sequences in Interspecific Hexaploid Hybrids of Common Wheat (Triticum Aestivum L.) and Spelt (Triticum Spelta L.). PLoS One. 2018;13(2):e0192862. PubMed PMID: 29447228.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chromosomal distribution of pTa-535, pTa-86, pTa-713, 35S rDNA repetitive sequences in interspecific hexaploid hybrids of common wheat (Triticum aestivum L.) and spelt (Triticum spelta L.). AU - Goriewa-Duba,Klaudia, AU - Duba,Adrian, AU - Kwiatek,Michał, AU - Wiśniewska,Halina, AU - Wachowska,Urszula, AU - Wiwart,Marian, Y1 - 2018/02/15/ PY - 2017/09/26/received PY - 2018/01/31/accepted PY - 2018/2/16/entrez PY - 2018/2/16/pubmed PY - 2018/4/24/medline SP - e0192862 EP - e0192862 JF - PloS one JO - PLoS One VL - 13 IS - 2 N2 - Fluorescent in situ hybridization (FISH) relies on fluorescent-labeled probes to detect specific DNA sequences in the genome, and it is widely used in cytogenetic analyses. The aim of this study was to determine the karyotype of T. aestivum and T. spelta hybrids and their parental components (three common wheat cultivars and five spelt breeding lines), to identify chromosomal aberrations in the evaluated wheat lines, and to analyze the distribution of polymorphisms of repetitive sequences in the examined hybrids. The FISH procedure was carried out with four DNA clones, pTa-86, pTa-535, pTa-713 and 35S rDNA used as probes. The observed polymorphisms between the investigated lines of common wheat, spelt and their hybrids was relatively low. However, differences were observed in the distribution of repetitive sequences on chromosomes 4A, 6A, 1B and 6B in selected hybrid genomes. The polymorphisms observed in common wheat and spelt hybrids carry valuable information for wheat breeders. The results of our study are also a valuable source of knowledge about genome organization and diversification in common wheat, spelt and their hybrids. The relevant information is essential for common wheat breeders, and it can contribute to breeding programs aimed at biodiversity preservation. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/29447228/Chromosomal_distribution_of_pTa_535_pTa_86_pTa_713_35S_rDNA_repetitive_sequences_in_interspecific_hexaploid_hybrids_of_common_wheat__Triticum_aestivum_L___and_spelt__Triticum_spelta_L___ DB - PRIME DP - Unbound Medicine ER -